【ROS活性氧檢測分析】Reactive Oxygen Species Detection Assay Kit貨號KTB1910
CheKine™ Reactive Oxygen Species (ROS) Detection Assay Kit (Fluorometric) 10 mM 50個反應/100個反應 (以6well, 1ml體積為一個反應)
CheKine™活性氧（CheKine™ Reactive Oxygen Species , ROS）檢測分析試劑盒提供了一種簡單，靈敏，快速的ROS檢測方法。 ROS檢測基於螢光探針DCFH-DA。 DCFH-DA是一種可滲透細胞的靈敏探針，用於檢測細胞內活性氧（reactive oxygen species , ROS）。 該DCFH-DA探針可在穿過活細胞膜時被細胞中的酯酶水解為DCFH。 DCFH沒有螢光並且不能透過細胞膜，然後可以被ROS氧化並產生螢光DCF。 然後可以通過流式細胞儀或螢光顯微鏡檢測螢光，並據此分析細胞中活性氧（CheKine™ Reactive Oxygen Species , ROS）的含量。
活性氧物種(Reactive Oxygen Species, Ros ) Ros是氧正常代謝的天然副產物，在細胞信號傳導(cell signaling)和體內平衡(vivo balance)中起重要作用。 但是，在氧化應激相關狀態(oxidative stress correlation state)下，ROS水平可以大大提高。 ROS的積累將嚴重破壞細胞結構(cell structure)。 氧化應激(Oxidative stress)在心血管疾病(cardiovascular disease)，糖尿病(diabetes)，骨質疏鬆(osteoporosis)，中風(stroke)，炎性疾病(inflammatory disease)以及神經退行性疾病(neurodegenerative diseases)和癌症(cancer)等研究中起著重要作用。 ROS檢測將有助於確定氧化應激(oxidative stress)如何調節各種細胞內途徑(intracellular pathways)。Reactive Oxygen Species, Ros is natural by-products of oxygen normal metabolism, play an important role in cell signaling and in vivo balance. However, in the oxidative stress correlation state, the ROS level can be greatly increased. The accumulation of ROS will seriously damage the cell structure. Oxidative stress plays an important role in the research of cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory disease, and neurodegenerative diseases and cancer,etc. ROS detection will help determine how oxidative stress adjusts various intracellular pathways.
| Kit components | • DCFH-DA (10 mM) |
| Features & Benefits | • provides a simple, sensitive, rapid ROS detection method. • based on the fluorescent probe DCFH-DA . • suitable for flow cytometry or fluorescence microscopy. • Provides detailed sample preparation and results calculation methods. |
| Usage notes | • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • The working concentration of positive control H2O2 should be 100 μM (Recommend during 50-200 μM depend on the cell types ). The level of Reactive Oxygen Species (ROS) will increase significantly after stimulated 30 min-4 h. The level of Reactive Oxygen Species (ROS) maybe very different for different cells while stimulating with the positive control. The stimulating time and the concentration of positive control could be prolonged or increased, if there is no obvious rise after 30 min . Or shorten the time or reduce the concentration of positive control, while the fluorescent signal is too strong. • During the experiment, the fluorescent probe DCFH-DA can be diluted in 1: 2000-1: 5000, and make the final concentration of DCFH-DA to 2-5 μM if the signal of negative control also too strong. The time of probe incubating can be adjusted in 15-60 minutes, and minimize the exposure time while reading the film, and the exposure time of the control group and test groups should to be consistent. • Reactive Oxygen Species (ROS) positive control (H2O2) is only used as a sample as a positive control, and not all test groups need positive control. • Please wash away the unbound prode after incubating the prode , otherwise it will cause a high background. • Try to shorten the detection time after incubating the probe (except for stimulation time) to reduce various possible errors. |
| Storage instructions | Please store the whole kit at -20°C upon receipt ,and protect from light. The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
引用文獻
LncRNA SNHG4 regulates miR-10a/PTEN to inhibit the proliferation of acute myeloid leukemia cells(KTP3006-1.472)作者：Yuan Z, Wang W. 雜誌名稱：Hematology IF：1.472
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
BRAND/AGENCY/台灣代理商/ Abbkine台灣代理商
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| 貨號 | 產品名稱 | 胞器染色位置 | 螢光波段 | |
| 貨號KTC4003 | TraKine™粒線體染色 | 粒線體(mitochondria) | Ex / Em = 490/523 nm | 增殖(proliferating)和非增殖細胞(non-proliferating cells) |
| 貨號KTC4100 | Pro活細胞微管蛋白染色 | 微管蛋白(Tubulin) | Ex/Em = 500/520 nm | 活細胞或固定之細胞 |
| 貨號KTC4001 | Abbkine 細胞膜染色 | 細胞膜 (plasma membrane) | Ex/Em = 484/501 nm | 活細胞、固定懸浮、貼附細胞 |
| 貨號BMD00063 | DAPI Staining Solution | 細胞核 dsDNA | Ex/Em: 358/461 nm (with DNA) | 染色活細胞和固定細胞 |
| 貨號KTC4210 | 活細胞Lysosome染色 | Lysosome | deep red fluorescent dye , Ex/Em:650/665nm | 染色活細胞和固定細胞 |