自噬泡(autophagic vacuoles)螢光標誌-MDC染劑 | CAS#10121-91-2 MCE貨號HY-D1027
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自噬泡(autophagic vacuoles)螢光標誌 | CAS#10121-91-2MCE貨號HY-D1027
A conventional fluorescent probe, monodansylcadaverine (MDC), has served as a useful fluorescent marker for lysosomal/ autophagic vacuoles. However, it is known to generate high background and weak fluorescent signal.
自噬(Autophagy )
自噬(Autophagy )是一種細胞內降解系統(intracellular degradation system )，可將細胞質成分輸送到溶酶體(lysosome)。自噬作用(Autophagy)廣泛多種生理(physiologica)和病理生理(pathophysiological)作用。不同的選擇性自噬形式( autophagy)導致細胞器或病原體的特異性降解。這些選擇性途徑包括自噬線粒體 (mitophagy)、過氧化物酶體 (pexophagy)、內質網 (reticulophagy 或 ER-phagy) 的降解，核醣體 (ribophagy)、蛋白質聚集體 (aggrephagy)、脂滴 (lipophagy)、胰腺細胞內的分泌顆粒（zymophagy）或細胞內病原體（異體自噬）。自噬由幾個連續的步驟組成: 隔離(sequestration)、運輸到溶酶體( lysosomes)、降解(degradation)和降解產物利用(utilization of degradation products)，每一步都可能發揮不同的作用。自噬信號轉導(Autophagy signal transduction)主要受自噬相關調控基因/蛋白質，Atgs。 ATGs 揭示了自噬體形成的大部分機制。此外，不同的非 ATG蛋白質參與自噬的調節和過程，例如 mTOR、AMPK、AKT、AMBRA1、BCL2、DFCP1 或 VPS34。自噬(Autophagy )及其失調與不同的人類疾病或過程有關，例如癌症(cancer)、神經變性(neurodegeneration)、免疫力(immunity)或衰老(aging)。許多藥物和天然產物都參與自噬調節(autophagy modulation)，無論是通過多種信號通路誘導或抑制細胞自噬。此外，一些治療糖尿病的臨床藥物和化合物也被發現涉及自噬調節。
Dansylcadaverine (Synonyms: Monodansyl cadaverine)
Dansylcadaverine (Monodansyl cadaverine, MDC) 是一種自發螢光化合物(autofluorescent compound )，用於標記自噬泡(autophagic vacuoles)。 Dansylcadaverine (Monodansyl cadaverine, MDC) 是轉谷氨醯胺酶(transglutaminases)的一種高親和力底物，可以阻斷許多配體的受體介導的內吞作用(endocytosis)。Dansylcadaverine (Monodansyl cadaverine, MDC) 的抑制活性反映了其作為轉谷氨酰胺酶( transglutaminases)底物的能力以及競爭性阻斷纖維蛋白分子(fibrin molecules)交聯的能力[2]。dansylcadaverine 是一種陽離子螢光探針，可與細菌脂多醣(bacterial lipopolysaccharide)和脂質 A (lipid A)結合，並被其他對內毒素(endotoxins)具有親和力的化合物競爭性取代[3]。
DMSO : 62.5 mg/mL (186.31 mM; Need ultrasonic)
H2O : 1 mg/mL (2.98 mM; ultrasonic and warming and heat to 80°C)
Description: Dansylcadaverine is an acid dye with green fluorescence, it is a specific marker for autophagic vacuoles, and it can be used for the detection and quantification of autophagy.Method: For Dansylcadaverine staining. (引用文獻)
1. Cells are seeded in 6-well plates at a concentration of 3×104 cells/well in a 5% CO2 incubator at 37°C for 24 h.
2. Following transfection, 50 mM Dansylcadaverine is added to each well for 15 min incubation, and then rinse cells for 3 times with PBS.
3. The fluorescence is visualized under a confocal laser-scanning microscope (TCS 4D; Leica, Heidelberg, Germany).
Description: Dansylcadaverine is an acid dye with green fluorescence, it can be used as a specific autophagolysosome marker to analyze the autophagic process.
Method: For Dansylcadaverine staining. (引用文獻)
1. Cells are cultured on confocal dishes at a density of 1×104 cells/mL overnight and then added with testing compounds for one hour.
2. After stimulation with RANKL (50 ng/mL) for five days, cells are incubated with Dansylcadaverine (50 mM; 15 min; 37°C).
3. Wash cells with 1×PBS for three times with 5 min interval.
4. Finally, the cells are observed under a confocal laser scanning microscopy.
| Molecular Weight |
335.46 |
|---|---|
| Appearance |
Solid |
| Formula |
C17H25N3O2S |
| CAS No. | |
| SMILES |
NCCCCCNS(=O)(C1=CC=CC2=C1C=CC=C2N(C)C)=O |
Dansylcadaverine (Monodansyl cadaverine, MDC) 細胞螢光顯微鏡分析(50 mM)
MCF7 cells (2.4×104 ) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods . The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37°C for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope[2]
5 Publications Citing Use of MCE Dansylcadaverine
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