【磷酸鹽比色測定】Phosphate Colorimetric Assay Kit Abcam (Biovision)貨號K410-500
磷酸鹽比色測定試劑盒使用ELISA微量滴定板(microtiter plates0 提供500次測定，或使用分光光度計1ml比色皿(cuvettes)進行100次測定。
Phosphate Colorimetric Assay Kit
磷酸鹽(Phosphate)是生物體系中最重要的無機離子(inorganic ions)之一。它在各種角色中扮演重要作用。最重要的作用之一是作為分子開關( molecular switch)，通過生物系統中各種蛋白激酶(protein kinases)和磷酸酶(phosphatases)的介導使酶活性(enzyme activity)開啟和關閉。磷酸鹽( Phosphate)在礦化過程(mineralization processes )中也非常重要，並且是由於高肥料使用區域的徑流而在淡水體中經常發現的藻類水華的主要刺激物。新設計的磷酸鹽比色分析試劑盒( Phosphate Colorimetric Assay Kit, k410) 提供了一個簡單，快速和靈敏的方法來評估各種濃度的磷酸鹽(Phosphate)。該測定法使用孔雀石綠( malachite green)和鉬酸銨(ammonium molybdate)的專有配方，其與磷酸根離子形成顯色複合物( chromogenic complex )，在650nm附近產生強烈的吸收帶。可以直接確定磷酸鹽濃度在1μM和1mM之間，檢測下限為約0.1nmol。磷酸鹽比色測定試劑盒使用ELISA微量滴定板(microtiter plates0 提供500次測定，或使用分光光度計1ml比色皿(cuvettes)進行100次測定。注意事項: 此商品使用1.0 ml比色皿測量時，可進行100次測定 (微量盤為500次分析)：使用1 ml比色皿測量時，需注意將所有反應(reaction components)增加5倍使用。 1 ml總反應混合物(total reaction mixture)將包含0-25 nmol磷酸鹽（0-500 µl），150 µl磷酸鹽試劑，並用蒸餾水補足至1.0 ml。在室溫下孵育30分鐘，然後在650 nm波長處讀數。使用1ml比色皿進行100次測定。
ELISA儀器設備請按此 (標配405、450、492、630 nm濾片/最高可擴充至7濾片）（k410 λ= 650 nm）
| Cat # +Size | K410-500 |
| Size | 500 assays |
| Detection Method | Absorbance (650 nm) |
| Species Reactivity | Mammalian |
| Applications | Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. |
| Features & Benefits | Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use. The newly designed Phosphate Colorimetric Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around 650 nm. Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. The Phosphate Colorimetric Assay Kit provides 500 assays using microtiter plates or 100 assays using 1 ml cuvettes. |
| Kit Components | • Phosphate Reagent • Phosphate Standard (10 mM) |
鈣呈色測定 | Magnesium鎂呈色測定 |磷酸鹽比色測定 | 鈉呈色分析 | Zinc鋅呈色測定
引用文獻
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Krieger et al., Effect of Potassium Citrate on Calcium Phosphate Stones in a Model of Hypercalciuria. J. Am. Soc. Nephrol., Apr 2015; 10.1681/ASN.2014121223.
Burris et al., Estrogen directly and specifically downregulates NaPi-IIa through the activation of both estrogen receptor isoforms (ERα and ERβ) in rat kidney proximal tubule. Am J Physiol Renal Physiol, Mar 2015; 308: F522 – F534.
Prazeres et al., Ocean acidification induces biochemical and morphological changes in the calcification process of large benthic foraminifera. Proc R Soc B, Mar 2015; 282: 20142782.
Wu-Wong et al., Vitamin D receptor agonist VS-105 improves cardiac function in the presence of enalapril in 5/6 nephrectomized rats. Am J Physiol Renal Physiol, Feb 2015; 308: F309 – F319.
