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β-半乳糖苷酶 (β-GAL) 活性檢測試劑盒| β-galactosidase (β-GAL) Activity Assay Kit Abbkine貨號KTB1324 

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β-半乳糖苷酶操作手冊

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β-半乳糖苷酶 (β-GAL) 活性檢測試劑盒| β-galactosidase (β-GAL) Activity Assay Kit Abbkine貨號KTB1324 

CheKine™ Micro β-galactosidase (β-GAL) Activity Assay Kit 偵測範圍0.0313-2mM 靈敏度0.0156mM

產品介紹: β-半乳糖苷酶(β-GAL,EC 3.2.1.23)是一種廣泛存在於動物、植物、微生物和培養細胞中的水解酶。催化β-半乳糖苷酶組合物中β-半乳糖苷酶鍵( β-galactosidase)的水解。除了具有反式半乳苷。 β-GAL不僅能釋放儲存的能量供植物快速生長,還能透過正常的生長釋放出游離的半乳糖。多醣代謝(normal polysaccharide metabolism)、細胞壁成分代謝(cell wall component metabolism)、多醣體(polysaccharides)、醣蛋白(glycoproteins)及末端的水解老化過程中細胞壁降解過程中的半乳糖殘留(galactose residues )。 β-GAL 可分解對硝基苯-β-D-吡喃半乳糖苷(p-nitrobenzene-β-D-galactopyranoside)生成對硝基苯酚(p-nitrophenol),在400 nm處有最大吸收峰,透過測量增加率來計算吸光度值 β-GAL 活性。β-半乳糖苷酶 (β-GAL) 活性檢測試劑盒( β-galactosidase (β-GAL) Activity Assay Kit Abbkine, KTB1324 )提供簡單、靈敏、快速的動物組織、植物組織中β-半乳糖苷酶(β-GAL) 檢測方法。及詳細的樣品製備和結果計算方法。

Product name CheKine™ Micro β-galactosidase (β-GAL) Activity Assay Kit
Applications notes •It is recommended to use fresh samples, which can be stored at -80 ° C for several weeks if the experiment is not carried out immediately. When measuring, the temperature and time of thawing should be controlled. When thawing at room temperature, the sample should be thawed within 4 h.
Kit components •Extraction Buffer
•Reagent I
•Reagent Il
•Reagent Ill
•Standard
Features & Benefits • Provides a simple, sensitive, rapid β-galactosidase (β-GAL) detection method in Animal Tissues, Plant Tissues..
• Detailed sample preparation and result calculation methods are provided.
Usage notes •It is recommended to use fresh samples, which can be stored at -80 ° C for several weeks if the experiment is not carried out immediately. When measuring, the temperature and time of thawing should be controlled. When thawing at room temperature, the sample should be thawed within 4 h.
Storage instructions Please store the whole kit at -20°C upon receipt ,and protect from light. The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background β-galactosidase (β-GAL, EC 3.2.1.23) is a hydrolytic enzyme that widely exists in animals, plants, microorganisms and cultured cells. It catalyzes the hydrolysis of β-galactosidase bonds in β-galactosidase compounds and also has the function of transgalactoside.

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