PurKine™ His-Tag 蛋白純化試劑盒 (Ni-NTA) | PurKine™ His-Tag Protein Purification Kit (Ni-NTA)貨號KTP2001

代理廠牌：
原廠連結：
相關下載：	PurKine™ His-Tag 蛋白純化試劑盒操作手冊

特點

產品描述：

PurKine™ His-Tag 純化系統(PurKine™ His-Tag Purification system)基於創新的高容量 IMAC 基質(IMAC matrix)，可方便地從總裂解物(total lysates)中一步純化 His-tag 蛋白。專有的離子螯合化學(ion-chelate chemistry)確保與常用的還原劑（reducing agents, 如 DTT）、螯合金屬蛋白酶抑製劑（chelating metalloprotease inhibitors, 如 EDTA）以及各種緩衝物質(buffer substances)和鹽條件(salt conditions)具有非凡的相容性。該產品組合提供多種螯合基團（IDA、NTA 或 Super NTA）、交聯瓊脂糖（交聯 4% 或 6% 瓊脂糖）、離子（Nick、Colbat、銅或非帶電離子）和兼容成分，以實現優化最大蛋白質產量、穩定性和溶解度的過程。測試還證實，在至少五次重複使用後性能沒有下降。 PurKine™ His-Tag 純化樹脂也可作為預裝離心柱(prepacked spin column)和試劑盒(kit)形式提供。

PurKine™ His-Tag 蛋白純化試劑盒(PurKine™ His-Tag Protein Purification Kit, KTP2001)可在各種規模的重力柱(gravity column)程序中有效純化高水平過表達的 His-tag 融合蛋白。試劑盒中的樹脂(Resin) 由 90 μm 高度交聯的 4% 瓊脂糖珠(highly cross-linked 4% agarose)組成，與硝酸三乙酸 (Nitilotriacetic acid , NTA) 偶聯。然後螯合基團帶有鎳離子 (nickel ions , Ni2+)。測試證實其性能等於或超過其他供應商的流行 Ni-NTA 樹脂(Ni-NTA resins)，並且在至少五次重複使用後性能沒有下降。

Product name	PurKine™ His-Tag Protein Purification Kit (Ni-NTA)
Applications notes	PurKine™ His-Tag Protein Purification Kit effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin in kit consists of 90μm beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.
Alternative	Ni-NTA; His resin; Ni-NTA resin; His tag resin
Kit components	• PurKine™ His-Tag Purification Nickel Column • Phosphate-Buffered Saline (PBS,10X) • Imidazole (2M)
Features & Benefits	• High capacity—More than 50 mg of 6xHis-tagged protein per milliliter of resin. •Versatile—For purification under native or denaturing conditions. • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits. • Easy to use— Pre-formulated buffers available for kit formats.
Storage instructions	Stable for one year at 2-8°C from date of shipment. Do not freeze.
Shipping	Blue ice
kground	PurKine™ His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ His-Tag Purification resin is also available as prepacked spin column and kit formats.
Alternative	Ni-NTA; His resin; Ni-NTA resin; His tag resin

活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒

貨號	產品名稱	胞器染色位置	螢光波段
貨號KTC4003	TraKine™粒線體染色	粒線體(mitochondria)	Ex / Em = 490/523 nm	增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	微管蛋白(Tubulin)	Ex/Em = 500/520 nm	活細胞或固定之細胞
貨號KTC4001	Abbkine 細胞膜染色	細胞膜 (plasma membrane)	Ex/Em = 484/501 nm	活細胞、固定懸浮、貼附細胞
貨號BMD00063	DAPI Staining Solution	細胞核 dsDNA	Ex/Em: 358/461 nm (with DNA)	染色活細胞和固定細胞
貨號KTC4210	活細胞Lysosome染色	Lysosome	deep red fluorescent dye , Ex/Em:650/665nm	染色活細胞和固定細胞

產品貨號	品項	反應	測量儀器
貨號KTA1001	活細胞和死細胞雙染色試劑盒	100個反應	活細胞綠色螢光染劑Ex/Em = 488/530 nm; 死細胞紅色螢光染料(Ex/Em = 535/617)
貨號 KTA2010	TUNEL細胞凋亡檢測試劑盒	50個反應	螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道（Ex / Em = 490nm / 520 nm）
貨號 KTC011001	CCK-8細胞增殖和細胞毒性試劑盒	500個反應	吸收光450nm
貨號KTC4003	TraKine™粒線體染色	50個反應/250個反應	Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	50個反應/250個反應	Ex/Em = 500/520 nm
貨號KTC4001	Abbkine 細胞膜染色	100個反應/500個反應	Ex/Em = 484/501 nm
貨號BMD00063	DAPI Staining Solution	10MG/100MG	Ex/Em: 358/461 nm (with DNA)
貨號KTC4210	活細胞Lysosome染色	50個反應/250個反應	deep red fluorescent dye , Ex/Em:650/665nm
貨號KTA3030	細胞衰老檢測試劑盒（β-半乳糖苷酶）	100個反應	光學顯微鏡
貨號KTA1020	CCK-8細胞增殖和細胞毒性試劑盒	1000個反應/10,000個反應	吸收光450nm
貨號KTA2030	細胞增殖EdU Image試劑盒 (BrdU替代法)	100個反應/500個反應	AbFluor 488 azide (Ex/Em = 501/525 nm)
貨號KTB1400	一氧化Dan（NO）檢測試劑盒	96個反應/ 192個反應	colorimetric (540 nm) 檢測範圍：1-100 µM
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/
貨號KTD3001	BCA蛋白定量試劑盒	2000個反應/ 5000個反應	OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 µg/ml.
貨號KTB1110	乳酸脫氫酶（LDH）測定試劑盒	96個反應/ 480個反應	λmax= 450 nm 1- 20 U/mL of LDH
貨號PRP100153	IGF-I重組蛋白	200UG/1MG	human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) > 95 % as determined by SDS-PAGE
貨號 KTA2020	細胞週期染色檢測	100個反應/500個反應	Ex/Em=535/615 nm.
貨號BMC21400	PurKine™內毒素去除預裝離心柱	1ml×5 3mlx5	More than 2,000,000 EU/mL to eliminate >99% of endotoxins.
貨號KTA1200	CheKine™蛋白羰基比色測定試劑盒	48個反應/ 96個反應	吸收光370 nm
貨號KTB1080	超氧陰離子清除測定試劑盒	48個反應/ 96個反應	Superoxide anion scavenging rate D% =（∆∆ODControl – ∆∆ODSample）÷ ∆∆ODControl ×100%
貨號KTB1090	清除氫氧自由基能力測定試劑	48個反應/ 96個反應	吸收光 536nm Hydroxyl radical scavenging rate D% =（ASample – AControl）÷（ABlank – AControl）×100%
貨號KTB2220	TC總膽固醇檢測試劑盒	48個反應/ 96個反應	500nm Standard: 5 μmol/mL
貨號KTB1510	CheKine™尿酸（UA）比色分析試劑盒	48個反應/ 96個反應	吸收光505 nm Standard: 5 μmol/mL.
貨號KTB1300	Glucose葡萄糖測定試劑盒	96個反應/ 192個反應	吸光值630nm 4- 300 mg/dL)
貨號KTB1100	Lactate 乳酸檢測試劑盒	96個反應/ 480個反應	colorimetric (450 nm) 0.03 mM-2 mM
貨號KTB1700	組織和血液鹼性磷酸酶（ Alkaline Phosphatase, AKP / ALP）比色測定	96個反應/ 96 X5盤	吸收光510 nm
貨號KTB1500	總抗氧化劑能力TCA分析	96個反應/ 480個反應	593 nm 偵測範圍: 0.15-3 mM
貨號KTA0002	Annexin V-AbFluor™488細胞凋亡檢測試劑盒	20個反應/50個反應/100個反應	Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
貨號KET7013	EliKine™ Rat IL-6 ELISA Kit試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL
貨號KET0003	雌二醇（Estradiol , E2）試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍40 pg/ml-1500 pg/ml
貨號KET6032	TNF–α Alpha型腫瘤壞死因子 ELISA試劑盒	96個反應/ 96 X5盤	450 nm 7.8 pg/ml-500 pg/ml
貨號KET6033	VEGF血管內皮生長因子ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 31.25 pg/mL-2000 pg/mL
貨號KET7005	白介素-1β（Interleukin-1β, IL-1β）ELISA試劑盒	96個反應/ 96 X5盤	Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. 偵測範圍: 15.6 pg/ml-1000 pg/ml
貨號KTE70003	小鼠黃嘌呤脫氫酶/氧化酶（XDH）ELISA試劑盒	96個反應/ 96 X5盤	450 nm 偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。
貨號 A02051HRP	His-tag標籤多株抗體-HRP	50ul/200ul	WB (1:5000).
貨號A02170MGB	Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠	100ul/400ul/2ml	GKPIPNPLLGLDST epitope
貨號KTA4001	JC-1線粒體膜電位測定試劑盒	100個反應/ 500個反應	（聚結的JC-1，Ex / Em = 585/590 nm）發出綠色螢光（JC-1單體，Ex / Em = 510/527 nm） JC-1 Stain CCCP (10 mM) Assay Buffer (5×)
貨號KTB1600	穀胱甘肽(Glutathione)檢測	96個反應	412 nm 2-200 µg/mL
貨號BMD00063	DAPI Staining Solution	1MG/10MG/100MG	λEx/λEm: 358/461 nm (with DNA)
貨號KTP3001	細胞核/細胞質分離試劑盒	50個反應/ 200個反應	Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)
貨號BMD00064-50UG	Calcein AM鈣黃綠素活細胞螢光染劑	50ug/1mg/10mg	λEX/λEm: 494/517 nm (pH 8) (calcein)
貨號 KTB1660	硫氧還蛋白過氧化物酶（TPX）檢測	96個反應	240nm
貨號KTB1020	NAD/NADH 分析試劑盒	96個反應/ 480個反應	OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。
貨號KTB1070	CheKine™黃嘌呤氧化酶檢測	96個反應/ 480個反應	OD 450 nm 線性範圍15.6 -500 mU/mL
貨號KTB1040	Catalase過氧化氫酶活性檢測試劑盒	96個反應/ 480個反應	OD 540 nm 2-75 µM
貨號KTB1060	過氧化物酶（Peroxidases）分析試劑盒	96個反應	OD 664 nm 線性範圍為15.6-1000 µU/mL
貨號KTB1050	脂質過氧化檢測試劑盒(MDA)	96個反應	OD 532 nm 1-100 µM/L
貨號KTB1030	超氧化物歧化酶（SOD）活性測定試劑盒	96個反應	200-3.13 U/ml
貨號KTA1030	LDH細胞毒性測定試劑盒	96個反應/ 480個反應	OD 565 nm 10,00-100,0000 cells per well

↑回到頁首↑