【細胞衰老檢測/（SOD）活性測定/Catalase過氧化氫酶活性檢測】-Abbkine
細胞衰老檢測試劑盒（β-半乳糖苷酶） | 貨號KTA3030
Cell Senescence Kit (β-Galactosidase Assay)
細胞衰老(Cellular senescence)是正常細胞停止分裂(cell divide)的現象。衰老的細胞(senescent cells)不再能夠複製(replicate)，但它們仍保持代謝活性(metabolically active)，但因阻滯於G1期，失去了對有絲分裂原的反應能力和合成DNA的能力，不能進入S期。並且與衰老相關的β-半乳糖苷酶活性染色(senescence-associated beta-galactosidase activity)呈陽性，這被認為是細胞衰老的生物標記(biomarker)。細胞的衰老(Cellular senescence)可以通過激活癌基因(oncogenes)和細胞-細胞融合(cell-cell fusion)來實現，DNA對活性氧（reactive oxygen species , ROS）升高的反應會導致DNA損傷(DNA damage)，而不是僅僅依賴於細胞分裂的數目。在正常衰老過程(normal aging)中，組織中的衰老細胞數量顯著增加。
細胞是生物結構(biological structure)和功能(function)的基本單位，也是生物衰老(biological aging)的基本單位。細胞衰老(Cellular senescence)在形態上表現為細胞結構(cell structure)的退化，例如核膜凹陷(nuclear membrane depression)，最終導致核膜塌陷(nuclear membrane collapse)，染色質結構(chromatin structure)改變，超二倍體(hyperdiploid)和異常多倍體細胞(polyploid cells)數量增加。細胞膜的脆性(cell membrane fragility)增加。通透性(permeability)降低，膜受體(membrane receptors)的類型和數量以及對配體(ligands)的敏感性發生變化；脂褐素(lipofuscin)在細胞中積累，許多細胞器和細胞內結構發生變性。細胞衰老(Cell senescence)在生理上表現(physiologically manifested)為功能下降和新陳代謝低下，例如細胞週期停滯(cell cycle arrest)，細胞複製能力 (cell replication ability) 喪失，對促有絲分裂刺激(mitogenic stimulation)的反應性減弱以及對促凋亡因子(pro-apoptotic factors)的反應性改變。細胞內酶活性中心被氧化，酶活性(Enzyme activity)降低，蛋白質合成(protein synthesis)降低等。衰老細胞(senescent cells)不再複制，但它們仍保持代謝活性(metabolically active)，並且衰老相關的β-半乳糖苷酶活性(beta-galactosidase activity)呈陽性，這被認為是細胞衰老生物標記(biomarker of cellular senescence)。
產品描述： Abbkine細胞衰老檢測試劑盒（Senescence β-Galactosidase Staining Kit, KTA3030）提供了基於衰老過程中pH值為6的衰老相關β-半乳糖苷酶（SA-β-Gal）活性上調的試劑，用於檢測衰老細胞(senescent cells)或組織(tissues)。細胞或組織的老化可以在普通的光學顯微鏡下觀察。 SA-β-Gal僅存在於衰老細胞(senescent cells)中，在衰老前，靜止，腫瘤或永生細胞中無法檢測到。
Senescence β-Galactosidase Staining Kit provide reagents for detecting the senescent cells or tissues based on the up-regulation of senescence-associated β-galactosidase (SA-β-Gal) activity at pH 6 during aging. The aging of cells or tissues can be observed under a common optical microscope. SA-β-Gal is present only in senescent cells and is undetectable in presenescent, quiescent, tumor or immortal cells
- Kit components
- 10×Fixation Buffer • 10×PBS • Reagent A • Reagent B • Reagent C • X-Gal solution
- Features & Benefits
- Simple and optimized procedure. • Fast and convenient. • Suitable for aging detection of cultured cells and tissue sections. • Cell senescence β-galactosidase staining kit only stains senescent cells, and does not stain pre-senescence cells (senescent cells), quiescent cells (static cells), immortal cells (immortal cells) or tumor cells.
- Storage instructions
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
- Shipping
Gel pack with blue ice.
- Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
超氧化物歧化酶（SOD）活性測定試劑盒 | Superoxide dismutase Activity Assay Kit, 貨號KTB1030
超氧化物歧化酶（Superoxide dismutases , SOD，EC1.15.1.1）是催化超氧化物陰離子(superoxide anion)分解為O2和H2O2的酶。 它們是抵抗暴露於O2的所有細胞中超氧自由基(superoxide radical)毒性的重要抗氧化劑(antioxidant defense against)。 SOD異常活動與肌萎縮性側索硬化(amyotrophic lateral sclerosis)，圍產期致死率(perinatal lethality)，神經疾病(neural disorders)和癌症(cancer)等疾病有關。 超氧化物歧化酶(superoxide dismutase)有三個主要家族：Cu / Zn，Fe / Mn和Ni型。 人類中存在三種形式的超氧化物歧化酶(superoxide dismutase)。 SOD1位於細胞質(cytoplasm)中，SOD2位於線粒體(mitochondria)中，SOD3位於細胞外(extracellular)。
Determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids.
• Determining SOD activity by WST-8 method, the maximum inhibition percentage can be close to 100 %, and it can not be interfered by some common interfering factors.
• A broad range linearity: 3.13- 200U/mL.
Fig. Plot the Standard Curve inhibition rate vs SOD activity.
CheKine™超氧化物歧化酶（SOD）活性測定試劑盒(CheKine™ Superoxide dismutase (SOD) Activity Assay Kit, 貨號KTB1030)為定量測定血清(serum)，血漿(plasma)，組織(tissue)/細胞裂解液(cell lysates)和其他生物液體(biological fluids)中的SOD酶活性(SOD enzyme activity)提供了一種簡便的比色測定法(colorimetric assay)。 在測定中，黃嘌呤氧化酶（xanthine oxidase, XO）催化反應提供了超氧陰離子（superoxide anion , O2-）。 O2-與四唑鎓鹽WST-8染料( tetrazolium salt WST-8 dye)反應生成水溶性 formazan產物。 SOD清除了O2－，因此用於顯色反應的O2－較少。 通過比色法在OD 450nm下測量SOD的這種抑制活性。由於SOD試劑盒（KTB1030）報告為抑制％，因此可以不使用標準試劑。 樣品中的SOD越多，產生的 less WST-8 formazan產物（這取決於黃嘌呤氧化酶（XO）活性）。
- Kit components
- Assay Buffer
• Sample Diluent
• WST-8
• Enhancer
• SOD Standard (200 U/mL)
• Xanthine Oxidase
• Xanthine - Features & Benefits
- Determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids.
• Determining SOD activity by WST-8 method, the maximum inhibition percentage can be close to 100 %, and it can not be interfered by some common interfering factors.
• A broad range linearity: 200-3.13 U/ml. - Usage notes
- Briefly centrifuge small vials at low speed prior to opening.
• Prior to assay, bring all reagents to room temperature (25°C). The Xanthine reagent may appear to be turbid. Briefly vortext this tube before pipetting.
• If not assayed immediately, samples can be stored at -80°C.
Catalase過氧化氫酶活性檢測試劑盒 | CheKine™ Catalase Activity Assay Kit貨號KTB1040
【Catalase過氧化氫酶活性檢測】CheKine™ Catalase Activity Assay Kit
過氧化氫酶（Catalase , CAT）是幾乎在所有活生物體中常見的抗氧化酶(antioxidant enzyme)。它的主要功能是催化過氧化氫(hydrogen peroxide)分解為水(water)和氧氣(oxygen)，並去除體內的過氧化氫(hydrogen peroxide)，從而防止細胞被過氧化氫中毒。它是生物防禦系統(biological defense system)中的關鍵酶之一，為人體提供了抗氧化防禦機制(antioxidant defense mechanism)。
Fig. Formaldehyde standard curve. The y-axis is absorbance of standards and the x-axis is final formaldehyde concentration (uM).
CheKine™過氧化氫酶活性檢測試劑盒(CheKine™ Catalase Activity Assay Kit, KTB1040) 可應用於各種生物樣品（例如Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate）中的過氧化氫酶活性(catalase activity)檢測, 提供了一種簡便的比色測定方法。 該檢測試劑盒基於過氧化氫酶(catalase)與甲醇(methanol)的反應，在最佳濃度的H2O2存在下，利用過氧化氫酶(catalase)的過氧化功能來測量過氧化氫酶活性。 產生的甲醛(formaldehyde)可以在OD 540 nm處進行比色測定。 因此，樣品中存在的過氧化氫酶活性(catalase activity)與獲得的信號成正比。測量線性範圍為 2-75 µM, 最低測量活性為2 U/ml。
商品需用儀器測量 酵素免疫分析儀請按此 (標配405、450、492、630 nm, 可擴增至7個濾片)
Abbkine對於CAT活性測定試劑盒(CAT Activity Assay Kit)，選擇了不同的品牌（Cayman Chemical，Abcam，Biovision），與Abbkine CheKine™過氧化氫酶（CAT）活性測定試劑盒（Cat＃：KTB1040）進行了比較。CAT活性測定試劑盒，Abbkine和Cayman Chemical品牌的測定原理相似。甲醇(Methanol)被用作反應底物。而Abcam和Biovision將H2O2作為反應底物。H2O2不僅是過氧化氫酶（catalase , CAT）的單一底物，樣品中的其他過氧化物酶可能會干擾實驗結果，並且標準曲線誤差可能是由過氧化氫的不穩定性引起的。儘管甲醇(Methanol)是過氧化氫酶的獨特底物，但其他過氧化物酶不能使用甲醇(Methanol)作為底物，因此可以避免訊號干擾。
| Brand | Assay Principle | Sample type |
| Abbkine | 過氧化氫酶活性(catalase activity)檢測, 提供了一種簡便的比色測定方法。 該檢測試劑盒基於過氧化氫酶(catalase)與甲醇(methanol)的反應，在最佳濃度的H2O2存在下，利用過氧化氫酶(catalase)的過氧化功能來測量過氧化氫酶活性。 產生的甲醛(formaldehyde)可以在OD 540 nm處進行比色測定。 因此，樣品中存在的過氧化氫酶活性(catalase activity)與獲得的信號成正比。測量線性範圍為 2-75 µM, 最低測量活性為2 U/ml。 | Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate |
| Cayman Chemical | 該檢測試劑盒基於過氧化氫酶與甲醇的反應，在最佳濃度的H2O2存在下，利用過氧化氫酶的過氧化功能來測量過氧化氫酶活性。 產生的甲醛可以在OD 540 nm處進行比色測定。 因此，樣品中存在的過氧化氫酶活性與獲得的信號成正比。 | Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate |
| Abcam | 樣品中存在的過氧化氫酶與過氧化氫（H2O2）反應生成水和氧氣。 未轉化的H2O2與探針反應生成可以在OD 570 nm處進行比色法測量的產物。 | Serum, Plasma, Cell Lysate, Tissue Lysate, Urine |
| Biovision | 樣品中存在的過氧化氫酶與過氧化氫（H2O2）反應生成水和氧氣。 未轉化的H2O2與探針反應生成可以在OD 570 nm處進行比色法測量的產物。 | Tissue Homogenate, Cell Lysate, erythrocyte lysate |
Kit components
• Assay Buffer
• Sample Diluent
• Formaldehyde standard (4.25 M)
• Catalase (control)
• Potassium Hydroxide
• Hydrogen Peroxide
• Chromogen
• Potassium Periodate
Features & Benefits
• Determination of catalase activity in serum, plasma, tissue/cell lysates and other biological fluids.
• Determining catalase activity directly by utilizing the peroxidatic function of catalase, which can not be interfered by other peroxidases.
• A broad range linearity: 2-75 µM.
• Measure catalase activity down to 2 U/ml
| 貨號 | 產品名稱 | 胞器染色位置 | 螢光波段 | |
| 貨號KTC4003 | TraKine™粒線體染色 | 粒線體(mitochondria) | Ex / Em = 490/523 nm | 增殖(proliferating)和非增殖細胞(non-proliferating cells) |
| 貨號KTC4100 | Pro活細胞微管蛋白染色 | 微管蛋白(Tubulin) | Ex/Em = 500/520 nm | 活細胞或固定之細胞 |
| 貨號KTC4001 | Abbkine 細胞膜染色 | 細胞膜 (plasma membrane) | Ex/Em = 484/501 nm | 活細胞、固定懸浮、貼附細胞 |
| 貨號BMD00063 | DAPI Staining Solution | 細胞核 dsDNA | Ex/Em: 358/461 nm (with DNA) | 染色活細胞和固定細胞 |
| 貨號KTC4210 | 活細胞Lysosome染色 | Lysosome | deep red fluorescent dye , Ex/Em:650/665nm | 染色活細胞和固定細胞 |
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| A22120 | FITC, Goat Anti-Rabbit IgG |
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| ABP57259 | GAPDH Polyclonal Antibody |
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| KTL0210 | LinKine™ FITC Labeling Kit |
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| PRP100274 | Human IL-2 protein |
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