1,3-二苯基異苯並呋喃 (DPBF) | CAS# 5471-63-6 MCE貨號 HY-W011664
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1,3-二苯基異苯並呋喃 (DPBF) 1,3-Diphenylisobenzofuran | CAS# 5471-63-6 MCE貨號 HY-W011664
(Synonyms: DPBF) Purity: 96.82%
1,3-Diphenylisobenzofuran (DPBF) has been developed as a selective probe for the detection and quantitative determination of hydrogen peroxide in samples containing different reactive nitrogen and oxygen species (RNOS). DPBF is a fluorescent probe which, for almost 20 years, was believed to react in a highly specific manner toward some reactive oxygen species such as singlet oxygen and hydroxy, alkyloxy or alkylperoxy radicals.
1,3-二苯基異苯並呋喃 (DPBF) 已被開發為選擇性探針，用於檢測和定量測定含有不同活性氮(reactive nitrogen)和氧物種 ( oxygen species, RNOS) 的樣品中的過氧化氫(hydrogen peroxide)。 DPBF 是一種螢光探針，近 20 年來，人們一直認為它能以高度特異性的方式與某些活性氧物質（例如單線態氧和羥基、烷氧基或烷基過氧自由基）發生反應。
1,3-Diphenylisobenzofuran Chemical Structure
CAS No. : 5471-63-6
Preparation of DPBF working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of DPBF in 3.6992 mL of DMSO to obtain 10 mM of DPBF.
Note: It is recommended to store the stock solution at -20°C, -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of DPBF working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 20 μM of DPBF working solution.
Note: Please adjust the concentration of DPBF working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of DPBF working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Precautions
1. It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of DPBF working solution according to the actual situation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
| Molecular Weight |
270.33 |
|---|---|
| Appearance |
Solid |
| Formula |
C20H14O |
| CAS No. | |
| Emission (Em) |
523 |
| Excitation (Ex) |
495 |
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