一氧化氮 (nitric oxide, NO)螢光探針-DAF-FM DA | CAS# 254109-22-3 MCE貨號 HY-D0717
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MCE (MedChem Express) Fluorescent Dye -DAF-FM DA
MCE (MedChemexpress) 總部位於美國新澤西，提供種類齊全的化學及生化研究用產品，包括創新的生命科學試劑，reference compounds (標準化合物)，APIs (原料藥) 及Natural compounds (天然化合物)以提供實驗室或科學研究之用，MedChemExpress(MCE)產品50000+ 特異性抑制劑、化合物庫、重組蛋白，專注於信號通路和疾病研究領域。全球文獻廣泛引用，質控體系嚴格，品質100%。官網連結 https://www.medchemexpress.com
DAF-FM DA | CAS# 254109-22-3 MCE貨號 HY-D0717
DAF-FM DA (Synonyms: Diaminofluorescein-FM diacetate)
產品介紹: MCE品牌提供的一系列化合物，DAF-FM DA (Diaminofluorescein-FM diacetate)（二氨基螢光素-FM二乙酸酯）是一種用於檢測和生物成像一氧化氮（nitric oxide, NO）的螢光探針。 DAF-FM DA可自發性穿過質膜，隨後被酯酶裂解生成細胞內DAF-FM（激發/發射495/515 nm)。
For research use only. We do not sell to patients.
DAF-FM DA Chemical Structure
CAS No. : 254109-22-3
| Description |
DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm)[1]. |
|---|---|
| In Vitro | Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs). 1.1 Preparation of stock solution[2] Use anhydrous DMSO to prepare a 5 mM DAF-FM DA stock solution. Note: It is recommended that the DAF-FM DA stock solution be stored at -20℃ or -80℃ in the dark after aliquoting. 1.2 Preparation of working solution Dilute the stock solution with Hank’s buffer or serum-free medium to prepare a 5 μM DAF-FM DA working solution. Note: Please adjust the concentration of DAF-FM DA working solution according to actual conditions and prepare it before use. 2 Staining steps 1) Seed breast cancer cells in a 6-well plate. 2) Aspirate the culture medium. 3) Add 5 μM DAF-FM DA working solution to each well and incubate at 37°C in the dark for 30 min to allow the probe to enter the cells. 4) Replace with fresh Hank’s buffer and continue incubation for 20 min to complete the deesterification reaction. 5) Counterstain the cell nucleus with Hoechst dye. 6) Quantify the fluorescence intensity using a microplate reader at 488 nm excitation and 515 nm emission, and normalize to the number of cells stained with Hoechst.MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
| In Vivo |
Using sections of zebrafish embryos live-stained with DAF-FM DA (5 μM), could confirm that the fluorescent signals were predominantly located in areas of ongoing bone formation[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
| Molecular Weight |
496.42 |
| Formula |
C25H18F2N2O7 |
| CAS No. | |
| Appearance |
Solid |
| Color |
Off-white to yellow |
| Emission (Em) |
515 |
| Excitation (Ex) |
495 |
MedChemexpress (MCE) 產品
MCE (MedChemexpress) 總部位於美國新澤西，提供種類齊全的化學及生化研究用產品，包括創新的生命科學試劑，reference compounds (標準化合物)，APIs (原料藥) 及Natural compounds (天然化合物)以提供實驗室或科學研究之用，MedChemExpress(MCE)產品50000+ 特異性抑制劑、化合物庫、重組蛋白，專注於信號通路和疾病研究領域。全球文獻廣泛引用，質控體系嚴格，品質100%。官網連結 https://www.medchemexpress.com
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