CheKine™ 乳酸比色測定試劑盒 | CheKine™ Lactate Colorimetric Assay Kit貨號KTB1100
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特點
CheKine™ 乳酸比色測定試劑盒 | CheKine™ Lactate Colorimetric Assay Kit KTB1100 (靈敏度0.03 mM-2 mM)
檢測生物樣品中的 L (+) -Lactate，例如血清(serum)或血漿(plasma)、細胞(cells)、培養基(culture)和發酵培養基(fermentation media)中的 L (+) -Lactate。
乳酸（CH 3 CH（OH）COO-）在許多生物過程中起重要作用。 異常高濃度的乳酸與疾病狀態如糖尿病和乳酸酸中毒等有關.L（+） – 乳酸鹽是人體中間代謝中形成的主要乳酸鹽立體異構體，存在於血液中。 D（ – ） – 乳酸鹽也存在，但僅在L（+） – 乳酸鹽濃度的約1-5％。 在乳酸鹽測定試劑盒中，乳酸被乳酸脫氫酶(lactate dehydrogenase)氧化生成產物，該產物與四唑鎓鹽 WST-8 染料(tetrazolium salt WST-8 dye)相互作用形成比色 (450 nm) 產物，與存在的乳酸(lactate)成比例。試劑需配合ELISA儀器使用，儀器請按此連結。CheKine™ Lactate Assay Kit 提供了一種方便的方法來檢測生物樣品中的 L (+) -Lactate，例如血清(serum)或血漿(plasma)、細胞(cells)、培養基(culture)和發酵培養基(fermentation media)中的 L (+) -Lactate。 不需要預處理或純化樣品。 它在各種樣品中檢測到0.03 mM-2 mM乳酸。操作手冊 CheKine™ Lactate Assay Kit
Fig. Standard Curve of Lactate assay. The y-axis is OD450 and the x-axis is Lactate concentration (mM).
| Kit components | • Lactate Assay Buffer • Lactate Dehydrogenase • Lactate Dehydrogenase Cofactor • WST-8 • Enhancer • L (+) -Lactate Standard (100 mM) |
| Features & Benefits | • Determination of L (+) -Lactate in serum, plasma, cell/tissue samples, plant tissues,urine and other biological fluids. • Detect biological samples at a level of 0.03 mM-2 mM. • Pre-treatment or purification of samples are not required. • Using WST-8 as substrate, more sensitive than MTT. |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month. • It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range. • Fresh samples are necessary for good results. If don’t perform the assay at the same time, it’s better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
(文獻引用出自)
From: Peptide-based PROTAC degrader of FOXM1 suppresses cancer and decreases GLUT1 and PD-L1 expression
| Kit components | • Lactate Assay Buffer • Lactate Dehydrogenase • Lactate Dehydrogenase Cofactor • WST-8 • Enhancer • L (+) -Lactate Standard (100 mM) |
| Features & Benefits | • Determination of L (+) -Lactate in serum, plasma, cell/tissue samples, plant tissues,urine and other biological fluids. • Detect biological samples at a level of 0.03 mM-2 mM. • Pre-treatment or purification of samples are not required. • Using WST-8 as substrate, more sensitive than MTT. |
| Product name | CheKine™ Micro Lactate Assay Kit |
| Applications notes | CheKine™ Micro Lactate Assay Kit provides a convenient means for detecting L (+) -Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. In this kit lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a tetrazolium salt WST-8 dye to form a colorimetric (450 nm) product, proportional to the lactate present. |
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| KTB1011 | CheKine™ Micro Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit |
| KTB1020 | CheKine™ Micro Coenzyme Ⅰ NAD(H) Assay Kit |
| KTB1022 | CheKine™ Micro NAD+ kinase (NADK) Activity Assay Kit |
| KTB1030 | CheKine™ Micro Superoxide Dismutases (SOD) Activity Assay Kit |
| KTB1040 | CheKine™ Micro Catalase (CAT) Activity Assay Kit |
| KTB1050 | CheKine™ Micro Lipid Peroxidation (MDA) Assay kit |
| KTB1070 | CheKine™ Micro Xanthine Oxidase Activity Assay Kit |
| KTB1080 | CheKine™ Micro Superoxide anion Scavenging Capacity Assay Kit |
| KTB1100 | CheKine™ Micro Lactate Assay Kit |
| KTB1110 | CheKine™ Micro Lactate Dehydrogenase (LDH) Assay Kit |
| KTB1300 | CheKine™ Micro Glucose Assay Kit |
| KTB1310 | CheKine™ Micro Glucose Oxidase Activity (GOD) Assay Kit |
| KTB1041 | CheKine™ Micro Hydrogen Peroxide (H2O2) Assay Kit |
| KTB1400 | CheKine™ Micro Nitric Oxide (NO) Assay Kit |
| KTB1500 | CheKine™ Micro Total Antioxidant Capacity (TAC) Assay Kit |
| KTB1600 | CheKine™ Micro Reduced Glutathione (GSH) Assay Kit |
| KTB1610 | CheKine™ Micro Glutathione Oxidized (GSSG) Assay Kit |
| KTB1620 | CheKine™ Micro Glutathione Reductases (GR) Activity Assay Kit |
| KTB1630 | CheKine™ Micro Glutathione S-Transferase (GST) Assay Kit |
| KTB1640 | CheKine™ Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit |
| KTB1650 | CheKine™ Micro Thioredoxin Reductase (TrxR) Assay Kit |
| KTB1660 | CheKine™ Micro Thioredoxin Peroxidase (TPX) Assay Kit |
| KTB1021 | CheKine™ Micro NADH Oxidase (NOX) Assay Kit |
| KTB1120 | CheKine™ Micro Pyruvate Kinase (PK) Assay Kit |
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| KTB1700 | CheKine™ Micro Tissue and Blood Alkaline Phosphatase (AKP/ALP) Activity Assay Kit |
| KTB1800 | CheKine™ Micro Na⁺/K⁺-ATPase Activity Assay Kit |
| KTB1810 | CheKine™ Micro Ca2+/Mg2+-ATPase Activity Assay Kit |
| KTB1900 | CheKine™ Micro Monoamine Oxidase (MAO) Activity Assay Kit |
| KTB1140 | CheKine™ Micro Polyphenol Oxidase (PPO) Activity Assay Kit |
| KTB2100 | CheKine™ Micro Serum Potassium (K+) Assay Kit |
| KTB2200 | CheKine™ Micro Triglyceride (TG) Assay Kit |
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| KTB2220 | CheKine™ Micro Total Cholesterol (TC) Assay Kit |
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| KTB1410 | CheKine™ Micro Alanine Aminotransferase (ALT/GPT) Activity Assay Kit |
| KTB1420 | CheKine™ Micro Aspartate Aminotransferase (AST/GOT) Activity Assay Kit |
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| KTB1122 | CheKine™ Micro Phosphoenolpyruvate Carboxylase (PEPC) Activity Assay Kit |
| KTB1670 | CheKine™ Micro Toal Glutathione (T-GSH) Assay Kit |
| KTB1450 | CheKine™ Micro Cysteine (Cys) Assay Kit |
| KTB1160 | CheKine™ Micro Phenylalanine Ammonia Lyase (PAL) Activity Assay Kit |
| KTB1430 | CheKine™ Micro Proline (PRO) Assay Kit |
| KTB1440 | CheKine™ Micro Glutamate (Glu) Assay Kit |
| KTB1680 | CheKine™ Micro γ-Glutamyl Cysteine Ligase (GCL) Activity Assay Kit |
| KTB1690 | CheKine™ Micro Gamma-Glutamyl Transpeptidase (GGT) Activity Assay Kit |
| KTB1150 | CheKine™ Micro Peroxidase (POD) Activity Assay Kit |
| KTB1320 | CheKine™ Micro Plant Soluble Sugar Assay Kit |
| KTB1530 | CheKine™ Micro Plant Flavonoids Assay Kit |
| KTB1520 | CheKine™ Micro Plant Oligomeric Proantho Cyanidins (OPC) Assay Kit |
| KTB1540 | CheKine™ Micro Plant Total Phenols (TP) Assay Kit |
| KTB1210 | CheKine™ Micro Superoxide Anion Assay Kit |
| KTB2130 | CheKine™ Micro Serum Sodium Assay Kit |
| KTB2140 | CheKine™ Micro Serum Zinc Assay Kit |
| KTB1850 | CheKine™ Micro Mitochondrial complex I Activity Assay Kit |
| KTB1860 | CheKine™ Micro Mitochondrial complexⅡ Activity Assay Kit |
| KTB1870 | CheKine™ Micro Mitochondrial complex Ⅲ Activity Assay Kit |
| KTB1880 | CheKine™ Micro Mitochondrial complex Ⅳ Activity Assay Kit |
| KTB1890 | CheKine™ Micro Mitochondrial complex Ⅴ Activity Assay Kit |
| KTB1023 | CheKine™ Micro Citrate Synthase (CS) Activity Assay Kit |
| KTB2150 | CheKine™ Micro Total Iron-Binding Capacity (TIBC) Assay Kit |
| KTB1270 | CheKine™ Micro Pyruvate Dehydrogenase (PDH) Activity Assay Kit |
| KTB1910 | CheKine™ Reactive Oxygen Species (ROS) Detection Fluorometric Assay Kit |
| KTB1260 | CheKine™ Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit |
| KTB1220 | CheKine™ Micro Diamine Oxidase (DAO) Activity Assay Kit |
| KTB1230 | CheKine™ Micro Succinate Dehydrogenase（SDH) Activity Assay Kit |
| KTB1240 | CheKine™ Micro α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit |
| KTB1250 | CheKine™ Micro Mitochondrial Isocitrate Dehydrogenase（ICDHm）Activity Assay Kit |
| KTB1330 | CheKine™ Micro Blood Glucose Assay Kit |
| KTB1340 | CheKine™ Micro Glycogen Assay Kit |
| KTB1350 | CheKine™ Micro Total Carbohydrate Assay Kit |
| KTB1360 | CheKine™ Micro Reducing Sugar(RS) Assay Kit |
| KTB1550 | CheKine™ Micro Ceruloplasmin Activity Assay Kit |
| KTB1060 | CheKine™ Micro Advanced Oxidation Protein Products (AOPP) Assay Kit |
| KTB1091 | CheKine™ Micro Hydroxyl Free Radical Scavenging Capacity Assay Kit |
| KTB1123 | CheKine™ Micro Hexokinase (HK) Activity Assay Kit |
| KTB1124 | CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit |
| KTB1460 | CheKine™ Micro Amino Acid (AA) Assay Kit |
| KTB1125 | CheKine™ Micro Pyruvate Decarboxylase (PDC) Assay Kit |
| KTB1480 | CheKine™ Micro Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Assay Kit |
| KTB1470 | CheKine™ Micro Lysine (LYS) Assay Kit |
| KTB2230 | CheKine™ Micro Free Fat Acid (FFA) Assay Kit |
| KTB1370 | CheKine™ Micro α-Amylase Activity Assay Kit |
| KTB1380 | CheKine™ Micro β-Amylase Activity Assay Kit |
| KTB1390 | CheKine™ Micro Starch Branching Enzyme(SBE) Activity Assay Kit |
| KTB1710 | CheKine™ Micro Acetylcholinesterase (AchE) Activity Assay Kit |
| KTB1501 | CheKine™ Oxygen Radical Antioxidant Capacity (ORAC) Fluorometric Assay Kit |
| KTB1502 | CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit |
| KTB1560 | CheKine™ Micro Alcohol Acyltransferase（AAT）Activity Assay Kit |
| KTB2240 | CheKine™ Micro Fatty Acid Synthetase(FAS) Activity Assay Kit |
| KTB1290 | CheKine™ Micro Aconitase (ACO) Activity Assay Kit |
| KTB1280 | CheKine™ Micro Mitochondrial Malate Dehydrogenase(mMDH) Activity Assay Kit |
| KTB2260 | CheKine™ Micro Low Density Lipoprotein Cholesterol (LDL-C) Assay Kit |
| KTB2250 | CheKine™ Micro High Density Lipoprotein Cholesterol (HDL-C) Assay Kit |
| KTB1541 | CheKine™ Micro Tannin Assay Kit |
| KTB1542 | CheKine™ Micro Tannase(TAN) Activity Assay Kit |
| KTB1012 | CheKine™ Micro Creatine Kinase (CK) Activity Assay Kit |
| KTB1013 | CheKine™ Micro 6-Phosphogluconate Dehydrogenase Activity Assay Kit |
| KTB3020 | CheKine™ Micro NADP-Malate Dehydrogenase（NADP-MDH）Activity Assay Kit |
| KTB3021 | CheKine™ Micro NAD-Malate Dehydrogenase（NAD-MDH）Activity Assay Kit |
| KTB3030 | CheKine™ Micro Alcohol Dehydrogenase (ADH) Activity Assay Kit |
| KTB3040 | CheKine™ Micro Glutamate Synthase（GOGAT）Assay Kit |
| KTB3041 | CheKine™ Micro Glutamic Acid Dehydrogenase（GDH）Assay Kit |
| KTB3050 | CheKine™ Micro Water and Soil Nitrite Content Assay Kit |
| KTB3060 | CheKine™ Micro Sorbitol Dehydrogenase Assay Kit |
| KTB3051 | CheKine™ Micro Food Nitrite Assay Kit |
| KTB3070 | CheKine™ Micro Urease Activity Assay Kit |
| KTB1126 | CheKine™ Micro Phosphoenolpyruvate Carboxykinase（PEPCK) Activity Assay Kit |
| KTB4011 | CheKine™ Micro Soil Phosphate(S-PHOS) Assay Kit |
| KTB4020 | CheKine™ Micro Soil Leucine Arylamidase(S-LAP) Activity Assay Kit |
| KTB2160 | CheKine™ Micro Blood Phosphate Assay Kit |
| KTB2170 | CheKine™ Micro Tissue Inorganic Phosphorus Assay Kit |
| KTB4010 | CheKine™ Micro Soil Nitrate Reductase (S-NR) Activity Assay Kit |
| KTB3080 | CheKine™ Micro Plant Nitrate Nitrogen Assay Kit |
| KTB1014 | CheKine™ Micro Glucose-6-Phosphatase (G6P) Activity Assay Kit |
| KTB3090 | CheKine™ Micro Dehydroascorbic Acid (DHA) Assay Kit |
| KTB3091 | CheKine™ Micro Ascorbate Peroxidase (APX) Activity Assay Kit |
| KTB4012 | CheKine™ Micro Soil Nitrate Nitrogen Assay Kit |
| KTB4013 | CheKine™ Micro Neutral/Alkaline Soil Available Phosphorous Assay Kit |
| KTB4030 | CheKine™ Micro Soil Amylase(S-AL) Activity Assay Kit |
| KTB3092 | CheKine™ Micro Ascorbic Acid(AsA) Assay Kit |
| KTB3093 | CheKine™ Micro Ascorbic Acid Oxidase(AAO) Activity Assay Kit |
| KTB4021 | CheKine™ Micro Leucine Arylamidase(LAP) Activity Assay Kit |
| KTB4014 | CheKine™ Micro Acid Soil Available Phosphorous Assay Kit |
| KTB4015 | CheKine™ Micro Soil Ammonium Nitrogen Assay Kit |
| KTB3081 | CheKine™ Micro Plant Ammonium Nitrogen Assay Kit |
| KTB4040 | CheKine™ Micro Soil Acid Phosphatase(S-ACP) Activity Assay Kit |
| KTB4041 | CheKine™ Micro Soil Alkaline Phosphatase(S-AKP/ALP) Activity Assay Kit |
| KTB4050 | CheKine™ Micro Soil Catalase(S-CAT) Activity Assay Kit |