GAPDH Polyclonal Antibody | Abbkine貨號ABP52783
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GAPDH Polyclonal Antibody | Abbkine貨號ABP52783
Rabbit anti-GAPDH Polyclonal Antibody
辨視物種Human, Mouse, Rat。可應用於ELISA, IHC-P, WB實驗。
GAPDH 編碼 3-磷酸甘油醛脫氫酶蛋白家族(glyceraldehyde-3-phosphate dehydrogenase protein family)的成員。甘油醛-3-磷酸脫氫酶(Glyceraldehyde-3-phosphate dehydrogenase)已被確定為一種moonlighting protein，因為它能夠執行mechanistically distinct functions。 GAPDH 的產物催化碳水化合物代謝(carbohydrate metabolism)中重要的能量產生步驟，即在無機磷酸鹽(inorganic phosphate)和煙酰胺腺嘌呤二核苷酸 (nicotinamide adenine dinucleotide , NAD) 存在下 3-磷酸甘油醛(Glyceraldehyde-3-phosphate dehydrogenase)的可逆氧化磷酸化。已另外鑑定甘油醛-3-磷酸脫氫酶(Glyceraldehyde-3-phosphate dehydrogenase)在細胞核中具有尿嘧啶DNA糖基化酶(uracil DNA glycosylase activity)活性。此外，甘油醛-3-磷酸脫氫酶(Glyceraldehyde-3-phosphate dehydrogenase) 含有對大腸桿菌(E. coli)、銅綠假單胞菌(P. aeruginosa)和白色念珠菌(C. albicans)具有抗菌活性(antimicrobial activity)的肽。對小鼠中類似蛋白質的研究已經確定了多種附加功能，包括核蛋白的亞硝基化(nitrosylation)、mRNA 穩定性(RNA stability)的調節以及作為巨噬細胞(macrophage)細胞表面的轉鐵蛋白受體(transferrin receptor)。許多與該基因座相似的假基因(pseudogenes)存在於人類基因組(human genome)中。選擇性剪接(splicing)導致多個轉錄變體(ranscript variants)。
產品介紹: GAPDH多株抗體，免疫原Synthesized peptide derived from the N-terminal region of human GAPDH。通過使用特異性免疫原的親和層析從兔抗血清中親和純化抗體。辨視物種Human, Mouse, Rat。可應用於ELISA, IHC-P, WB實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000).。濃度1mg/ml，保存於PBS containing 50% Glycerol, 0.5% BSA and 0.05% Sodium Azide.
| Product name | GAPDH Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human GAPDH |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Alternative | GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.05% Sodium Azide. |
預染protein marker | 快速型蛋白質染色劑 | 活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 | Abbkine 細胞膜染色 | 活細胞Lysosome染色
Abbkine提供一系列高度特異性單克隆和多克隆抗體，tag antibody抗體系列商品如 His tag、GST tag、S tag、HA tag、DDDDK tag、Myc tag、MBP tag和 mCherry tag等單/多株Tag抗體。另具生物素(Biotin)、HRP、AbFluor™ 染料、瓊脂糖(agarose)和磁珠偶聯物(magnetic beads)標誌。
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