FITC | CAS# 3326-32-7 MCE貨號HY-66019
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MCE (MedChem Express) -FITC (Synonyms: Fluorescein 5-isothiocyanate)
MCE總部位於美國新澤西，提供種類齊全的化學及生化研究用產品，包括創新的生命科學試劑，reference compounds (標準化合物)，APIs (原料藥) 及Natural compounds (天然化合物)以提供實驗室或科學研究之用，MedChemExpress(MCE)產品50000+ 特異性抑制劑、化合物庫、重組蛋白，專注於信號通路和疾病研究領域。全球文獻廣泛引用，質控體系嚴格，品質100%。
FITC | CAS# 3326-32-7 MCE貨號HY-66019
FITC (Fluorescein Isothiocyanate), is one of the green fluorescein derivatives widely used in biology. FITC has the characteristics of high absorptivity and excellent fluorescence quantum yield. The isothiocyanate group of FITC can be combined with amino, sulfhydryl, imidazole, tyrosyl, carbonyl and other groups on the protein, so as to achieve protein labeling including antibodies and lectins. In addition to its use as a protein marker, FITC can also be used as a fluorescent protein tracer to rapidly identify pathogens by labeling antibodies, or for microsequencing of proteins and peptides (HPLC). The maximum excitation wavelength of FITC is 494 nm. Once excited, it fluoresces yellow-green at a maximum emission wavelength of 520 nm. In addition, FITC is also a hapten that can induce contact hypersensitivity (CHS) and induce an atopic dermatitis model.
FITC（異硫氰酸螢光素）是廣泛應用於生物學的綠色螢光素衍生物(green fluorescein derivatives)之一。 FITC具有高吸收率和優異的螢光量子產率的特性。 FITC的異硫氰酸酯基團可與蛋白質上的氨基、巰基、咪唑基、酪胺酸基、羰基等基團結合，從而實現包括抗體、凝集素在內的蛋白質標記。除了用作蛋白質標記物外，FITC 還可用作螢光蛋白示蹤劑，可透過標記抗體快速識別病原體，或用於蛋白質和胜肽的微定序 (HPLC)。 FITC 的最大激發波長為 494 nm。一旦被激發，它會發出黃綠色螢光，最大發射波長為 520 nm。此外，FITC也是一種半抗原，可以誘發接觸性超敏反應（CHS），誘發異位性皮膚炎模型。
For research use only. We do not sell to patients.
Description: FITC can be used as a fluorescence probe for nanocomposites with green fluorescence.Method: For nanocomposites linking.
1. Add nanocomposite to EDC and NHS (molar ratio: 1:5:5), and the pH is adjusted to activate the carboxyl groups.
2. Dissolve FITC in dimethyl sulfoxideab and add to the above mixture and shaked in the dark overnight.
3. The final FITC-labeled nanocomposite is obtained by lyophilization.
4. Use a probe-based confocal laser endoscopy (Cellvizio, Mauna Kea Technologies, France) for determination.
Description: FITC can be used as a fluorescence probe for nanocomposites with green fluorescence.
Method: For nanocomposites linking.
1. Suspend nanocomposites (20 mg) in PBS (20 mL) with FITC (1 mg) and put the mixture stirred overnight in the dark.
2. Wash nanocomposites for five times with PBS to remove excess FITC.
3. Testing cells are seeded into a 6-well microplate at a density of 105/well and cultured overnight at 37°C in 5% CO2.
4. Change the culture medium with as-prepared medium containing nanocomposite-FITC. After coincubation for 1, 2, 4, and 8 h, testing cells are rinsed three times with PBS.
5. Use a confocal laser scanning microscopy ( FV1000 Olympus, Tokyo, Japan) for image.
Description: FITC can be used as a fluorescence probe for linking to molecule glue with green fluorescence.
FITC Chemical Structure
CAS No. : 3326-32-7
MedChemexpress (MCE) 產品-化學及生化研究用產品
MCE (MedChemexpress) 總部位於美國新澤西，提供種類齊全的化學及生化研究用產品，包括創新的生命科學試劑，reference compounds (標準化合物)，APIs (原料藥) 及Natural compounds (天然化合物)以提供實驗室或科學研究之用，MedChemExpress(MCE)產品50000+ 特異性抑制劑、化合物庫、重組蛋白，專注於信號通路和疾病研究領域。全球文獻廣泛引用，質控體系嚴格，品質100%。官網連結 https://www.medchemexpress.com
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