【亞硝酸鹽檢測】Griess Reagent Abcam (Biovision)貨號k544-200
亞硝酸鹽檢測試劑盒 (Griess Reagent) | 貨號k544-200 亞硝酸鹽（Nitrite; NO2 – ）累積量測定套組-Griess Reagent
在幾乎每個生物體中都發現了Nitrogen-based ions氮基離子，Nitrogen-based ions亞硝酸鹽和nitrate硝酸鹽。此外，在哺乳動物中發現內源亞硝酸鹽水平，也可從dietary sources膳食來源獲得。在人類中，nitrit亞硝酸鹽 (nitrite) 進一步代謝為一氧化氮(Nitric Oxide)和其他活性氮物質（reactive nitrogen species, nitrogen oxides氮氧化物）。硝酸鹽 – 亞硝酸鹽 – NO生化途徑因其參與細胞信號傳導(cell signaling)，缺氧依賴性反應(hypoxia-dependent response )和血流調節而眾所周知。最近的研究表明線粒體中亞硝酸鹽還原為氮氧(Nitrogen Oxygen )。具體而言，肌紅蛋白(myoglobin)和黃嘌呤氧化還原酶(xanthine oxidoreductase)可在低氧條件下產生NO，從而導致線粒體呼吸。BioVision的亞硝酸鹽檢測試劑盒使用Griess Reagent，這是一種用於估算亞硝酸鹽的常用測量方式。在該測定中，使用Griess試劑I將亞硝酸鹽還原為氮氧化物。然後，氮氧化物與Griess Reagent II反應形成穩定的產物，其可通過其在OD 540nm處的吸光度來檢測。一個步驟快速簡單分析，檢測低至1 nmol /孔的亞硝酸鹽水平。
ELISA儀器設備請按此 (標配405、450、492、630 nm濾片/最高可擴充至7濾片）（k544 λ= 540 nm）
| Kit Summary | • Detection method- Absorbance (540 nm) • Species reactivity- Mammalian • Applications- BioVision’s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process. |
| Detection Method | Detection method- Absorbance (540 nm) |
| Species Reactivity | Eukaryotes |
| Applications | Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process. |
| Features & Benefits | • Simple two-step procedure; takes only ~1-1.5 hours • Fast and convenient • BioVision’s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of total nitrite. |
| Kit Components | • Assay Buffer • Griess Reagent R1 • Griess Reagent R2 • Nitrite Standard |
