CheKine™ 微量游離脂肪酸 (FFA) 檢測試劑盒| Free Fat Acid (FFA) Assay Kit Abbkine貨號KTB2230 

CheKine™ Micro Free Fat Acid (FFA) Assay Kit 偵測範圍0.0313-2mM 靈敏度0.0156mM

產品介紹: 血清中游離脂肪酸 ( Free Fat Acid, FFA) 濃度與脂質代謝(lipid metabolism)、碳水化合物代謝(carbohydrate metabolism)及內分泌功能(endocrine function)有關。糖尿病(diabetes)、嚴重肝功能障礙(severe liver dysfunction)、甲狀腺機能亢進(hyperthyroidism )等疾病會導致FFA游離脂肪酸濃度升高。CheKine™ Micro 游離脂肪酸 ( Free Fat Acid, FFA) 檢測試劑盒提供了一種檢測 游離脂肪酸 ( Free Fat Acid, FFA) 的便利工具。其原理是游離脂肪酸 ( Free Fat Acid, FFA) 與銅離子結合形成脂肪酸銅鹽，可溶於氯仿。用銅試劑法測定銅離子的含量即可計算出游離脂肪酸 ( Free Fat Acid, FFA) 的含量。

As FABP5 and FASN are genes involved in lipid metabolism, we measured intracellular free FAs and free cholesterol content in different QGP-1 cells. (from reference)

Product Properties

Kit components	• Cu Reagent • Chromogen • Standard（16.41 mg palmitic acid）
Features & Benefits	• Determine FFA content in plant serum (plasma), animal and plant tissues, cells, bacteria and other samples. • Detailed sample preparation and result calculation methods are provided.
Storage instructions	Store at 4℃ for 12 months away from light
Shipping	Blue ice
Precautions	The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes.

Additional Information

Background

MAO (EC1.4.3.4) mainly exists in various organs of vertebrates, especially secretory glands, brain and liver. It also exists in plants such as invertebrates and legumes. Although the content of MAO is low in vivo, it has important physiological functions and its activity can reflect the degree of liver fibrosis. In addition, the abnormal activity of MAO leads to the disorder of the monoamine neurotransmitter in the cell, which leads to a variety of diseases.

樣品製備(Sample Preparation)

注意事項：建議使用新鮮樣品。如果不立即分析，樣品可在-80℃保存6個月。

1.動物組織(Animal tissues)：秤取0.1g組織，加入1mL萃取緩衝液，勻漿，4℃，8,000rpm離心10分鐘，取上清進行測定。

2.植物組織(Plant tissues)：秤取0.1g組織，加入1mL萃取緩衝液，搗碎。超音波破碎5分鐘（功率20%或200W，超音波3秒，間隔7秒，重複30次）。4℃，8,000rpm離心10分鐘，取上清進行測定。

3.Cells or bacteria：收集5×106細胞吸附菌於離心管中，以冷PBS洗滌細胞吸附菌，離心後棄上清；加入1mL萃取緩衝液超音波破碎細胞吸附菌5分鐘（功率20%或200W ，超音波3秒，間隔7秒，重複30次）。8,000rpm離心10分鐘4℃，取上清液進行測定。

4.液體樣本如血清：直接測試。