單胺氧化酶 (MAO) 活性檢測試劑盒| Monoamine Oxidase (MAO) Activity Assay Kit Abbkine貨號KTB1900 

CheKine™ Micro Monoamine Oxidase (MAO) Activity Assay Kit

產品介紹: MAO（EC1.4.3.4）主要存在於脊椎動物(vertebrates)的各個器官中，特別是分泌腺(secretory glands)、腦(brain)和肝臟(liver)。它也存在於無脊椎動物(invertebrates)和豆類(legumes)等植物中。 MAO在體內含量雖低，但具有重要的生理功能，其活性可反映肝纖維化(liver fibrosis)程度。此外，MAO活性異常導致細胞內單胺類神經傳導物質(monoamine neurotransmitter)紊亂，進而引發多種疾病。單胺氧化酶 (MAO) 活性檢測試劑盒 (KTB1900) 該試劑盒提供了一種簡單的方法來檢測血清(serum)、血漿(plasma)、組織(tissues)、細胞(cells)、細菌(bacteria)、植物(plants)和真菌(Fungi)等多種生物樣本中的 MAO 活性(MAO activity )。在測定中，MAO 催化單胺底物(monoamine substrates )脫氨基生成相應的醛(aldehydes)，醛(aldehydes)進一步氧化成酸(acid)。此基材在360 nm處有特徵吸收峰。單胺底物在 360 nm 處下降的速率可以反映 MAO 活性(MAO activity)。

Product Properties

Kit components	• Extraction Buffer I • Extraction Buffer II • Assay Buffer • Substrate
Features & Benefits	• Determination of MAO activity in a variety of biological samples such as serum, plasma, tissues, cells, bacteria, plants and Fungi. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.
Usage notes	• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions	Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background

MAO (EC1.4.3.4) mainly exists in various organs of vertebrates, especially secretory glands, brain and liver. It also exists in plants such as invertebrates and legumes. Although the content of MAO is low in vivo, it has important physiological functions and its activity can reflect the degree of liver fibrosis. In addition, the abnormal activity of MAO leads to the disorder of the monoamine neurotransmitter in the cell, which leads to a variety of diseases.

二抗(secondary antibody)是指用於靶向結合一抗的抗體。Abbkine 二抗可與酵素(HRP)、生物素(Biotin)或螢光團偶聯(DyLight)，用於各種基於抗體的應用，包括蛋白質印跡、免疫組織化學、免疫螢光、流式細胞術和 ELISA。，二抗( secondary antibody)和一抗聯合使用，檢測目的蛋白(target protein)，增強檢測信號(detection signal)。我們為您的每種應用提供來自山羊、兔和驢的高質量二抗。也可提供血清吸附二抗，推薦用於富含免疫球蛋白的樣品。Abbkine提供的二抗產品包括常用的酶標二抗（如HRP標記二抗）、DyLight螢光二抗、IFKine™二抗（取自驢，經其他物种血清吸附，具有較低的非-特異性結合和背景。特別適用於免疫學多標記實驗），IPKine™二抗（有效避免重鍊或輕鏈干擾，特別適用於IP-WB檢測）。