6-磷酸果糖激酶 (PFK) 活性檢測試劑盒 | 6-Phosphofructokinase(PFK) Activity Assay Kit貨號KTB1124

(96-well UV plateor microquartz cuvette)

產品描述：磷酸果糖激酶（Phosphofructokinase , PFK）廣泛存在於動物、植物、微生物和培養細胞中。它負責將果糖 6-磷酸(fructosae 6-phosphate)和 ATP 轉化為果糖 1,6 二磷酸(fructosae 1,6 diphosphate )和 ADP。它是糖酵(glycolysis )解過程中的關鍵調節酶之一。細菌細胞上清液(bacterial cell supernatant )和其他樣品中的磷酸果糖激酶 (PFK) 活性。CheKine™ Micro 6-磷酸果糖激酶（CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit, ）活性檢測試劑盒提供了一種簡單、方便、快速的比色法檢測 PFK 的方法。 PFK催化果糖6-磷酸( fructosetose 6-phosphate)和ATP生成果糖1、6-二磷酸( fructose-1, 6-diphosphate)和ADP，丙酮酸激酶(pyruvate kinase)和乳酸脫氫酶(lactate dehydrogenase)依次進一步催化NADH氧化為NAD+。在340nm處測量NADH下降率，反映PFK活性。

Product name	CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit
Applications notes	CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric PFK detection method. PFK catalyzed fructosetose 6-phosphate and ATP to generate fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyzed NADH oxidation to NAD+ in turn. The NADH decline rate was measured at 340nm, which reflected the PFK activity
Kit components	•Extraction Buffer • Assay Buffer • Substrate Mix • Enzyme 1 • Enzyme 2
Features & Benefits	• Detect PFK activity in bacterial supernatant of serum (plasma) animal and plant tissues/cells • Detailed sample preparation and results calculation methods are provided
Usage notes	• Do not mix or substitute reagents or materials from other kit lots or vendors. • It is advisable to measure serum potassium as soon as possible after blood collection. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions	Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping	Gel pack with blue ice.