鈣含量測定試劑盒 | CheKine™ Micro Calcium Content Assay Kit Abbkine貨號KTB1117

CheKine™ Micro Calcium Content Assay Kit

產品介紹:

鈣（calcium）通常是哺乳動物體內最豐富的礦物質，參與調節多種細胞生命過程。它也是最重要的細胞內調節因子之一。鈣（calcium）可以以兩種形式存在：游離離子或與鈣離子結合的複合物，例如磷酸鈣和碳酸鈣，它們是骨組織的組成部分。許多生理過程，包括肌肉收縮(muscle contraction)、細胞黏附(cell adhesion)、荷爾蒙/神經傳導物質釋放(hormone/neurotransmitter release)、肝醣代謝(glycogen metabolism)、細胞增殖/分化 (cell proliferation/differentiation)、血液凝固(blood coagulation)、神經(neural)或突觸傳遞(synaptic transmission)以及骨骼結構支撐(bone structure support)，都受鈣訊號調控(bone structure support)。細胞特異性鈣訊號系統完整性的缺陷可導致各種疾病的發生。儘管人體中99%的鈣存在於骨骼和牙齒中，但臨床關注點通常集中在血鈣水平，即血清鈣水平。血鈣濃度主要受副甲狀腺素的調節，副甲狀腺素控制骨骼中鈣的儲存或釋放，以防止高血鈣或低血鈣。血清中的鈣主要以三種形式存在：約50％為游離鈣離子，約45％為蛋白質結合鈣，約5％為鈣複合物（主要是檸檬酸鈣）。鈣離子水平通常與磷離子水平成反比。 CheKine™ 微量鈣離子含量測定試劑盒提供了一種簡單、方便、快速的鈣離子含量檢測方法，適用於動植物組織、細胞、血清（血漿）或其他液體樣本。其原理是基於鈣離子在鹼性條件下與鄰甲酚酞絡合酮反應(o-cresolphthalein complexone )生成紫色複合物。透過測量575 nm處的吸光度即可定量測定鈣離子含量。

Calcium (calcium) is typically the most abundant mineral in the bodies of mammals and is involved in regulating numerous cellular life processes. It is also one of the most important intracellular regulatory factors. Calcium can exist in two forms: as free ions or as complexes with bound calcium ions, such as calcium phosphate and calcium carbonate, which are components of bone tissue. A wide range of physiological processes, including muscle contraction, cell adhesion, hormone/neurotransmitter release, glycogen metabolism, cell proliferation/differentiation, blood coagulation, neural or synaptic transmission, and bone structure support, are regulated by calcium signaling. Defects in the integrity of cell-specific calcium signaling systems can lead to the development of various diseases. Although 99% of the calcium in the human body is found in bones and teeth, clinical attention is often focuse on blood calcium levels, known as serum calcium. Blood calcium levels are primarily regulated by parathyroid hormone, which controls the storage or release of calcium from bones to prevent hypercalcemia or hypocalcemia. Calcium in serum exists primarily in three forms: approximately 50% as free calcium ions, about 45% as protein-bound calcium, and about 5% as calcium complexes (mainly calcium citrate). Calcium levels are typically inversely related to phosphorus levels. CheKine™ Micro Calcium Content Assay Kit provides a simple, convenient, and rapid method for detecting calcium ion content, suitable for animal and plant tissues, cells, serum (plasma) or other Liquid samples. The principle is based on the reaction of calcium ions with o-cresolphthalein complexone under alkaline conditions to form a purple complex. The calcium ion content can be quantitatively determined by measuring the absorbance at 575 nm.

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注意：盡可能使用新鮮樣品。如不立即測定，樣品可在-80℃保存1個月。測定時，應控制解凍溫度和時間。室溫解凍時，樣品應在4小時內解凍。