丙酮酸 (PA) 檢測試劑盒 | Pyruvate Acid (PA) Assay Kit KTB1121

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相關下載：	丙酮酸-PA-檢測試劑盒操作手冊

特點

丙酮酸 (PA) 檢測試劑盒 | Pyruvate Acid (PA) Assay Kit KTB1121 (偵測範圍: 1.094-70 µg/mL, 靈敏度0.5 µg/mL)

CheKine™ Micro Pyruvate Acid (PA) Assay Kit

丙酮酸（Pyruvate Acid, PA）是生物體內糖代謝和體內多種物質相互轉化的重要中間體。它透過乙醯輔酶A (acetyl CoA )連接葡萄糖、脂肪酸和胺基酸三大代謝，發揮重要的樞紐作用。該試劑盒提供了一種簡單的方法來檢測各種生物樣本中的丙酮酸 (Pyruvate Acid, PA) 濃度，例如動物組織(animal tissues)、植物組織(plant tissues)、細胞培養物(cell culture)：貼壁或懸浮細胞、血清、細菌。測定中，丙酮酸與2,4-二硝基苯肼(2,4-dinitrophenylhydrazine )反應生成丙酮酸-2,4-二硝基苯腙( pyruvate-2,4-dinitrophenylhydrazone)，在鹼性條件( alkaline conditions)下呈棕紅色，在520 nm處有特徵吸收峰。樣品中存在的丙酮酸 (Pyruvate Acid, PA) 與所得的訊號成正比。

The kit provides a simple method for detecting Pyruvate Acid (PA) concentration in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Pyruvic acid reacts with 2,4-dinitrophenylhydrazine to generate pyruvate-2,4-dinitrophenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 520 nm. The Pyruvate Acid (PA) present in the sample is proportional to the signal obtained.

Product name	CheKine™ Micro Pyruvate Acid (PA) Assay Kit
Applications notes	The kit provides a simple method for detecting Pyruvate Acid (PA) concentration in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Pyruvic acid reacts with 2,4-dinitrophenylhydrazine to generate pyruvate-2,4-dinitrophenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 520 nm. The Pyruvate Acid (PA) present in the sample is proportional to the signal obtained.
Alternative	PA; Pyruvic acid
Kit components	• Extraction Buffer • Chromogen A • Chromogen B • Standard
Features & Benefits	• Determination of Pyruvate Acid (PA) content in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. • Standard and standard curve line are provided.
Usage notes	• Do not mix or substitute reagents or materials from other kit lots or vendors. • The lowest detection limit is 1μg/mL or 1μg/g fresh weight or 10ng/mg prot. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions	Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Pyruvate Acid (PA) is an important intermediate for the sugar metabolism in organisms and the mutual transformation of various substances in the body. It connects the three major metabolisms of glucose, fatty acids and amino acids through acetyl CoA and plays an important pivotal role.
Alternative	PA; Pyruvic acid

代謝分析 / 生化檢測

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