CheKine™ 微量肌酸激酶 (CK) 活性檢測試劑 | Micro Creatine Kinase (CK) Activity Assay Kit貨號KTB1012

肌酸激酶（Creatine Kinase , CK）（EC 2.7.3.2）主要存在於心臟(heart)、肌肉(muscle)、腦(brain)等組織中。它可以可逆地催化肌酸(creatine)和ATP之間的磷酸化反應(phosphorylation reaction)。它在能量運行(Energy operation)、肌肉收縮(muscle contraction )ATP再生中起重要作用，是臨床診斷心腦疾病的重要指標。 CK在各種組織和細胞類型中廣泛表達，在橫紋肌(striated muscles)，心臟組織(heart tissue)和腦(brain)中具有最高活性。 CK由兩個亞基組成：M（(muscle)肌肉）和B（(brain)腦），並且具有三種同工酶：CK-MM（(skeleton muscle)骨骼肌），CK-MB（(cardiac muscle)心肌）和CK-BB（CK-BB (brain)腦）。 CK水平升高與許多疾病相關，例如心肌梗塞(myocardial infarction)，肌營養不良(muscular dystrophy)，肺梗塞(pulmonary infarction)和腦腫瘤(brain tumors)。準確測量CK對早期診斷，預測和治療策略至關重要。CheKine™微量肌酸激酶（CheKine™Micro CreatineKinase(CK) Activity Assay Kit, KTB1012）活性測定試劑盒提供了一種簡單、方便、快速的CK活性檢測方法，適用於動物組織、細胞、血清等樣品的檢測。其原理是肌酸激酶（Creatine Kinase , CK）催化磷酸肌酸(creatine phosphate)和ADP生成肌酸和ATP，己糖激酶(hexokinase)催化ATP和葡萄糖形成6-磷酸葡萄糖( 6-phosphate glucose)，6-磷酸葡萄糖脫氫酶(6-phosphateglucosedehydrogenase)催化6-磷酸葡萄糖( 6-phosphate glucose)和NADP+生成NADPH，在340nm處有一個特徵吸收峰。 340 nm處NADPH增加的速率可以反映CK活性。

Product name	CheKine™ Micro Creatine Kinase (CK) Activity Assay Kit
Applications notes	Creatine Kinase (CK) (EC 2.7.3.2) mainly exists in the heart, muscle, brain and other tissues. It can reversibly catalyze the phosphorylation reaction between creatine and ATP. It plays an important role in energy operation, muscle contraction and ATP regeneration, and it is an important indicator for clinical diagnosis of heart and brain diseases.
Kit components	•Extraction Buffer •ReagentⅠ •ReagentⅡ
Features & Benefits	• Determination of animal tissues, cells, serum and other samples. • Detailed sample preparation and result calculation methods are provided.
Usage notes	• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors.
Storage instructions	Storage at -20°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Shipping	Gel pack with blue ice.

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