MCE (MedChem Express) -GW6471 is a potent PPARα antagonist

MCE總部位於美國新澤西，提供種類齊全的化學及生化研究用產品，包括創新的生命科學試劑，reference compounds(標準化合物)，APIs(原料藥)及Natural compounds(天然化合物)以提供實驗室或科學研究之用，MedChemExpress(MCE)產品50000+ 特異性抑制劑、化合物庫、重組蛋白，專注於信號通路和疾病研究領域。全球文獻廣泛引用，質控體系嚴格，品質100%。

GW6471 | CAS# 880635-03-0 MCE貨號HY-15372 

GW6471 is a potent PPARα antagonist. In a cell-based reporter assay, GW6471 completely inhibits GW409544-induced activation of PPARα with an IC50 of 0.24 μM[1]. The functional role of PPARα is evaluated on renal cell carcinoma (RCC) cell viability by MTT assay. Both Caki-1 (VHL wild type) and 786-O (VHL mutated) cells are incubated separately with a specific PPARα agonist, WY14,643, or a specific PPARα antagonist, GW6471 at concentrations from 12.5 to 100 μM for 72 hours, and cell viability is assessed. While WY14,643 either has no affect on, or slightly increased, cell viability, GW6471 significantly and dose-dependently inhibits cell viability (up to approximately 80%) in both cell lines[2].

在基於細胞的報告基因檢測( cell-based reporter assay)中，GW6471 完全抑制 GW409544 誘導的 PPARα 激活，IC50 為 0.24 μM[1]。通過 MTT 測定評估 PPARα 對腎細胞癌 (RCC) 細胞活力的功能作用。將 Caki-1（VHL 野生型）和 786-O（VHL 突變型）細胞分別與濃度為 12.5 至 100 μM 的特定 PPARα 激動劑 WY14,643 或特定 PPARα 拮抗劑 GW6471 孵育 72 小時，並評估細胞活力。雖然 WY14,643 對細胞活力沒有影響或略有增加，但 GW6471 顯著且劑量依賴性地抑制兩種細胞系中的細胞活力（高達約 80%）[2]。

GW6471 Chemical Structure

CAS No. : 880635-03-0

30 Publications Citing Use of MCE GW6471

GW6471 purchased from MCE. Usage Cited in: J Dairy Sci. 2023 Feb 14;S0022-0302(23)00052-8.  [Abstract]

GW6471 significantly reduces the regulatory effects of GINY and DQW on the protein expression levels of PPARα, CPT-1a, ACOX1, HO-1, and FASN in HepG2 cells.

GW6471 purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2019 Nov 3;2019:7536803.  [Abstract]Cells are treated with PE (50 μM), zileuton (100 μM), and GW6471 (10 μM) for 24 h. α-Actinin staining (n = 6) results in H9c2 cells.

GW6471 purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2019 Nov 3;2019:7536803.  [Abstract]The antihypertrophic effects of zileuton ar counteracted by GW6471 as indicated by increased PE-induced hypertrophic marker transcription, cell surface area, and oxidative stress when compared with those in the PE group and PE+ zileuton group.

MedChemexpress (MCE) 產品

MCE (MedChemexpress) 總部位於美國新澤西，提供種類齊全的化學及生化研究用產品，包括創新的生命科學試劑，reference compounds (標準化合物)，APIs (原料藥) 及Natural compounds (天然化合物)以提供實驗室或科學研究之用，MedChemExpress(MCE)產品50000+ 特異性抑制劑、化合物庫、重組蛋白，專注於信號通路和疾病研究領域。全球文獻廣泛引用，質控體系嚴格，品質100%。官網連結 https://www.medchemexpress.com