【抗氧化分析試劑】Abbkine促銷優惠活動DM&產品資料

超氧化物歧化酶（SOD）活性測定試劑盒 | Superoxide dismutase Activity Assay Kit, 貨號KTB1030

超氧化物歧化酶（Superoxide dismutases , SOD，EC1.15.1.1）是催化超氧化物陰離子(superoxide anion)分解為O2和H2O2的酶。它們是抵抗暴露於O2的所有細胞中超氧自由基(superoxide radical)毒性的重要抗氧化劑(antioxidant defense against)。 SOD異常活動與肌萎縮性側索硬化(amyotrophic lateral sclerosis)，圍產期致死率(perinatal lethality)，神經疾病(neural disorders)和癌症(cancer)等疾病有關。超氧化物歧化酶(superoxide dismutase)有三個主要家族：Cu / Zn，Fe / Mn和Ni型。人類中存在三種形式的超氧化物歧化酶(superoxide dismutase)。 SOD1位於細胞質(cytoplasm)中，SOD2位於線粒體(mitochondria)中，SOD3位於細胞外(extracellular)。測定血清、血漿、組織/細胞裂解液和其他生物體液中的 SOD 酶活性。 WST-8法測定SOD活性，最大抑制率可接近100%，不受一些常見乾擾因素的干擾。廣泛的線性範圍：3.13-200U/mL。

Determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids.
• Determining SOD activity by WST-8 method, the maximum inhibition percentage can be close to 100 %, and it can not be interfered by some common interfering factors.
• A broad range linearity: 3.13- 200U/mL.

Fig. Plot the Standard Curve inhibition rate vs SOD activity.

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CheKine™超氧化物歧化酶（SOD）活性測定試劑盒(CheKine™ Superoxide dismutase (SOD) Activity Assay Kit, 貨號KTB1030)為定量測定血清(serum)，血漿(plasma)，組織(tissue)/細胞裂解液(cell lysates)和其他生物液體(biological fluids)中的SOD酶活性(SOD enzyme activity)提供了一種簡便的比色測定法(colorimetric assay)。在測定中，黃嘌呤氧化酶（xanthine oxidase, XO）催化反應提供了超氧陰離子（superoxide anion , O2-）。 O2-與四唑鎓鹽WST-8染料( tetrazolium salt WST-8 dye)反應生成水溶性 formazan產物。 SOD清除了O2－，因此用於顯色反應的O2－較少。通過比色法在OD 450nm下測量SOD的這種抑制活性。由於SOD試劑盒（KTB1030）報告為抑制％，因此可以不使用標準試劑。樣品中的SOD越多，產生的 less WST-8 formazan產物（這取決於黃嘌呤氧化酶（XO）活性）。

Abbkine CheKine™ Superoxide dismutase (SOD) Activity Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of SOD enzyme activity in serum, plasma, tissue/cell lysates and other biological fluids. In the assay, superoxide anion (O2－) is provided by xanthine oxidase (XO) catalyzed reaction. O2－ reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product. SOD scavenges the O2－ thus less O2－ is available for the chromogenic reaction. This inhibition activity of SOD is measured by colorimetric method at OD 450 nm.

Catalase過氧化氫酶活性檢測試劑盒 | CheKine™ Catalase Activity Assay Kit貨號KTB1040

【Catalase過氧化氫酶活性檢測】CheKine™ Catalase Activity Assay Kit

過氧化氫酶（Catalase , CAT）是幾乎在所有活生物體中常見的抗氧化酶(antioxidant enzyme)。它的主要功能是催化過氧化氫(hydrogen peroxide)分解為水(water)和氧氣(oxygen)，並去除體內的過氧化氫(hydrogen peroxide)，從而防止細胞被過氧化氫中毒。它是生物防禦系統(biological defense system)中的關鍵酶之一，為人體提供了抗氧化防禦機制(antioxidant defense mechanism)。

Fig. Formaldehyde standard curve. The y-axis is absorbance of standards and the x-axis is final formaldehyde concentration (uM).

CheKine™過氧化氫酶活性檢測試劑盒(CheKine™ Catalase Activity Assay Kit, KTB1040) 可應用於各種生物樣品（例如Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate）中的過氧化氫酶活性(catalase activity)檢測, 提供了一種簡便的比色測定方法。該檢測試劑盒基於過氧化氫酶(catalase)與甲醇(methanol)的反應，在最佳濃度的H2O2存在下，利用過氧化氫酶(catalase)的過氧化功能來測量過氧化氫酶活性。產生的甲醛(formaldehyde)可以在OD 540 nm處進行比色測定。因此，樣品中存在的過氧化氫酶活性(catalase activity)與獲得的信號成正比。測量線性範圍為 2-75 µM, 最低測量活性為2 U/ml。

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商品需用儀器測量酵素免疫分析儀請按此 (標配405、450、492、630 nm, 可擴增至7個濾片)

Abbkine對於CAT活性測定試劑盒(CAT Activity Assay Kit)，選擇了不同的品牌（Cayman Chemical，Abcam，Biovision），與Abbkine CheKine™過氧化氫酶（CAT）活性測定試劑盒（Cat＃：KTB1040）進行了比較。CAT活性測定試劑盒，Abbkine和Cayman Chemical品牌的測定原理相似。甲醇(Methanol)被用作反應底物。而Abcam和Biovision將H2O2作為反應底物。H2O2不僅是過氧化氫酶（catalase , CAT）的單一底物，樣品中的其他過氧化物酶可能會干擾實驗結果，並且標準曲線誤差可能是由過氧化氫的不穩定性引起的。儘管甲醇(Methanol)是過氧化氫酶的獨特底物，但其他過氧化物酶不能使用甲醇(Methanol)作為底物，因此可以避免訊號干擾。

CheKine™總抗氧化劑能力分析 | CheKine™ Total Antioxidant Capacity (TAC) Assay Kit貨號KTB1500 偵測範圍: 0.15-3 mM

Fig. Standard Curve of Total Antioxidant Capacity (TAC) Assay. The y-axis is OD593 and the x-axis is Fe2+ concentration (mM).

產品描述：CheKine™總抗氧化劑能力（Total Antioxidant Capacity , TAC）測定試劑盒提供了一種簡單易用的比色測定法，用於測量血清，血漿，細胞培養上清液，尿液，組織/細胞裂解液和其他生物流體中的總抗氧化劑能力。在該測定中，Fe3 + -TPTZ被抗氧化劑還原為Fe2 + -TPTZ。酶催化的反應產物Fe2 + -TPTZ可以在比色讀數為593 nm的條件下進行測量。偵測範圍: 0.15-3 mM。可應用於以下樣品來源: serum, plasma, cell culture supernatants, urine, tissue/ cell lysates and other biological fluids.

硫氧還蛋白過氧化物酶（TPX）檢測 | CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit貨號 KTB1660

穀胱甘肽 (Glutathione) 研究的意義

穀胱甘肽是含有谷氨酸 (glutamate)，半胱氨酸(cysteine)和甘氨酸(glycine)的三肽，其中含有巰基(sulfhydryl groups)。它廣泛存在於動植物中。它具有抗氧化劑(antioxidant)和綜合解毒(detoxification)作用。其相關的輔酶(coenzyme)是NADPH。人體新陳代謝產生的自由基(free radicals)過多會破壞生物膜(biofilm)，侵入生命大分子，加速人體衰老(aging)，並誘發腫瘤(tumors)或動脈粥樣硬化(atherosclerosis) 的產生。穀胱甘肽具有增強生物體的抗氧化功能，促進生長以及增強生物體對人體不利環境的影響的功能。它的主要生理作用是清除體內的自由基。它也是一種很好的解毒劑，可以減弱有毒物質的毒性並從體內清除有毒物質。穀胱甘肽還促進生長並提高人體免疫力。穀胱甘肽(Glutathione)不僅可以用作藥物，還可以用作功能性食品的基礎材料，並且廣泛用於功能性食品中，例如抗衰老(anti-aging)，增強免疫力(enhancing immunity)和抗腫瘤作用(anti-tumor)。

硫氧還蛋白過氧化物酶（TPX）屬於過氧化物酶家族 (peroxidases)。它主要通過減少體內的過氧化氫 (reducing hydrogen peroxide)和某些氫過氧化物(hydroperoxides)來實現抗氧化(anti-oxidation)作用。它的功能類似於GPX，並且是穀胱甘肽氧化還原循環 (glutathione redox cycle) 中的關鍵酶之一。硫氧還蛋白過氧化物酶（TPX）在各種生物中無處不在，例如酵母(yeast)，植物(plants)，動物(animals)，原生動物(protozoa)，寄生蟲(parasites)，細菌(bacteria)和古細菌(archaea)，並且在進化上非常保守(conservative)。硫氧還蛋白過氧化物酶（TPX）與細胞增殖(cell proliferation)，分化(differentiation)，凋亡(apoptosis)和腫瘤發生(tumorigenesis)的調節密切相關。硫氧還蛋白過氧化物酶（TPX）的主要功能包括細胞排毒(cell detoxification)，抗氧化(anti-oxidation)以及調節過氧化氫(hydrogen peroxide)介導的信號轉導(signal transduction)和免疫反應。

Kit components• Assay Buffer
• Substrate
• H2O2
Features & Benefits• Determination of TPX activity in serum, plasma, tissue/cell lysates and other biological fluids.
Usage notes• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• The whole process needs to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution.
• In the detection of TPX activity in cells, the cell number must be between 3-5 x 106, and the extraction of TPX in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate.
Storage instructionsStorage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
ShippingGel pack with blue ice.

Kit components	• Assay Buffer (10X) • Substrate Diluent • Substrate • Reaction Buffer • ascorbic acid (positive control)
Features & Benefits	• Determination of Total Antioxidant Capacity in serum, plasma, cell/tissue samples, urine and bacteria samples. • Using a stronger antioxidant, sascorbic acid as positive control, instead of the frequently-used Trolox. • The reaction is carried out under acidic conditions, which can inhibit some endogenous interference factors.
Usage notes	• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range. • Fresh samples are necessary for good results. If not assayed immediately, samples can be stored at -80°C for one month. • Can not use EDTA as the anticoagulant for serum and plasma samples. The samples also can not contain DTT, Mercaptoethanol, Tween, Triton, NP-40.
Storage instructions	Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

CheKine™總抗氧化劑能力分析 | CheKine™ Total Antioxidant Capacity (TAC) Assay Kit貨號KTB1500 偵測範圍: 0.15-3 mM

Fig. Standard Curve of Total Antioxidant Capacity (TAC) Assay. The y-axis is OD593 and the x-axis is Fe2+ concentration (mM).

硫氧還蛋白過氧化物酶（TPX）檢測 | CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit貨號 KTB1660

穀胱甘肽 (Glutathione) 研究的意義

Kit components• Assay Buffer
• Substrate
• H2O2
Features & Benefits• Determination of TPX activity in serum, plasma, tissue/cell lysates and other biological fluids.
Usage notes• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• The whole process needs to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution.
• In the detection of TPX activity in cells, the cell number must be between 3-5 x 106, and the extraction of TPX in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate.
Storage instructionsStorage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
ShippingGel pack with blue ice.

CheKine™ 花青素還原酶（ANR）活性比色測定試劑盒 | CheKine™ Anthocyanidin Reductase(ANR) Activity Colorimetric Assay Kit貨號KTB3010

產品描述：

花青素還原酶（Anthocyanidin reductase , ANR）是原花青素(procyanidins)生物合成的關鍵酶，催化花青素(anthocyanidins)生成cis-flavane-3-醇，在植物黃酮類化合物(flavonoids)的合成和花青素(anthocyanins)的積累中起重要作用。

CheKine™ 花青素還原酶 (CheKine™ Anthocyanidin reductase (ANR) Activity Colorimetric Assay Kit , 貨號KTB3010) 活性比色測定試劑盒，為檢測 ANR 花青素還原酶活性提供了方便的工具。其原理是 ANR 催化從乙醯花青素和 NADPH 產生黃烷-3-醇和 NADP+。 NADPH 在 340 nm 處有一個吸收峰，但 NADP+ 沒有。 ANR 的酶活性通過檢測 340 nm 處的吸收減少率來計算。

Product name	CheKine™ Anthocyanidin Reductase(ANR) Activity Colorimetric Assay Kit
Applications notes	CheKine™ Anthocyanidin reductase (ANR) Activity Colorimetric Assay Kit provides a convenient tool for detection of ANR Activity. The principle is that ANR catalyzes the production of flavane-3-alcohol and NADP+ from acetyl anthocyanins and NADPH. NADPH has an absorption peak at 340 nm, but NADP+ does not. The enzyme activity of ANR was calculated by detecting the rate of decrease in absorption at 340 nm
Kit components	•Extraction Buffer •Reagent I •Reagent II •Reagent III •Reagent IV
Features & Benefits	• Determination of Anthocyanidin reductase (ANR) in plant tissues. • Detailed sample preparation and result calculation methods are provided.
Storage instructions	Storage at-20°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Background	Anthocyanidin reductase (ANR) is a key enzyme in the biosynthesis of procyanidins, catalyzing anthocyanidins to producecis-flavane-3-alcohols and plays an important role in the synthesis of flavonoids and accumulation of anthocyanins in plants.

黃酮類化合物檢測套組CheKine™ Plant Flavonoids Colorimetric Assay Kit | 貨號KTB1530

黃酮類化合物(Flavonoids)是一類多苯基化合物(polyphenyl compounds)，是植物的次生代謝產物(secondary metabolites of plants)。它們對人體具有抗炎(anti-inflammatory)、抗菌(antibacterial)、降血脂(hypolipidemic effects)、清除體內羥基自由基(scavenging hydroxyl free radicals)、預防癌症(preventing cancer)等作用。槲皮素(Quercetin)是一種典型的類黃酮(flavonoid)。

Fig. Standard Curve of flavonoid assay. The x-axis is OD502 and the y-axis is quercetin concentration (mg/mL) .

產品描述：

該試劑盒提供了一種檢測植物組織(plant tissues)中類黃酮(flavonoid)含量的簡單方法。在鹼性亞硝酸鹽溶液()alkaline nitrite solution中，植物樣品中的黃酮類化合物(flavonoids)和鋁離子(aluminum ions)形成紅色絡合物(red complex)，在502 nm處有特徵吸收峰。通過測量樣品提取物在 502 nm 處的吸光度，可以計算出樣品中黃酮類化合物(flavonoid)的含量。

Product name	CheKine™ Plant Flavonoids Colorimetric Assay Kit
Applications notes	The kit provides a simple method for detecting the flavonoid content in plant tissues. In the alkaline nitrite solution, the flavonoids and aluminum ions in the plant samples form a red complex with a characteristic absorption peak at 502 nm. The flavonoid content of the sample can be calculated by measuring the absorbance of the sample extract at 502 nm.
Alternative	bioflavonoids
Kit components	• Nitrite Solution • Chromogen • NaOH Solution • Quercetin Standard（10mg/mL）
Features & Benefits	• Determination of flavonoid content in plant tissues. • A broad range linearity: 0.156-10 mg/g. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. • Standard and standard curve line are provided.
Calibration range	0.156-10 mg/g
Limit of detection	0.078 mg/g
Usage notes	• Do not mix or substitute reagents or materials from other kit lots or vendors. • Avoid foaming or bubbles when mixing or reconstituting components. • Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay. • Each sample needs to set up a control well, the OD value is greater than 0.6, the sample is appropriately diluted and then measured, pay attention to the calculation formula multiplied by the dilution factor. • Measure immediately after color development, and the absorbance will decrease after 2 hours.
Storage instructions	Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

多酚氧化酶檢測套組CheKine™ Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit | 貨號KTB1140

多酚氧化酶（Polyphenol Oxidase , PPO）廣泛存在於動物、植物、微生物(microorganisms)和培養細胞(cultured cells)中。作為一種含銅氧化酶(copper-containing oxidase)，PPO可以通過氧化一元酚 (oxidizing monohydric phenols)和二元酚 (dihydric phenols) 產生醌 (quinones)而引起褐變(browning)。這種酶還與果蔬加工(fruit and vegetable processing)、茶葉品質(tea quality)和組織培養(tissue culture)密切相關。

產品描述：

PPO多酚氧化酶試劑盒為檢測血清(serum)、血漿(plasma)、組織(tissues)、細胞(cells)、植物(plants)和細菌(bacteria)等多種生物樣品中的 PPO 活性提供了一種簡單的方法。在該測定中，多酚氧化酶（PPO）催化兒茶酚生成醌，其在525 nm處具有特徵吸收峰(absorption peak)。在 525 nm 處醌的增加率可以反映 PPO 的活性。

Product name	CheKine™ Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit
Applications notes	The kit provides a simple method for detecting PPO activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. In the assay, Polyphenol Oxidase (PPO) catalyzes catechol to generate quinone which has a characteristic absorption peak at 525 nm. The rate of quinone increase at 525 nm can reflect PPO activity.
Alternative	PPO; Polyphenol Oxidase
Kit components	• Extraction Buffer • Reagent I • Reagent II • Reagent III
Features & Benefits	• Determination of PPO activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.
Usage notes	• Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay. • The optimal reaction temperature of polyphenol oxidase for different samples is slightly different, and it can be adjusted between 25-37℃.
Storage instructions	Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping	Gel pack with blue ice.

產品貨號	產品品項	反應	測量儀器
貨號KTB1030	超氧化物歧化酶（SOD）活性測定試劑盒	48個反應 96個反應	200-3.13 U/ml
貨號KTB1040	Catalase過氧化氫酶活性檢測試劑盒	96個反應	OD 540 nm 2-75 µM
貨號KTB1500	總抗氧化劑能力TCA分析	96個反應/ 480個反應	593 nm 偵測範圍: 0.15-3 mM
貨號 KTB1660	硫氧還蛋白過氧化物酶（TPX）檢測	48個反應/ 96個反應	240nm
貨號KTB1600	穀胱甘肽(Glutathione)檢測	48個反應 96個反應	412 nm 2-200 µg/mL
貨號KTB3010	CheKine™ 花青素還原酶（ANR）活性比色測定試劑盒	96個反應	absorption at 340 nm
貨號KTB1530	黃酮類化合物檢測套組	48個反應 96個反應	absorption peak at 502 nm 0.156-10 mg/g
貨號KTB1140	多酚氧化酶檢測套組	48個反應 96個反應	absorption peak at 525 nm