H2O2過氧化氫檢測 | CheKine™ Hydrogen Peroxide (H2O2) Assay Kit 貨號KTB1041
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CheKine™ Hydrogen Peroxide (H2O2) Assay Kit | 貨號KTB1041 96個分析 480個分析
Detect biological samples at a level of 1-20 µM.
過氧化氫 (Hydrogen Peroxide , H2O2)，一種活性氧代謝(reactive oxygen metabolic) 副產物，既是細胞內信號(intracellular signaling messenger)的信使，也是氧化應激(oxidative stress)的來源。過氧化氫(Hydrogen peroxide)通過多種機制在細胞中產生，例如中性粒細胞(neutrophils)和巨噬細胞(macrophages)產生 NOX 介導的 ROS（NOX-mediated ROS production），或由線粒體呼吸(mitochondrial respiration)過程中electron leak產生的超氧陰離子歧化酶(dismutase of superoxide anions)產生。通過 NF-κB 和其他因素起作用，氫過氧化物介導的通路與哮喘(asthma)、炎性關節炎(inflammatory arthritis)、動脈粥樣硬化(atherosclerosis)、糖尿病血管病變(diabetic vasculopathy)、骨質疏鬆症(osteoporosis)、神經退行性疾病(neurodegenerative diseases)、唐氏綜合症(Down’s syndrome)和免疫系統疾病(immune system diseases)有關。
CheKine™ 過氧化氫檢測試劑盒(CheKine™ Hydrogen Peroxide Assay Kit, KTB1041) 提供了一種簡單易用的比色檢測方法，用於測量血清(serum)、血漿(plasma)、細胞培養上清液(cell culture supernatants)、組織(tissue)/細胞裂解液(cell lysates)和其他生物液體(other biological fluids)中的過氧化氫(hydrogen peroxide)。在該測定中，H2O2 將亞鐵氧化(H2O2 oxidize ferrous)為三價鐵離子(ferric ion)，二甲苯酚橙(xylenol orange)以高選擇性結合三價鐵離子(ferric ion)，形成有色（紫色）產物，可通過比色法在 OD 580 nm 處進行測量。樣品中存在的過氧化氫與獲得的信號成正比。
ELISA儀器設備請按此 (標配405、450、492、630 nm濾片/最高可擴充至7濾片）（KTB1041 λ= 580 nm）
| Product name | CheKine™ Hydrogen Peroxide (H2O2) Assay Kit |
| Applications notes | CheKine™ Hydrogen Peroxide Assay Kit provides a simple and easy colorimetric assay for measuring hydrogen peroxide in serum, plasma, cell culture supernatants, tissue/cell lysates and other biological fluids. In the assay, H2O2 oxidize ferrous to ferric ion, and xylenol orange binds ferric ion with high selectivity to form a colored (purple) product that can be measured by colorimetric method at OD 580 nm. The Hydrogen Peroxide present in the sample is proportional to the signal obtained. |
| Kit components | • Reaction Buffer • H2O2 Standard (1M) • Assay Buffer (10X) |
| Features & Benefits | • Determination of Hydrogen Peroxide in serum, plasma, cell/tissue samples, urine and other biological fluids. • Detect biological samples at a level of 1-20 µM. • Simple procedures, fase and convenient, and more stable. |
| Usage notes | • It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range. • Fresh samples are necessary for good results. If not assayed immediately, samples can be stored at -80°C for one month. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
| Product name | PurKine™ His-Tag Cu-IDA Resin |
| Applications notes | PurKine™ His-Tag Cu-IDA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 4% agarose, to which Iminodiacetic Acid (IDA) has been coupled. The chelating group has then been charged with copper ions (Cu2+). Tests confirm that performance equals or exceeds popular Cu-IDA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. |
| Alternative | Ni-NTA; His resin; Ni-NTA resin; His tag resin |
Product Properties
| Formulation | Liquid solution |
| Features & Benefits | • High capacity—More than 50 mg of 6xHis-tagged protein per milliliter of resin. • Versatile—For purification under native or denaturing conditions. • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits. |
| Storage buffer | Liquid in PBS buffer containing 20% ethanol. |
| Storage instructions | Stable for one year at 2-8°C from date of shipment. Do not freeze. |
| Shipping | Blue ice |
| 貨號 | 產品名稱 | 胞器染色位置 | 螢光波段 | |
| 貨號KTC4003 | TraKine™粒線體染色 | 粒線體(mitochondria) | Ex / Em = 490/523 nm | 增殖(proliferating)和非增殖細胞(non-proliferating cells) |
| 貨號KTC4100 | Pro活細胞微管蛋白染色 | 微管蛋白(Tubulin) | Ex/Em = 500/520 nm | 活細胞或固定之細胞 |
| 貨號KTC4001 | Abbkine 細胞膜染色 | 細胞膜 (plasma membrane) | Ex/Em = 484/501 nm | 活細胞、固定懸浮、貼附細胞 |
| 貨號BMD00063 | DAPI Staining Solution | 細胞核 dsDNA | Ex/Em: 358/461 nm (with DNA) | 染色活細胞和固定細胞 |
| 貨號KTC4210 | 活細胞Lysosome染色 | Lysosome | deep red fluorescent dye , Ex/Em:650/665nm | 染色活細胞和固定細胞 |