細胞代謝檢測試劑盒 (Cell metabolism detection kit)- ABTS 自由基清除能力檢測試劑盒

ABTS 自由基清除能力檢測試劑盒 | ABTS Free Radical Scavenging Capacity Assay Kit Abbkine貨號KTB1093

CheKine™ Micro ABTS Free Radical Scavenging Capacity Assay Kit

產品介紹:

總抗氧化能力(The total antioxidant)由被測物中各種抗氧化物質(antioxidant substances )和抗氧化酶(antioxidant enzymes )組成。 ABTS法 (ABTS method) 是應用最廣泛的間接檢測方法(indirect detection method)，適用於測定親水性(hydrophilic)和親脂性物質(lipophilic substances)的抗氧化能力。 CheKine™ Micro ABTS自由基清除能力檢測試劑盒 (CheKine™ Micro ABTS Free Radical Scavenging Capacity Assay Kit) 提供了簡單、快速的ABTS自由基清除活性測定方法，適用於植物組織 (plant tissues)、紅酒 (red wine)等液體樣本。其原理是ABTS被氧化生成穩定的藍綠色陽離子自由基ABTS+，ABTS+可溶於水或酸性乙醇介質，並在734 nm處具有最大吸收。將待測物質加入ABTS+溶液後，樣本中的抗氧化成分與ABTS+反應，導致反應體系褪色。在734 nm處測量吸光度的變化，並透過與維生素C參考體系進行比較來量化待測物質的抗氧化能力。

樣品製備: 

注意：我們建議您使用新鮮樣品。如不立即進行檢測，樣本可在-80℃下保存一個月。

1. 植物樣品製備：秤取約0.1 g樣品，加入1 mL萃取緩衝液，冰上勻漿。 4℃下以10,000 g離心10 min，收集上清液，置於冰上保存以備後續分析。

2. 液體樣品（如紅酒、果汁等）：吸取100 μL樣品溶液加入900 μL萃取緩衝液中，渦旋混勻。 4℃下以10,000 g離心10 min，收集上清液，置於冰上保存以備後續分析。

檢測步驟

1. 將微孔板讀數儀或可見分光光度計預熱30分鐘以上，將波長調至734nm，用去離子水將可見分光光度計歸零。 2. 操作步驟（以下操作皆在96孔板或微型玻璃比色皿中進行）：

註：陽性對照孔和空白孔只需測量1-2次。不同樣品清除ABTS自由基的能力可能有顯著差異。建議在進行正式實驗前，先用2-3個預期差異較大的樣品進行預實驗。如果清除率低於5%，可以適當增加樣品量，例如增加液體樣品的體積。如果清除率超過90%，可以用萃取緩衝液進一步稀釋樣品，並將計算結果乘以稀釋倍數，或減少萃取所需的樣品量。本譯文保留了原文的技術細節和上下文，確保實驗步驟清晰易懂。

Product name	CheKine™ Micro ABTS Free Radical Scavenging Capacity Assay Kit
SampleType	Plant Tissues, Red Wine, and other Liquid samples
Alternative	ABTS Free Radical Scavenging Capacity
Kit components	• Extraction Buffer •ReagentⅠ •Vitamin C (Positive Control)
Features & Benefits	The verified samples are complete in type and easy to operate．
Usage notes	• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don’t perform the assay at the same time, it’s better to complete the Sample Preparation step before storing the samples.
Storage instructions	Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.