Calcein AM 活細胞螢光染劑 | 貨號1755-250 | 規格50ugX5

活細胞染劑 “Calcein AM”, 用於活細胞觀察及細胞侵入及轉移試劑；分子型式C₄₆H₄₆N₂O₂₃ | 分子量Molecular Weight: 994.86 | 純度Purity: ≥95% by HPLC | DMSO (5 mg/ml)。Calcein-AM是一種可對活細胞進行螢光標記的細胞染色試劑，Calcein-AM由於在Calcein(鈣黃綠素)的基礎上加強了疏水性，因此能夠輕易穿透活細胞膜。當其進入到細胞質後，酯酶會將其水解為Calcein(鈣黃綠素)留在細胞內,發出強綠色螢光。與其他同類試劑（如BCECF-AM和Carboxy-fluorescein diacetate）相比，Calcein-AM是最適合作為螢光探針去染活細胞的，因為它的細胞毒性很低。Calcein(鈣黃綠素)不會抑制任何的細胞功能如增殖或淋巴球的趨化性。另外，使用了Calcein(鈣黃綠素)的活性檢測的實驗結果是十分可信並且與標準的⁵¹Cr-釋放法所得結果相一致的。若需套組包裝請訂購K305 Calcein AM細胞活力檢測試劑

Calcein AM 活細胞螢光染劑 | 用於細胞活性分析之建議配製

Calcein AM分子量為995 g/mole , 稱取50ug的calcein AM可以溶在50ul的DMSO (1755-5為50ug包裝; 1755-250為5x50ug包裝)；最後濃度為1Mm取1ul的Calcein AM 溶在1X 的1000ul dissociation solution =1uM (1000ul)以96well 為例，加入100ul的1uM Calcein AM,大約可以使用500次。Calcein-AM的ester部位遇到濕氣會分解，使用後請在-20℃下密閉保存，溶於DMSO後建議2個月內使用完。試劑套組包裝請按此 Calcein AM細胞活力檢測試劑盒

Note: The final concentration of the Calcein AM will need to be empirically determined for different cell types and/or experimental conditions. Ranges of 1 – 10 M have been reported.

Calcein AM 活細胞螢光染劑 | 死活細胞雙重染色

Calcein(鈣黃綠素)的激發和發射波長分別為490 nm和515 nm。Calcein-AM和碘化丙啶（PI）溶液，它們分別可對活細胞和死細胞染色。Calcein-AM的乙酸甲基酯親脂性很高，使其可透過細胞膜，通過活細胞內的酯酶作用，Calcein-AM能脫去AM基，產生的Calcein(鈣黃綠素)能發出強綠色螢光，因此Calcein-AM僅對活細胞染色。另一方面，作為核染色染料的PI不能穿過活細胞的細胞膜。它穿過死細胞膜的無序區域而到達細胞核，並嵌入細胞的DNA雙螺旋從而產生紅色螢光（激發：535 nm，發射：617 nm），因此PI僅對死細胞染色。由於Calcein(鈣黃綠素)和PI-DNA都可被490 nm激發，因此可用螢光顯微鏡同時觀察活細胞和死細胞。用545 nm激發，僅可觀察到死細胞。根據以上特點，Calcein-AM和碘化丙啶（PI）經常被結合用來作為活細胞和死細胞的雙重染色。

BIOVISION and TREVIGEN™ Calcein AM Fluorescent Dye can be used to fluorescently label viable cells for a variety of assays such as tumor cell invasion, endothelial cell migration, and endothelial cell tubulogenesis.

Calcein AM 活細胞螢光染劑 | 相關商品

Catalog#	Product Name	Size
1755-1000	Calcein AM	1 mg
1755-50	Calcein AM	50 µg
1755-250	Calcein AM	5 x 50 µg
K305-1000	EZViable™ Calcein AM Cell Viability Assay Kit (Fluorometric)	1000 assays

 |更多Probe相關商品

Catalog #	Product Name+	Size
2752-100	EZSolution™ DAF-2	100 µg
2752-500	EZSolution™ DAF-2	500 µg
2752-1000	EZSolution™ DAF-2	1 mg
2753-100	EZSolution™ DAF-2 diacetate	100 µg
2753-500	EZSolution™ DAF-2 diacetate	500 µg
2753-1000	EZSolution™ DAF-2 diacetate	1 mg
2760-1	2-NBDG	1 mg
2760-5	2-NBDG

Biovison品牌	貨號	試劑品項	包裝	操作手冊下載
Biovision	1755	Calcein AM 活細胞螢光染劑 | 貨號1755-250 | 規格50ugX5	50ug/5x50ug/1mg
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Biovision	1692	Lovastatin| 降膽固醇藥物貨號1692-250  A HMG-CoA inhibitor	50mg/250mg
Biovision	K280	線粒體DNA分離套組 | 貨號K280-50	50 assays
Biovision	1691	Metformin, Hydrochloride | 貨號1691-5G   An AMPK activator	1g/5g/50g
Biovision	1649	IPTG, Animal-Free (Dioxane-Free) | 貨號1649-5G	1g/5g/50g
Biovision	1643	PD-0325901 | MEK選擇性非競爭型抑制劑| 貨號1643-25   A MEK inhibitor	2mg/5mg/25mg
Biovision	1568	Rapamycin免疫抑制劑 | 貨號1568-50 50mg	5mg/50mg
Biovision	1557	抗生素系列產品  | G418 sulfate | G418抗生素 | Antibiotic G418	1g/5g/25g
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Biovision	1545	高純度CHAPS 兩性離子非變性洗滌劑 | CAS#75621-03-3貨號1545	10g/50g/100g/500g/1kg
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Biovision	1218	anti-fading封片劑 FluorLast™ with DAPI and DABCO (FRASC) | 貨號1218-20	20ml
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Biovision	1211	“強效型” 螢光封片劑 SuperMOUNT™ Mounting Medium| 貨號1211-20	20ml
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Biovision	K787	乙醇脫氫酶測定試劑盒  | 貨號K787-100	100 assays
Biovision	K731	醛脫氫酶活性比色測定試劑盒 | 貨號K731-100	100 assays
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Biovision	K761	Glutathione Reductase Activity Colorimetric Assay Kit呈色分析套組 |貨號K761-200	200 assays
Biovision	K762	GPx呈色分析套組Glutathione Peroxidase Activity Colorimetric Assay Kit	100 assays
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Biovision	1010-100	Caspase-Family Inhibitor Z-VAD-FMK/ pan-caspase inhibitor | 貨號1010-100	100 µl (2 mM) Z-DEVD-FMK in DMSO
Biovision	K295	Cell Surface Protein Isolation Kit 細胞表面蛋白分離試劑盒 | 貨號K295	10 assays
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Biovision	K410	磷酸鹽比色測定試劑盒| 貨號K410-500	500 assays
Biovision	K630	(不同樣品來源) 游離甘油比色/螢光檢測試劑盒 | 貨號K630-100	100 assays
Biovision	K648	Glycogen Colorimetric Assay Kit II肝醣呈色分析套組 | 貨號K648-100  (適用於還原物質的樣品)	100 assays
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Biovision	k983	Aromatase (CYP19A) Activity Assay Kit (Fluorometric) 芳香酶螢光分析套組  | 貨號K983-100	100 assays
Biovision	k938	α-葡萄糖苷酶抑制劑篩選試劑盒 |α-Glucosidase Inhibitor Screening Kit (Colorimetric)貨號K938-100	100 assays
Biovision	k482	α-澱粉酶抑制劑篩選試劑盒|α-Amylase Inhibitor Screening Kit貨號 K482	100 assays
Biovision	6500B	Protein A (Lyophilized) 貨號6500B-10	10mg, 25mg, 100mg, 1g
Biovision	6510	重組蛋白G | Protein G 貨號6510	1mg, 5mg, 10mg,  100mg
Biovision	k936	活性氧（ROS）檢測試劑盒 | 貨號K936	100 / 250 assays
Biovision	k752	丙氨酸氨基轉移酶（ALT或SGPT）活性比色/螢光測定試劑盒 | 貨號K752-100	100 assays
Biovision	k627	乳酸比色測定試劑盒II Lactate Colorimetric Assay Kit II | 貨號K627-100  0.02 mM – 10 mM乳酸	100 assays
Biovision	k686	葡萄糖比色測定試劑盒II | 貨號K686-100  20μM – 10mM	100 assays
Biovision	2129	PBS Tablets | 貨號2129-10	10 Tablets, 100 Tablets
Biovision	1755	Calcein AM 活細胞螢光染劑 | 貨號1755-250 | 規格50ugX5	50ug, 250ug, 1000mg
Biovision	k654	Malate Dehydrogenase蘋果酸脫氫酶活性比色測定試劑盒 | 貨號K654-100	10 Tablets, 100 Tablets
Biovision	9210	分子等級重組蛋白酶K 100 mg | 貨號9210	100mg
Biovision	k2049	碳水化合物(Carbohydrates)檢測 |  貨號K2049-100	100 mg, 500 mg, 1 g, 10 g, 50 g
Biovision	k264	GSH/GSSG螢光分析套組Glutathione (GSH/GSSG/Total) Fluorometric Assay Kit    | 貨號K264-100	100 assays
Biovision	k306	BrdU細胞增殖測定試劑盒 | 貨號K306-1000	100 assays
Biovision	k795	單胺氧化酶活性（總MAO / MAO-A / MAO-B）螢光測定試劑盒| 貨號K795	100 assays
Biovision	1218	anti-fading封片劑 FluorLast™ with DAPI and DABCO (FRASC) | 貨號1218-20	20ml
Biovision	6288	Hemocyanin-Keyhole Limpet (KLH), Native | 貨號6288-25	25mg
Biovision	k818	BCA蛋白測定試劑盒 還原劑兼容 | 貨號K818-1000	1000 assays
Biovision	k4210	Kanamycin ELISA分析套組  | 貨號K4210-100	100 assays
Biovision	k1012	3.5kD DiaEasy™即用型透析管 | Dialyzer (3 ml) MWCO 3.5 kDa貨號K1012	10管, 25管, 100管
Biovision	k711	Amylase活性呈色分析套組 | 貨號K711-100 (alpha-amylase activity)	100 assays
Biovision	k771	胰蛋白酶活性測定試劑盒 | 貨號K771	100 assays
Biovision	k266	細胞核/細胞質分離試劑盒 Nuclear/Cytosol Fractionation Kit | 貨號K266-100	100 assays
Biovision	k748	Phosphatidic acid磷脂酸檢測試劑盒 | 貨號K748-100	100 assays
Biovision	k417	ATP酶活性測定試劑盒（呈色法）ATPase Activity Assay Kit (Colorimetric)  | 貨號K417-100	100 assays
Biovision	E4626	透明質酸ELISA檢測 | 貨號E4626-100	96 assays
Biovision	1073	Caspase-2 Inhibitor Z-VDVAD-FMK | 貨號1073-20C	20 µl (10 mM), 100 µl (2 mM)
Biovision	1001	Annexin V-FITC Reagent  | 貨號1001-200	200 assays / 1000 assays
Biovision	1042	Dexamethasone | CAS #50-02-2貨號1042	1g/10g/1ml
Biovision	1056	Propidium Iodide 死細胞核酸染劑 | 貨號1056	1ml
Biovision	1075	Caspase-9 Substrate Ac-LEHD-AFC | 貨號1075-200	200 assays / 1000 assays
Biovision	1083	Caspase-3 重組蛋白 | 貨號1083	25 units /100 units / 5 µg /10 µg
Biovision	1149	Z-LEHD-FMK | 貨號1149	1mg/5mg
Biovision	1035	1 X Annexin V Binding Buffer  | 貨號1035-100	100 ml
Biovision	1007	Caspase-3 Substrate DEVD-AFC | 貨號1007-200	200 assays / 1000 assays