肌酸酐（Cr）含量測定試劑盒 | CheKine™ Mirco Creatinine (Cr) Content Assay Kit Abbkine貨號KTB1002

CheKine™ Mirco Creatinine (Cr) Content Assay Kit

產品介紹:

肌酸酐(Creatinine)是人體肌肉代謝的副產物，主要經由腎臟的腎絲球濾過(glomerular filtration)排出。血液中肌酸酐(creatinine)的來源包括內源性(endogenous)和外源性(exogenous )成分。血清肌酸酐幾乎完全透過腎小球(glomeruli )過濾到原尿中，不會被腎小管(renal tubules)重新吸收。內生性肌酸酐的每日生成量幾乎保持恆定，當嚴格控制外源性肌酸酐的攝取量時，血清肌酸酐濃度可達一個穩定值。因此測定血清肌酸酐(serum creatinine)濃度可以反映腎小球的濾過功能。 CheKine™微量肌酸酐（Cr）含量測定試劑盒(CheKine™ Mirco Creatinine (Cr) Content Assay Kit) 提供了一種簡單、方便、快速的檢測肌酸酐含量的方法，適用於血清、血漿、尿液等液體樣本。本測定原理是基於肌酸酐水解酶將肌酸酐水解為肌酸。然後，肌酸在肌酸酐醯胺水解酶(creatinine amidohydrolase)的作用下轉化為肌氨酸(sarcosine)和尿素( urea)。其中的產物肌氨酸(Sarcosine)被肌氨酸氧化酶(sarcosine oxidase)氧化，生成肌氨酸(sarcosine)、過氧化氫(hydrogen peroxide)和甲醛(formaldehyde)。最後，過氧化氫( hydrogen peroxide)在過氧化物酶的催化下與髮色底物4-氨基安替比林(chromogenic substrates 4-aminoantipyrine)和N-乙基-N-磺丙基-間甲苯胺(N-ethyl-N-sulfopropyl-m-toluidine )反應，生成紅色的奎尼胺化合物(quinimine compound)。由於奎尼胺(quinimine)在波長546nm處有最大吸收峰，因此在一定的濃度範圍內，546nm處的吸光度變化與樣本中的肌酸酐含量成正比。

樣品製備: 

建議您使用新鮮樣品。若不立即測定，樣本可在-80℃保存一個月。 1.血清（血漿）：直接檢測。 2.尿液：建議檢測前以生理食鹽水稀釋2-10倍，將結果乘以稀釋倍數

Product name	CheKine™ Mirco Creatinine (Cr) Content Assay Kit
SampleType	Serum, Plasma, Urine, and other liquid samples
Alternative	Creatinine(Cr)
Kit components	• Reagent I •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •Reagent Ⅴ •Reagent Ⅵ •Reagent Ⅶ •Standard
Features & Benefits	The verified samples are complete in type and easy to operate．
Usage notes	• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If  don’t perform the assay at the same time, it’s better to complete the Sample Preparation step before storing the samples.
Storage instructions	Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Creatinine is a metabolic byproduct of muscle metabolism in the human body and is primarily excreted through glomerular filtration by the kidneys. The sources of creatinine in the blood include both endogenous and exogenous components. Serum creatinine is almost entirely filtered through the glomeruli into the primary urine and is not reabsorbed by the renal tubules. The daily production of endogenous creatinine remains almost constant, and when the intake of exogenous creatinine is strictly controlled, the serum creatinine concentration reaches a stable value. Therefore, measuring the serum creatinine concentration can reflect the glomerular filtration function.CheKine™ Mirco Creatinine (Cr) Content Assay Kit provides a simple, convenient, and rapid method for detecting creatinine content, suitable for liquid samples such as serum, plasma, urine, etc. The principle of this assay is based on the hydrolysis of creatinine to creatine by creatinine amidohydrolase. Creatine is then converted to sarcosine and urea by creatine amidinohydrolase. Sarcosine, one of the products, is oxidized by sarcosine oxidase to generate sarcosine, hydrogen peroxide, and formaldehyde. Finally, the hydrogen peroxide reacts with chromogenic substrates 4-aminoantipyrine and N-ethyl-N-sulfopropyl-m-toluidine under the catalysis of peroxidase to produce a red quinimine compound. Since quinimine has a maximum absorption peak at a wavelength of 546 nm, within a certain concentration range, the change in absorbance at 546 nm is directly proportional to the creatinine content in the sample.
Alternative	Creatinine(Cr)