鏈黴親和素磁珠 (Streptavidin Magnetic Beads) | MCE貨號HY-K0208
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MCE (MedChem Express) -Streptavidin Magnetic Beads
MCE總部位於美國新澤西，提供種類齊全的化學及生化研究用產品，包括創新的生命科學試劑，reference compounds (標準化合物)，APIs (原料藥) 及Natural compounds (天然化合物)以提供實驗室或科學研究之用，MedChemExpress(MCE)產品50000+ 特異性抑制劑、化合物庫、重組蛋白，專注於信號通路和疾病研究領域。全球文獻廣泛引用，質控體系嚴格，品質100%。
鏈黴親和素磁珠 (Streptavidin Magnetic Beads) | MCE貨號HY-K0208
MCE Streptavidin Magnetic Beads provide a fast and convenient method for numerous applications, including purification of proteins and nucleic acids, protein interaction studies, immunoprecipitation, immunoassays, pull-down and cell isolation
MCE 鏈黴親和素磁珠為多種應用提供了一種快速且方便的方法，包括蛋白質和核酸的純化、蛋白質相互作用研究、免疫沉澱(immunoprecipitation)、免疫測定(immunoassays)、 pull-down 和細胞分離 (cell isolation) 鏈黴親和素磁珠(MCE Streptavidin Magnetic Bead)使用重組鏈黴親和素(recombinant streptavidin)共價( covalently)偶聯到磁珠的表面。鏈黴親和素不含碳水化合物基團( carbohydrate group)，與親和素不同，可確保低非特異性結合(low nonspecific binding)。由於鏈黴親和素和生物素之間的高親和力，生物素化分子（例如胜肽、蛋白質、抗體、糖、凝集素、寡核苷酸、DNA/RNA）與珠子結合。使用磁力架手動或使用儀器自動從溶液中去除 MCE 鏈黴親和素磁珠。(請勿離心、乾燥或冷凍磁珠)
1、結合力高。
2.非特異性結合低。
3. 樣品損失最小。
4. 即可使用。
1. Preparation of Magnetic Beads
1.1 Resuspend the Magnetic Beads in the vial (tilt and rotate for 2 minutes or gently pipette for 10 times, do not vortex). Transfer 20 μL of Anti-c-Myc Magnetic Beads suspension into a new tube.
1.2 Add 500 μL of wash buffer to the beads and gently pipette to mix. Place the tube into a magnetic stand (MCE Cat. No.: HY-K0200) to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 2 times
2. Protein Binding
2.1 Add 500 μL of cell lysate (the sample containing c-Myc-tagged protein) to the washed beads. For Ag binding, incubate for 2 hours at room temperature or overnight at 4°C while gently rotating the tube.
2.2 Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant.
NOTE: Occasional aggregation of magnetic beads during the binding process doesn’t affect experimental results.
3. Washing
Add 500 μL of wash buffer to the Magbeads-Ag complex and mix gently. Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 4 times.
4. Elution & Detection
Three elution methods are recommended according to protein characteristics or further usage:
1) Elution with sample buffer for gel electrophoresis and immuoblotting. Add 50 μL of 1× SDS-PAGE loading buffer to each tube and boil for 5 minutes. Cool and place the tube into a magnetic stand to collect the beads and transfer the supernatant to a new tube. Keep the supernatant containing the target antigen for SDS-PAGE analysis
2) Elution with Elution Buffer A under acidic condition. Add 50 μL of Elution Buffer A to each tube. Incubate with gentle shaking or on a rotator for 10 minutes at room temperature. Place the tube into a magnetic stand to collect the beads and transfer the supernatant to a new tube. Adding 25 μL of Neutralization Buffer for each 50 μL of eluate to neutralize the low pH, which may help preserve bioactivity of target protein.
3) Elution with Elution Buffer B under native condition. Add 3-5 (v/v) volume of Elution Buffer B to each tube. Incubate with gentle shaking or on a rotator for 1 hour at room temperature or 2 hours at 4°C. Place the tube into a magnetic stand to collect the beads and transfer the supernatant to a new tube. For immediate use, store the eluates at 4°C, or store at -20°C for long term storage.
MedChemexpress (MCE) 產品
MCE (MedChemexpress) 總部位於美國新澤西，提供種類齊全的化學及生化研究用產品，包括創新的生命科學試劑，reference compounds (標準化合物)，APIs (原料藥) 及Natural compounds (天然化合物)以提供實驗室或科學研究之用，MedChemExpress(MCE)產品50000+ 特異性抑制劑、化合物庫、重組蛋白，專注於信號通路和疾病研究領域。全球文獻廣泛引用，質控體系嚴格，品質100%。官網連結 https://www.medchemexpress.com
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