ATP檢測試劑盒(比色法/螢光法) | ATP Assay Kit (Colorimetric/Fluorometric)APExBIO貨號K2315

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APExBIO系列商品-細胞代謝->>ATP檢測試劑盒(比色法/螢光法)
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ATP檢測試劑盒(比色法/螢光法) | ATP Assay Kit (Colorimetric/Fluorometric)APExBIO貨號K2315
產品介紹
ATP Assay Kit (Colorimetric/Fluorometric)
ATP is the most crucial energy source within cells, playing a vital role in various cellular processes. Abnormal changes in ATP levels are often associated with impaired cellular functions, making the detection of ATP levels highly important.This kit provides a simple and sensitive method for quantifying ATP in tissues, cells, and other samples. Glycerol kinase can phosphorylate glycerol using ATP as the phosphate donor, producing glycerol-3-phosphate. Subsequently, glycerol-3-phosphate is oxidized by glycerol phosphate oxidase, generating hydrogen peroxide (H2O2). In the presence of horseradish peroxidase, the produced H2O2 reacts with the highly sensitive Amplex Red probe to form the fluorescent red product, resorufin. The fluorescence intensity and absorbance of resorufin are directly proportional to the ATP concentration in the sample. Consequently, this kit offers flexible detection, allowing measurements by either colorimetric or fluorometric methods.
ATP 是細胞內最重要的能量來源,在多種細胞生理過程中扮演至關重要的角色。 ATP 水平的異常變化往往與細胞功能受損有關,因此,檢測 ATP 水平具有重要意義。本試劑盒提供了一種簡單且靈敏的方法,用於定量檢測組織、細胞及其他樣本中的 ATP。甘油激酶 (Glycerol kinase)以 ATP 為磷酸基團供體,催化甘油磷酸化生成 3-磷酸甘油 ( glycerol-3-phosphate);隨後3-磷酸甘油 ( glycerol-3-phosphate) 在甘油磷酸氧化酶的作用下被氧化,產生過氧化氫(H2O2)。在辣根過氧化物酶(HRP)存在下,生成的 H2O2 與高靈敏度探針 Amplex Red 反應,生成紅色螢光產物-試鹵靈(resorufin)。試鹵靈的螢光強度及吸光度與樣本中的 ATP 濃度成正比。因此,此試劑盒檢測方式靈活,既可採用比色法,也可採用螢光法進行測定。

| Components | 100 Assays | 500 Assays | Storage |
| ATP Assay Buffer | 25 mL | 125 mL | -20°C |
| Amplex Red | 200 μL | 5 x 200 μL | -20°C away from light |
| Enzyme Solution | 200 μL | 5 x 200 μL | -20°C |
| Cofactor | 200 μL | 5 x 200 μL | -20°C away from light |
| ATP Standard (100 mM) | 10 μL | 50 μL | -20°C |
APExBIO提供優質的胜肽和生物分析試劑,廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域, 還提供定制的服務,包括多肽合成,抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析,高效液相色譜,質譜,和HMNR數據。
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