【DNA Damege損傷/細胞Stress 】Abbkine促銷優惠活動DM&產品資料
【細胞增殖&毒理&衰老】| DNA Damege損傷/細胞Stress | 細胞凋亡&細胞染色 | 發炎反應ELISA分析 | 膽固醇代謝/代謝試驗 | 生化檢測試劑盒 | 抗體蛋白純化&其它 | Tag 標籤單多株抗體

CheKine™脂質過氧化檢測試劑盒| CheKine™ Lipid Peroxidation (MDA) Assay Kit, MDA貨號KTB1050
氧化應激(Oxidative stress)與包括心血管疾病(chronic diseases)在內的許多慢性疾病的病因有關。 脂質過氧化(Lipid peroxidation)是由於氧化損傷(oxidative damage)而發生的脂質(lipids)降解(degradation),是氧化應激(oxidative stress)的有用標記(useful marker)。 多不飽和脂質(Polyunsaturated lipids)通常易受活性氧(reactive oxygen)的影響而受到氧化攻擊,從而導致產生明確的鏈反應,並生成最終產物,如丙二醛(malondialdehyde ,MDA)脂質過氧化(Lipid peroxidation)可能導致許多疾病的病理,包括動脈粥樣硬化(atherosclerosis),糖尿病(diabetes)和阿爾茨海默氏病(Alzheimer’s)。

CheKine™脂質過氧化(CheKine™ Lipid Peroxidation (MDA) Assay Kit, MDA)檢測試劑盒為檢測各種樣品中存在的丙二醛(malondialdehyde, MDA)提供了便利的工具。 MDA與4-羥基壬烯醛(4-hydroxynonenal , 4-HNE)一起是脂質過氧化(lipid peroxidation)作用的天然副產物,其定量通常用作脂質過氧化(lipid peroxidation)作用的標記。 樣品中的MDA與硫代巴比妥酸(thiobarbituric acid ,TBA)反應生成MDA-TBA加合物,可以很容易地在OD 532 nm處進行比色定量。 同時,測量600nm處的吸光度,並通過532nm和600nm處的吸光度之差計算MDA(以避免蔗糖(saccharose) 的干擾)。1-100 µM/L
CheKine™ Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct,which can be easily quantified colorimetrically at OD 532 nm. Meanwhile, the absorbance at 600 nm was measured , and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm (To avoid the interference of saccharose).
Fig. Typical MDA standard calibration curve. The y-axis is ΔOD and the x-axis is MDA concentration (uM).
| Product name | CheKine™ Micro Lipid Peroxidation (MDA) Assay kit |
| Kit components | • Extraction Buffer • Reaction Mix |
| Features & Benefits | • Determination of MDA in serum, plasma, urine, animal and plant tissues, cells, bacteria and other biological fluids. • Fast and convenient, measuring lipid peroxidation in a wide range of samples. |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C. |
| Storage instructions | Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Background | Oxygen free radicals react on lipid unsaturated fatty acids to produce lipid peroxidation. The latter gradually breaks down into a series of complex compounds, including malondialdehyde (MDA). Lipid oxidation levels can be measured by detecting MDA levels. Lipid peroxidation may contribute to many diseases, including atherosclerosis, diabetes, and Alzheimer’s disease. CheKine™ Micro Lipid Peroxidation (MDA) Assay kit provides a convenient tool for detection of the malondialdehyde (MDA) |
CheKine™活性氧(ROS)檢測分析試劑盒(螢光法)CheKine™ Reactive Oxygen Species (ROS) Detection Assay Kit (Fluorometric) | 貨號KTB1910
CheKine™ Reactive Oxygen Species (ROS) Detection Assay Kit (Fluorometric) 10 mM 50個反應/100個反應 (以6well, 1ml體積為一個反應)
CheKine™活性氧(CheKine™ Reactive Oxygen Species , ROS)檢測分析試劑盒提供了一種簡單,靈敏,快速的ROS檢測方法。 ROS檢測基於螢光探針DCFH-DA。 DCFH-DA是一種可滲透細胞的靈敏探針,用於檢測細胞內活性氧(reactive oxygen species , ROS)。 該DCFH-DA探針可在穿過活細胞膜時被細胞中的酯酶水解為DCFH。 DCFH沒有螢光並且不能透過細胞膜,然後可以被ROS氧化並產生螢光DCF。 然後可以通過流式細胞儀或螢光顯微鏡檢測螢光,並據此分析細胞中活性氧(CheKine™ Reactive Oxygen Species , ROS)的含量。

活性氧物種(Reactive Oxygen Species, Ros ) Ros是氧正常代謝的天然副產物,在細胞信號傳導(cell signaling)和體內平衡(vivo balance)中起重要作用。 但是,在氧化應激相關狀態(oxidative stress correlation state)下,ROS水平可以大大提高。 ROS的積累將嚴重破壞細胞結構(cell structure)。 氧化應激(Oxidative stress)在心血管疾病(cardiovascular disease),糖尿病(diabetes),骨質疏鬆(osteoporosis),中風(stroke),炎性疾病(inflammatory disease)以及神經退行性疾病(neurodegenerative diseases)和癌症(cancer)等研究中起著重要作用。 ROS檢測將有助於確定氧化應激(oxidative stress)如何調節各種細胞內途徑(intracellular pathways)。Reactive Oxygen Species, Ros is natural by-products of oxygen normal metabolism, play an important role in cell signaling and in vivo balance. However, in the oxidative stress correlation state, the ROS level can be greatly increased. The accumulation of ROS will seriously damage the cell structure. Oxidative stress plays an important role in the research of cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory disease, and neurodegenerative diseases and cancer,etc. ROS detection will help determine how oxidative stress adjusts various intracellular pathways.
| Kit components | • DCFH-DA (10 mM) |
| Features & Benefits | • provides a simple, sensitive, rapid ROS detection method. • based on the fluorescent probe DCFH-DA . • suitable for flow cytometry or fluorescence microscopy. • Provides detailed sample preparation and results calculation methods. |
| Usage notes | • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • The working concentration of positive control H2O2 should be 100 μM (Recommend during 50-200 μM depend on the cell types ). The level of Reactive Oxygen Species (ROS) will increase significantly after stimulated 30 min-4 h. The level of Reactive Oxygen Species (ROS) maybe very different for different cells while stimulating with the positive control. The stimulating time and the concentration of positive control could be prolonged or increased, if there is no obvious rise after 30 min . Or shorten the time or reduce the concentration of positive control, while the fluorescent signal is too strong. • During the experiment, the fluorescent probe DCFH-DA can be diluted in 1: 2000-1: 5000, and make the final concentration of DCFH-DA to 2-5 μM if the signal of negative control also too strong. The time of probe incubating can be adjusted in 15-60 minutes, and minimize the exposure time while reading the film, and the exposure time of the control group and test groups should to be consistent. • Reactive Oxygen Species (ROS) positive control (H2O2) is only used as a sample as a positive control, and not all test groups need positive control. • Please wash away the unbound prode after incubating the prode , otherwise it will cause a high background. • Try to shorten the detection time after incubating the probe (except for stimulation time) to reduce various possible errors. |
| Storage instructions | Please store the whole kit at -20°C upon receipt ,and protect from light. The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
引用文獻
LncRNA SNHG4 regulates miR-10a/PTEN to inhibit the proliferation of acute myeloid leukemia cells(KTP3006-1.472) 作者:Yuan Z, Wang W. 雜誌名稱:Hematology IF:1.472
Abbkine一氧化氮(NO)檢測試劑盒 | 貨號KTB1400 1-100 µM

一氧化氮(NO)是一種反應性自由基,在神經傳遞、免疫反應、細胞凋亡等重要生理功能中發揮重要作用。一氧化氮(NO)是由L-精氨酸經NO合酶(NOS)合成的,已被鑒定為內皮源性舒張因數(EDRF)和抗血小板物質。它是由中性粒細胞和活化巨噬細胞產生的神經遞質和細胞毒性物質。雖然一氧化氮(NO)在生物系統中的分子作用是多種多樣的,但一氧化氮(NO)最重要的作用是啟動鳥苷酸環化酶。
Abbkine 一氧化氮(NO)(Nitric Oxide Assay Kit , NO)檢測試劑盒,應用原理為一氧化氮(NO) 被氧化成亞硝酸鹽和硝酸鹽,測定亞硝酸鹽和硝酸鹽的總量來算一氧化氮(NO)總量。 採用改良Griess 方法將硝酸鹽還原為亞硝酸鹽後,準確測定 一氧化氮(NO)的量。Griess分析機理為重氮類之間的偶氮耦合,重氮類是由磺胺(sulfanilamide)和 NO2-和 N-(1-萘基)乙二月安二yan酸鹽產生的,產生與 一氧化氮(NO)代謝物成正比的比色(540 nm)產物。該反應原理的優勢:Abbkine的一氧化氮(NO)檢測試劑盒,首先將硝酸鹽還原為亞硝酸鹽,結果更為準確。一一氧化氮(NO)溶於液體後會生成亞硝酸鹽和硝酸鹽,亞硝酸鹽和硝酸鹽的相對含量變化很大,最準確確定總一氧化氮(NO)量,需同時測量硝酸鹽和亞硝酸鹽。檢測範圍:1-100 µM
Fig. Standard Curve of Nitric Oxide Assay. The y-axis is OD540 and the x-axis is NO2- concentration (μM).
選擇Abbkine一氧化氮(NO)檢測試劑盒的理由:
1. 性價比高,節約實驗成本;
2. 部分樣本不需要預處理,尿液和唾液不需要脫蛋白;
3. 反應條件溫和,需要的儀器成本低;
4. 有多個規格,客戶可以按需選擇;
5. 快速可靠,使用優化的VCl3試劑,將NO3 -還原為NO2 -所需時間更短;
6. 該方法簡單、高通量,將樣品與試劑混合,孵育並讀取光密度,可以很容易地自動測量成千上萬的樣品。
CheKine™ Superoxide anion Scavenging Capacity Assay Kit
產品描述:超氧陰離子(superoxide anion , O2-)是通過將電子加到氧中而產生的一種短暫的自由基(short-lived radical production)。它是根據環境因素(例如紫外線,香煙煙霧,環境污染物和g射線輻射)形成的,或衍生自黃嘌呤氧化酶或NADPH氧化酶等氧化酶。 O2-一旦形成,就會攻擊細胞成分並破壞脂質,蛋白質和DNA。這會引發多種疾病,包括癌症(diseases),動脈粥樣硬化(atherosclerosis),類風濕性關節炎(rheumatoid arthritis),糖尿病(diabetes),肝損害(liver damage)和中樞神經系統疾病(central nervous system disorders)。CheKine™超氧陰離子清除測定試劑盒(CheKine™ Superoxide anion Scavenging Capacity Assay Kit) 為研究組織/細胞裂解液和其他生物液中的超氧陰離子清除能力,提供了一種簡便的比色測定法。在該測定中,黃嘌呤氧化酶(xanthine oxidase , XO)催化反應提供了超氧陰離子(superoxide anion , O2-)。 O2-與四唑鎓鹽WST-8染料反應形成水溶性的有色formazan產物,可以很容易地進行比色定量。樣品清除了O2-,因此用於顯色反應的O2-較少。通過比色法在OD 450nm下測量樣品的這種清除能力。
| Product name | CheKine™ Micro Superoxide anion Scavenging Capacity Assay Kit |
| Applications notes | CheKine™ Micro Superoxide anion Scavenging Assay Kit provides a simple and easy colorimetric assay for the study of Superoxide anion Scavenging ability in tissue/cell lysates and other biological fluids. In this assay, superoxide anion (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically. Sample scavenges the O2- thus less O2- is available for the chromogenic reaction. This scavenging ability of sample is measured by colorimetric method at OD 450 nm. |
| Kit components | • Assay Buffer • Sample Diluent • WST-8 • Enhancer • Xanthine Oxidase • Xanthine |
| Features & Benefits | • Determination the Superoxide anion Scavenging ability in various samples, including serum, plasma, plant tissue/cell lysates and other biological fluids. |
| Usage notes | • Briefly centrifuge small vials at low speed prior to opening. • If not assayed immediately, samples can be stored at -80°C. |
| Background | The superoxide anion (O2-) is a short-lived radical production by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation, or derived from oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components and causes damage to lipids, proteins and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders. The study of scavenging superoxide anion radicals has been gained more and more attention. |
CheKine™ 羥基自由基清除能力比色測定試劑盒 | CheKine™ Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit 貨號 KTB1091
羥基自由基(hydroxyl radical)•OH,是氫氧根離子(hydroxide ion , OH-),高反應性(容易變成羥基)。羥基自由基(Hydroxyl free radicals)作用於體內的蛋白質(proteins)、核酸(nucleic acids)、脂類(lipids)等生物分子,對細胞造成損害結構(structure)和功能(function),進而導致體內代謝紊亂(metabolic disorders)並引起疾病。羥基自由基清除能力(Hydroxyl free radical scavenging capacity)是樣品抗氧化能力的重要指標之一,已廣泛應用於抗氧化保健品(antioxidant health products)和藥物(drugs)的研究。 CheKine™ 羥基自由基清除能力比色測定試劑盒(CheKine™ Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit, KTB1091)專為檢測各種樣品中的羥基自由基清除能力(Hydroxyl Free Radical Scavenging Capacity)而開發。操作簡單方便,檢測更加靈敏準確。在該測定中,H2O2/ Fe2+ 產生羥基自由基(hydroxyl free radical)通過芬頓反應(Fenton reaction)。水楊酸(Salicylic acid)能有效捕捉生成的羥基自由基(hydroxyl free radical)並與之反應生成產生最大吸收峰在 520 nm 的 2,3-二羥基苯甲酸(2,3-dihydroxybenzoic acid)。在具有能力的物質之後清除羥基自由基,導致520nm吸光度下降。 520 nm 的吸光度值可以反映樣品的羥基自由基清除能力。
| Product name | CheKine™ Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit |
| Applications notes | Abbkine CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit (Colorimetric) (Salicylic Acid Method) is specially developed for the detection of Hydroxyl Free Radical Scavenging Capacity in various sample. The operation is simple and convenient, and the detection is more sensitive and accurate. In this assay, H2O2/ Fe2+ generates hydroxyl free radical through the Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl free radical and reacts with them to produce 2,3- dihydroxybenzoic acid with a maximum absorption peak at 520 nm. After the substances with the capacity to scavenge hydroxyl free radical, resulting in the decrease of 520 nm absorbance. The value of 520 nm absorbance can reflects the Hydroxyl Free Radical Scavenging Capacity of the sample. |
| Kit components | • Ferrous Salt • H2O2 •Salicylic Acid• Free-HSA Negative Control |
| Features & Benefits | • simple, sensitive, rapid detection method. • compatible to various biological samples such as plasma, serum,bacteria, animal /plant tissues and cells and other biological fluids. • Provides detailed sample preparation , results calculation methods and comprehensive results display. |
CheKine™ 過氧化氫 (H2O2) 檢測試劑盒 | CheKine™ Micro Hydrogen Peroxide (H2O2) Assay Kit貨號KTB1041
過氧化氫 (Hydrogen Peroxide , H2O2) 是一種活性氧(reactive oxygen)代謝副產物,它既是細胞內信號(intracellular signaling messenger)傳遞的信使,也是氧化應激(oxidative stress)的來源。過氧化氫(Hydrogen peroxide)通過多種機制在細胞中產生,例如由中性粒細胞(neutrophils)和巨噬細胞(macrophages)(respiratory burst, 呼吸爆發)產生 NOX 介導的 ROS(NOX-mediated ROS),或通過線粒體呼吸過程中電子洩漏(electron leak)產生的超氧陰離子歧化酶(superoxide anions)產生。通過 NF-κB 和其他因子發揮作用,氫過氧化物介導(hydroperoxide-mediated pathways)的途徑與哮喘(asthma)、炎症性關節炎(inflammatory arthritis)、動脈粥樣硬化(atherosclerosis)、糖尿病性血管病(diabetic vasculopathy)、骨質疏鬆症(osteoporosis)、神經退行性疾病(neurodegenerative diseases)、唐氏綜合症(Down’s syndrome)和免疫系統疾病(immune system diseases)有關。CheKine™ 微量過氧化氫檢測試劑盒(CheKine™ Micro Hydrogen Peroxide Assay Kit, KTB1041)提供了一種簡單易用的比色檢測方法,用於測量血清、血漿、細胞培養上清液、組織/細胞裂解物和其他生物體液中的過氧化氫(hydrogen peroxide)。在該測定中,H2O2 將亞鐵(ferrous)氧化為鐵離子(ferric ion),二甲酚橙(xylenol orange)以高選擇性與鐵離子結合形成有色(紫色)產物,可在 OD 580 nm 處通過比色法測量。樣品中存在的過氧化氫過氧化氫 (Hydrogen Peroxide , H2O2)與獲得的信號成比例。
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
| Product name | CheKine™ Micro Hydrogen Peroxide (H2O2) Assay Kit |
| Kit components | • Reaction Buffer • H2O2 Standard (1M) • Assay Buffer (10X) |
| Features & Benefits | • Determination of Hydrogen Peroxide in serum, plasma, plant or animal tissue samples, urine and other biological fluids. • Simple procedures, fase and convenient, and more stable. |
| Usage notes | • It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range. • Fresh samples are necessary for good results. If not assayed immediately, samples can be stored at -80°C for one month. |
CheKine™ 超氧陰離子比色測定試劑盒 | CheKine™ Superoxide Anion Colorimetric Assay Kit貨號KTB1210
absorption peak at 540nm
0.0125-0.5 mg/mL
生物體內的超氧陰離子(Reactive oxygen species)等活性氧具有免疫(immune)和信號轉導作用(signal transduction effects),但當它們積累過多時,會破壞細胞膜(cell membranes)和生物大分子(biological macromolecules),導致體內細胞和組織的代謝異常(abnormal metabolism),引起多種疾病。該試劑盒提供了一種檢測動物組織、植物組織、血清、細胞等多種生物樣品中O2-含量的簡便方法。測定中,多種生物樣品中的超氧陰離子(superoxide anion)與鹽酸羥胺(hydroxylamine hydrochloride)反應生成NO2-,NO2-與Gris試劑(Gris’ reagent)反應。 Gris分析的機理概括為重氮之間的偶氮偶聯,即磺胺類(sulfonamides)與NO2-和N-(1-萘基)乙二胺二鹽酸鹽生成紅色偶氮化合物,在540nm有特徵吸收峰。樣品中的 O2- 含量可根據 A540 值計算。
| Product name | CheKine™ Superoxide Anion Colorimetric Assay Kit |
| Kit components | • Extraction Buffer • Reagent I • Reagent II • Reagent III • NaNO2 Standard (10mmoL/L) |
| Features & Benefits | • Determination of the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. • Standard and standard curve line are provided. |
| Calibration range | 0.0125-0.5 mg/mL |
| Limit of detection | 0.0125 mg/mL |
CheKine™ 超氧陰離子比色測定試劑盒 | CheKine™ Superoxide Anion Colorimetric Assay Kit貨號KTB1210
absorption peak at 540nm
0.0125-0.5 mg/mL
生物體內的超氧陰離子(Reactive oxygen species)等活性氧具有免疫(immune)和信號轉導作用(signal transduction effects),但當它們積累過多時,會破壞細胞膜(cell membranes)和生物大分子(biological macromolecules),導致體內細胞和組織的代謝異常(abnormal metabolism),引起多種疾病。該試劑盒提供了一種檢測動物組織、植物組織、血清、細胞等多種生物樣品中O2-含量的簡便方法。測定中,多種生物樣品中的超氧陰離子(superoxide anion)與鹽酸羥胺(hydroxylamine hydrochloride)反應生成NO2-,NO2-與Gris試劑(Gris’ reagent)反應。 Gris分析的機理概括為重氮之間的偶氮偶聯,即磺胺類(sulfonamides)與NO2-和N-(1-萘基)乙二胺二鹽酸鹽生成紅色偶氮化合物,在540nm有特徵吸收峰。樣品中的 O2- 含量可根據 A540 值計算。
| Product name | CheKine™ Superoxide Anion Colorimetric Assay Kit |
| Kit components | • Extraction Buffer • Reagent I • Reagent II • Reagent III • NaNO2 Standard (10mmoL/L) |
| Features & Benefits | • Determination of the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. • Standard and standard curve line are provided. |
| Calibration range | 0.0125-0.5 mg/mL |
| Limit of detection | 0.0125 mg/mL |
NAD/NADH 分析試劑盒| CheKine™ NAD/NADH Assay Kit貨號KTB1020
煙醯胺腺苷二核苷酸(Nicotinamide adenine dinucleotide , NAD)是一種酶共體(enzymatic cofactor),涉及許多氧化還原反應(redox reactions)。NAD作為電子載體(redox reactions),在氧化 (NAD) 和還原 (NADH) 形式之間迴圈。NAD 除了在紅氧反應中的作用外,在 ADP-核糖酸反應中扮演重要的作用,並且作為sirtuins的substrate。Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP-ribosylation reactions and as a substrate for sirtuins.

Fig. Standard Curve of NAD in 96-well plate assay. The y-axis is ΔOD and the x-axis is NAD concentration (uM).
Abbkine CheKine™ NAD/NADH 分析試劑盒(Abbkine CheKine™ NAD/NADH Assay Kit, 貨號KTB1020), 檢測各種組織和細胞各種胞器中NAD 和 NADH比例。此試劑盒基於酶循環反應 (它不辨認 NADP+/NADPH),其中形成的 NADH 減少了 formazan (MTT) 。以波長 OD565 nm 下測量,吸收光的強度與樣品中的 NAD+ /NADH 濃度成正比。 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。
| it components | • Assay Buffer • EtOH Solution • WST-8 Solution • Enhancer • NAD Cycling Enzyme Mix • NAD standard (10 mM) • NAD Extraction Buffer • NADH Extraction Buffer |
| Features & Benefits | • Detect NAD+/NADH concentrations and ratios in cell or tissue extracts. • Detection limit of 0.78 µM and linearity up to 50 µM NAD+/NADH in 96-well plate assay. |
| Calibration range | 0.78 µM-50 µM |
| Usage notes | • Addition of Working Reagent should be quick and mixing should be brief but thorough. • The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%). |
| Storage instructions | Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
DNA Damege損傷/細胞Stress
| 產品貨號 | 產品品項 | 反應 | 測量儀器 |
| 貨號KTB1050 | 脂質過氧化檢測試劑盒(MDA) | 96個反應 |
OD 532 nm 1-100 µM/L |
| 貨號KTB1910 | 活性氧(ROS)檢測分析試劑盒 | 50個反應 | DCFH-DA (10 mM) |
| 貨號KTB1400 | 一氧化Dan(NO)檢測試劑盒 | 48個反應 |
colorimetric (540 nm) 檢測範圍:1-100 µM |
| 貨號KTB1080 | 超氧陰離子清除測定試劑盒 | 48個反應 | Superoxide anion scavenging rate D% =(∆∆ODControl – ∆∆ODSample)÷ ∆∆ODControl ×100% |
| 貨號 KTB1091 | 羥基自由基清除能力比色測定試劑盒 | 48個反應 | 吸收光 520nm |
| 貨號KTB1041 | CheKine™ 過氧化氫 (H2O2) 檢測試劑盒 | 96個反應 |
OD 580 nm : 1 µM-100 µM |
| 貨號KTB1210 | CheKine™ 超氧陰離子比色測定試劑盒 | 48個反應 |
absorption peak at 540nm 0.0125-0.5 mg/mL |
| 貨號KTB1020 | NAD/NADH 分析試劑盒 | 48個反應 |
OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。 |



