蛋白質純化與抗體純化產品

為了分析單個蛋白質和蛋白質複合物(protein complexes)，識別蛋白質、蛋白質和核酸之間的相互作用，蛋白質純化(protein purification)是這些研究的cornerstone。由於天然蛋白質(natural proteins)的純化是一項具有挑戰性的任務，科學家們開發了一種實驗系統，該系統使用標籤融合蛋白(tag fusion proteins)來捕獲(capture)、純化(purify)和檢測目標蛋白。純化蛋白質最有效的方法是親和層析(affinity chromatography)，它通過目標蛋白質與匹配的固定化配體的特異性結合來達到純化的目的。最顯著的特點是，將一個特徵良好的標籤與其相應的固相偶聯配體結合，可以一步實現對標記蛋白的親和純化。帶有融合標籤的抗體也廣泛用於下游檢測和實驗，無需為每種特定的重組蛋白開發相應的探針。

抗體純化(Antibody purification)主要涉及從血清（多克隆抗體）、腹水(ascites) 和雜交瘤細胞系培養上清液(hybridoma cell line culture supernatants)（monoclonal antibodies, 單克隆抗體）中選擇性富集或特異性分離抗體。蛋白 A(Protein A)、蛋白 G(Protein G) 和蛋白 L (Protein L)是三種細菌蛋白(bacterial proteins)，它們以與抗體的有效結合而聞名。這些蛋白質通過基因工程編輯重組和表達，通常用於親和純化來自各種不同物種的關鍵抗體類型。針對蛋白純化(protein purification)和抗體純化(antibody purification)，提供填料(filler)、預裝離心柱(prepacked spin column )和試劑盒(kit)三種產品類型，以滿足不同的研究需求。

PurKine™ 抗體純化試劑盒（蛋白 A/G）PurKine™ Antibody Purification Kit (Protein A/G) | 貨號KTP2070

PurKine™ Antibody Purification Kit (Protein A/G)

PurKine™ Protein A/G Resin 4FF 已被固定用於各種規模的重力柱程序中的 IgG 純化應用。該樹脂由 90μm 的交聯 4% 瓊脂糖珠組成，並與重組蛋白 A/G 偶聯。測試證實其性能等於或超過其他供應商的流行重組蛋白 A/G 樹脂，並且在使用後性能沒有下降至少五次重複使用。此外，它的高流動性使其非常適合按比例放大。

產品描述：

PurKine™ 蛋白 A/G 純化系統(PurKine™ Protein A/G Purification system)可有效地從血清、腹水、細胞培養上清液和其他抗體樣品中親和純化 IgG。重組蛋白 A/G (Recombinant Protein A/G)採用專有方法進行化學修飾，以最大限度地減少蛋白質的非特異性結合。此外，Protein A/G 與免疫球蛋白的結合不像 Protein A 那樣依賴於 pH。Protein A/G 與所有人類 IgG 亞類結合併與所有小鼠 IgG 亞類很好地結合，但不與小鼠 IgA、IgM 或血清白蛋白結合。該產品組合允許優化工藝以獲得最大的蛋白質產量、穩定性和溶解度。測試還證實，在至少五次重複使用後性能沒有下降。 PurKine™ 蛋白 A/G 純化樹脂也可作為預裝離心柱(prepacked spin column)和試劑盒形式(kit formats)提供。

Product name	PurKine™ Antibody Purification Kit (Protein A/G)
Applications notes	PurKine™ Protein A/G Resin 4FF has been immobilized for use in IgG purification applications in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of cross-linked 4% agarose, to which Recombinant protein A/G has been coupled.Tests confirm that performance equals or exceeds popular Recombinant protein A/G resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.In addition, its high flow properties make it excellent for scaling-up.
Alternative	Protein A/G; Protein A/G resin
Kit components	• PurKine™ Protein A/G Packed Column • Binding/Wash buffer(10X) • Elution buffer(10x)
Features & Benefits	• High capacity—More than 7mg human lgG per mL of resin. • High performance—Recombinant Protein A/G Beads combines the IgG binding domains of both Protein A and Protein G. Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more general and convenient tool for investigating and purifying immunoglobulins. • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits. • Robust—highly crosslinked beads tolerate linear flow rates up to 300cm/hour. • Easy to use— Pre-formulated buffers available for kit formats.
Storage instructions	Stable for one year at 2-8°C from date of shipment. Do not freeze.
Background	PurKine™ Protein A/G Purification system is effective for affinity purification of IgG from serum, ascites fluid, cell culture supernantant and other antibody samples. Recombinant Protein A/G is chemically modified with a proprietary method to minimize nonspecific binding of proteins. Also, Protein A/G binding to immunoglobulins is not as pH-dependant as Protein A. Protein A/G binds to all human IgG subclasses and binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM or serum albumin. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Protein A/G Purification Resin is also available as prepacked spin column and kit formats.
Alternative	Protein A/G; Protein A/G resin

• Protein Quantification

  Protein quantification series products include protein quantification kits, WB developing solution, ECL, color pre-stained protein marker, etc.

• Protein Purification

  For protein purification and antibody purification, there are three product types: filler, prepacked spin column and kit to meet different research needs.

• Protein Labeling

  Directly label enzymes, biotin and fluorescent dyes on antibodies, proteins, peptides or other biomolecules to meet the needs of scientific research, drug development and diagnostic kit development.

• Protein Detection(Antibody & Kit)

  The antibodies provided by Abbkine for protein detection include internal reference antibody, tag antibody, primary antibody, secondary antibody and sealed serum.

• Protein Extraction

  Protein extraction is the starting point for immunology/cytology experiments.

PurKine™ His-Tag Ni-NTA Resin	BMR2001
PurKine™ His-Tag Protein Purification Kit (Ni-NTA)	KTP2001
PurKine™ GST-Tag Glutathione Resin	BMR2010
PurKine™ GST-Tag Protein Purification Kit (Glutathione)	KTP2010
PurKine™ MBP-Tag Dextrin Resin	BMR2020
PurKine™ MBP-Tag Protein Purification Kit (Dextrin)	KTP2020
PurKine™ Biotin-Tag Streptavidin Resin 6FF	BMR2030
PurKine™ Biotin-Tag Protein Purification Kit (Streptavidin)	KTP2030
PurKine™ Strep II-Tag Strep-Tactin Resin 4FF	BMR2040
PurKine™ Protein A/G Resin 4FF	BMR2070
PurKine™ Antibody Purification Kit (Protein A/G)	KTP2070
PurKine™ Endotoxin Removal Resin	BMR2140
PurKine™ Endotoxin Removal Kit (Polymyxin B)	KTP2140
Ulp1 (SUMO Protease /Ulp1 peptidase)	PRP3001

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

產品貨號	品項	反應	測量儀器
貨號KTA1001	活細胞和死細胞雙染色試劑盒	100個反應	活細胞綠色螢光染劑Ex/Em = 488/530 nm; 死細胞紅色螢光染料(Ex/Em = 535/617)
貨號 KTA2010	TUNEL細胞凋亡檢測試劑盒	50個反應	螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道（Ex / Em = 490nm / 520 nm）
貨號 KTC011001	CCK-8細胞增殖和細胞毒性試劑盒	500個反應	吸收光450nm
貨號KTC4003	TraKine™粒線體染色	50個反應/250個反應	Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	50個反應/250個反應	Ex/Em = 500/520 nm
貨號KTC4001	Abbkine 細胞膜染色	100個反應/500個反應	Ex/Em = 484/501 nm
貨號BMD00063	DAPI Staining Solution	10MG/100MG	Ex/Em: 358/461 nm (with DNA)
貨號KTC4210	活細胞Lysosome染色	50個反應/250個反應	deep red fluorescent dye , Ex/Em:650/665nm
貨號KTA3030	細胞衰老檢測試劑盒（β-半乳糖苷酶）	100個反應	光學顯微鏡
貨號KTA1020	CCK-8細胞增殖和細胞毒性試劑盒	1000個反應/10,000個反應	吸收光450nm
貨號KTA2030	細胞增殖EdU Image試劑盒 (BrdU替代法)	100個反應/500個反應	AbFluor 488 azide (Ex/Em = 501/525 nm)
貨號KTB1400	一氧化Dan（NO）檢測試劑盒	96個反應/ 192個反應	colorimetric (540 nm) 檢測範圍：1-100 µM
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/
貨號KTD3001	BCA蛋白定量試劑盒	2000個反應/ 5000個反應	OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 µg/ml.
貨號KTB1110	乳酸脫氫酶（LDH）測定試劑盒	96個反應/ 480個反應	λmax= 450 nm 1- 20 U/mL of LDH
貨號PRP100153	IGF-I重組蛋白	200UG/1MG	human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) > 95 % as determined by SDS-PAGE
貨號 KTA2020	細胞週期染色檢測	100個反應/500個反應	Ex/Em=535/615 nm.
貨號BMC21400	PurKine™內毒素去除預裝離心柱	1ml×5 3mlx5	More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
貨號KTA1200	CheKine™蛋白羰基比色測定試劑盒	48個反應/ 96個反應	吸收光370 nm
貨號KTB1080	超氧陰離子清除測定試劑盒	48個反應/ 96個反應	Superoxide anion scavenging rate D% =（∆∆ODControl – ∆∆ODSample）÷ ∆∆ODControl ×100%
貨號KTB1090	清除氫氧自由基能力測定試劑	48個反應/ 96個反應	吸收光 536nm Hydroxyl radical scavenging rate D% =（ASample – AControl）÷（ABlank – AControl）×100%
貨號KTB2220	TC總膽固醇檢測試劑盒	48個反應/ 96個反應	500nm Standard: 5 μmol/mL
貨號KTB1510	CheKine™尿酸（UA）比色分析試劑盒	48個反應/ 96個反應	吸收光505 nm Standard: 5 μmol/mL.
貨號KTB1300	Glucose葡萄糖測定試劑盒	96個反應/ 192個反應	吸光值630nm 4- 300 mg/dL)
貨號KTB1100	Lactate  乳酸檢測試劑盒	96個反應/ 480個反應	colorimetric (450 nm) 0.03 mM-2 mM
貨號KTB1700	組織和血液鹼性磷酸酶（ Alkaline Phosphatase, AKP / ALP）比色測定	96個反應/ 96 X5盤	吸收光510 nm
貨號KTB1500	總抗氧化劑能力TCA分析	96個反應/ 480個反應	593 nm 偵測範圍: 0.15-3 mM
貨號KTA0002	Annexin V-AbFluor™488細胞凋亡檢測試劑盒	20個反應/50個反應/100個反應	Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
貨號KET7013	EliKine™ Rat IL-6 ELISA Kit試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL
貨號KET0003	雌二醇（Estradiol , E2）試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍40 pg/ml-1500 pg/ml
貨號KET6032	TNF–α Alpha型腫瘤壞死因子 ELISA試劑盒	96個反應/ 96 X5盤	450 nm 7.8 pg/ml-500 pg/ml
貨號KET6033	VEGF血管內皮生長因子ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 31.25 pg/mL-2000 pg/mL
貨號KET7005	白介素-1β（Interleukin-1β, IL-1β）ELISA試劑盒	96個反應/ 96 X5盤	Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. 偵測範圍: 15.6 pg/ml-1000 pg/ml
貨號KTE70003	小鼠黃嘌呤脫氫酶/氧化酶（XDH）ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。
貨號 A02051HRP	His-tag標籤多株抗體-HRP	50ul/200ul	WB (1:5000).
貨號A02170MGB	Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠	100ul/400ul/2ml	GKPIPNPLLGLDST epitope
貨號KTA4001	JC-1線粒體膜電位測定試劑盒	100個反應/ 500個反應	（聚結的JC-1，Ex / Em = 585/590 nm）發出綠色螢光（JC-1單體，Ex / Em = 510/527 nm） JC-1 Stain CCCP (10 mM)  Assay Buffer (5×)
貨號KTB1600	穀胱甘肽(Glutathione)檢測	96個反應	412 nm 2-200 µg/mL
貨號BMD00063	DAPI Staining Solution	1MG/10MG/100MG	λEx/λEm: 358/461 nm (with DNA)
貨號KTP3001	細胞核/細胞質分離試劑盒	50個反應/ 200個反應	Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)
貨號BMD00064-50UG	Calcein AM鈣黃綠素活細胞螢光染劑	50ug/1mg/10mg	λEX/λEm: 494/517 nm (pH 8) (calcein)
貨號 KTB1660	硫氧還蛋白過氧化物酶（TPX）檢測	96個反應	240nm
貨號KTB1020	NAD/NADH 分析試劑盒	96個反應/ 480個反應	OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/mL
貨號KTB1040	Catalase過氧化氫酶活性檢測試劑盒	96個反應/ 480個反應	OD 540 nm 2-75 µM
貨號KTB1060	過氧化物酶（Peroxidases）分析試劑盒	96個反應	OD 664 nm 線性範圍為15.6-1000 µU/mL
貨號KTB1050	脂質過氧化檢測試劑盒(MDA)	96個反應	OD 532 nm 1-100 µM/L
貨號KTB1030	超氧化物歧化酶（SOD）活性測定試劑盒	96個反應	200-3.13 U/ml
貨號KTA1030	LDH細胞毒性測定試劑盒	96個反應/ 480個反應	OD 565 nm 10,00-100,0000 cells per well