【碘化丙啶 (Propidium Iodide ) 】 MCE(MedChem Express) 貨號HY-D0815
MCE (MedChem Express) -Propidium Iodide
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碘化丙啶 (Propidium Iodide ) | MCE貨號HY-D0815
碘化丙啶 (Propidium Iodide, PI) 是一種將 DNA 染色的核染色劑。碘化丙啶是溴化乙啶( ethidine bromide )的類似物，嵌入雙股 DNA 後會發出紅色螢光。碘化丙啶 (Propidium Iodide, PI) 不能穿過活細胞膜，但可以穿過受損的細胞膜染色細胞核。碘化丙啶 (Propidium Iodide, PI) 的螢光波長為493/617 nm，與DNA鑲嵌後的波長為536/635 nm。碘化丙啶 (Propidium Iodide, PI) 常用於細胞凋亡（apoptosis）或壞死（necrosis）的檢測，也常用於流式細胞儀分析(flow cytometry analysis)。
Propidium Iodide Chemical Structure
CAS No. : 25535-16-4
Propidium Iodide purchased from MedChemExpress. Usage Cited in: Chinese Pharmacological Bulletin. 2018 May; 34(5): 620-626.
PC12 cells are treated with 1.5 mM Methylglyoxal (MG) for 24 h before pretreatment with Butein (2.5, 5 μM) for 1 h. Propidium iodide (PI) and Hoechst 33342 are used to determine cell apoptosis.
Propidium Iodide purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2018 Nov 28;10(11):3353-3370. [Abstract]
KLE cells are stained by 25 μg/mL PI in fluorescence-activated cell sorting buffer, incubated cells for 30 min in dark environment at room temperature. Cell apoptosis is determined by Annexin V/PI double staining and percentages of cells in each phase are calculated.
Propidium Iodide purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Feb 8. [Abstract]
Propidium iodide (PI) staining for cell death with GCFN and ferroptosis inhibitor ferrostatin-1 treatment for 48 h
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