【生長因子/細胞激素】Growth factors & cytokines-CHO cell/HEK293/E.coli表現系統
【生長因子與細胞激素】 CHO cell/HEK293 /E.coli表現系統
Growth factors生長因子和cytokines細胞因子，是天然存在的signaling molecules信號分子，它們是高等生物細胞之間攜帶調節信號的關鍵因素。儘管細胞因子主要與immune system housekeeping免疫系統相關，生長因子在生物體的發育和成熟階段調節具廣泛的過程；不受控制的生長因子激活通常與oncogenesis腫瘤發生相關。生長因子和細胞因子對於細胞的許多功能是不可缺少的。傳統的細胞生物學實驗使用動物血清作為正常細胞代謝所需的生長因子和營養來源。但是，使用動物血清，容易增加病毒，支原體和朊病毒污染的風險；與血清收集相關的高成本和倫理問題；對可能還含有生長抑制劑。高品質Growth factors&cytokines產品具有以下特點：(A) 嚴格條件下的高純度生產確保質量保持一致，每批批次純度≥95％通過SDS-PAGE. (B) 內毒素最小 – Limulus Amebocyte Lysate (LAL) assay。低於1 EU/ug recombinant protein。(C) 確保每一批生物活性。
aFGF重組蛋白 | 293細胞表達Growth Factors貨號 Z100005
aFGF, FGF-1, Heparin-binding growth factor 1, HBGF-1, Endothelial cell growth factor, ECGF-beta, FGFA, FGF1
產品介紹: aFGF和bFGF顯示55％的同源性，然而限於幾個功能域。 通過X射線晶體學顯示的aFGF的結構顯示出與IL1相似的折疊結構。 因為aFGF與bFGF相同的受體結合，所以aFGF或多或少顯示相同的活性譜。然而，在類似的測定中，它通常比bFGF活性低。 FGF受體由至少四種受體酪氨酸激酶組成的基因家族編碼，其轉導在與細胞生長和分化相關的各種發育和生理過程中重要的信號。 重組人FGF1是16kDa的單一多肽。
aFGF重組蛋白 | IL-2重組蛋白 | 幹細胞因子（KITLG） | 重組人白血病抑制因子（LIF） | 重組人Human Noggin (NOG) | TNF-a | BMP2
| Gene Symbol | FGF1 |
| Gene ID | 9158 |
| Accession No. | P05230 |
| Source | E. coli |
| Appearance | Lyophilized Powder |
| Molecular Weight | 16.0 kDa |
| Endotoxin Level | <1.0 EU/μg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Gel Image | |
| Bioactivity | The ED50 as determined by the dose-dependent stimulation of mouse fibroblasts was <0.5ng/ml |
| Bioactivity Data | |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Product | Source | Size | Cat. No. |
| Recombinant Human FGF1 | E. coli | 50 µg | Z100005 |
| Recombinant Mouse FGF1 | E. coli | 50 µg | Z200005 |
| Recombinant Human FGF10 | E. coli | 25 µg | Z100205 |
| Recombinant Human FGF16 | E. coli | 20 µg | Z102295 |
| Recombinant Human FGF17 | E. coli | 20 µg | Z102305 |
| Recombinant Human FGF18 | E. coli | 20 µg | Z102315 |
| Recombinant Human FGF19 | E. coli | 25 µg | Z102325 |
| Recombinant Human FGF10 | E. coli | 1.0 mg | Z100209 |
| Recombinant Human FGF1 | E. coli | 1.0 mg | Z100009 |
| Recombinant Mouse FGF1 | E. coli | 1.0 mg | Z200009 |
| Recombinant Human FGF17 | E. coli | 1.0 mg | Z102309 |
| Recombinant Human FGF19 | E. coli | 1.0 mg | Z102329 |
| Recombinant Human FGF16 | E. coli | 1.0 mg | Z102299 |
| Recombinant Human FGF1 | E. coli | 250 µg | Z100007 |
| Recombinant Human FGF10 | E. coli | 100 µg | Z100207 |
| Recombinant Human FGF16 | E. coli | 100 µg | Z102297 |
| Recombinant Human FGF17 | E. coli | 100 µg | Z102307 |
| Recombinant Human FGF18 | E. coli | 200 µg | Z102317 |
| Recombinant Human FGF18 | E. coli | 1.0 mg | Z102319 |
| Recombinant Mouse FGF1 | E. coli | 200 µg | Z200007 |
IL-2重組蛋白 | 293細胞表達Growth Factors貨號 Z102877
IL-2, T-cell growth factor, TCGF Recombinant Human Interleukin-2 (IL2)
產品介紹: 4個α螺旋的螺旋束，IL2是由CD4 + / CD8 + T細胞，γδT細胞，B細胞，嗜酸性粒細胞和樹突狀細胞表達的免疫調節細胞因子。 IL2刺激B細胞，NK細胞，淋巴細胞激活的殺傷細胞，單核細胞，巨噬細胞和少突膠質細胞的生長和分化。 雖然人IL2分別與小鼠和大鼠IL2分別具有56％和66％的氨基酸序列同一性，但只有人和小鼠IL2表現出跨物種活性。 特別地，IL2在T細胞上發揮自分泌和旁分泌活性。 在調節作用中，IL2對於調節性T細胞的發育，存活和功能是重要的。 它增強Fas介導的活化誘導的細胞死亡，並抑制炎性Th17細胞的發育。 重組人IL2在人293細胞中以分子量為15kDa的單體糖蛋白表達。
| Gene Symbol | IL2 |
| Gene ID | 3558 |
| Accession No. | P60568 |
| Source | HEK293 |
| Appearance | Lyophilized Powder |
| Molecular Weight | 15 kDa, monomer, glycosylated |
| Endotoxin Level | <1 EU/µg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Bioactivity | The EC(50) as determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line) was found to be ≤5 ng/mL. |
| Formulation | 1x PBS |
| Product | Source | Size | Cat. No. |
| Recombinant Human IL2 | HEK293 | 10 µg | Z102875 |
| Recombinant Human IL2 | HEK293 | 100 µg | Z102877 |
| Recombinant Human IL37 (IL1F7) | HEK293 | 10 µg | Z102885 |
| Recombinant Human IL37 (IL1F7) | HEK293 | 100 µg | Z102887 |
| Recombinant Human IL27 | HEK293 | 10 µg | Z102895 |
| Recombinant Human IL27 | HEK293 | 100 µg | Z102897 |
| Recombinant Human IL28A | HEK293 | 10 µg | Z102905 |
| Recombinant Human IL28A | HEK293 | 100 µg | Z102907 |
| Recombinant Human IL28B (IFNL3) | HEK293 | 10 µg | Z102915 |
| Recombinant Human IL28B (IFNL3) | HEK293 | 100 µg | Z102917 |
| Recombinant Human IL29 (IFNL1) | HEK293 | 10 µg | Z102925 |
| Recombinant Human IL29 (IFNL1) | HEK293 | 100 µg | Z102927 |
| Recombinant Human IL2 | E. coli | 50 µg | Z100485 |
| Recombinant Mouse IL2 | E. coli | 20 µg | Z200135 |
| Recombinant Human IL21 | E. coli | 10 µg | Z100495 |
| Recombinant Human IL29 (IFNL1) | E. coli | 20 µg | Z100505 |
| Recombinant Human IL22 | E. coli | 10 µg | Z100945 |
| Recombinant Mouse IL25 | E. coli | 10 µg | Z200255 |
| Recombinant Human IL20 | E. coli | 10 µg | Z101075 |
| Recombinant Rat IL20 | E. coli | 10 µg | Z300165 |
| Recombinant Human IL2 | CHO cells | 50 µg | Z101235 |
| Recombinant Human IL21 | CHO cells | 20 µg | Z101245 |
| Recombinant Human IL22 | CHO cells | 20 µg | Z101255 |
| Recombinant Human IL24 | CHO cells | 10 µg | Z101265 |
| Recombinant Human IL27 | CHO cells | 100 µg | Z101275 |
| Recombinant Human IL28B (IFNL3) | CHO cells | 10 µg | Z101285 |
| Recombinant Human IL37 (IL1F7) | E. coli | 10 µg | Z102085 |
| Recombinant Rat IL20 | E. coli | 1.0 mg | Z300169 |
| Recombinant Mouse IL2 | E. coli | 1.0 mg | Z200139 |
| Recombinant Human IL2 | E. coli | 1.0 mg | Z100489 |
| Recombinant Human IL29 (IFNL1) | E. coli | 1.0 mg | Z100509 |
| Recombinant Mouse IL25 | E. coli | 1.0 mg | Z200259 |
| Recombinant Human IL22 | E. coli | 1.0 mg | Z100949 |
| Recombinant Human IL21 | E. coli | 1.0 mg | Z100499 |
| Recombinant Human IL20 | E. coli | 1.0 mg | Z101079 |
| Recombinant Human IL37 (IL1F7) | E. coli | 1.0 mg | Z102089 |
| Recombinant Human IL28A | E. coli | 20 µg | Z102455 |
| Recombinant Human IL28A | E. coli | 1.0 mg | Z102459 |
| Recombinant Rat IL2 | E. coli | 5 µg | Z300195 |
| Recombinant Rat IL2 | E. coli | 1.0 mg | Z300199 |
| Recombinant Human IL2 | E. coli | 100 µg | Z100487 |
| Recombinant Human IL21 | E. coli | 100 µg | Z100497 |
| Recombinant Human IL22 | E. coli | 100 µg | Z100947 |
| Recombinant Mouse IL2 | E. coli | 100 µg | Z200137 |
| Recombinant Rat IL2 | E. coli | 100 µg | Z300197 |
| Recombinant Human IL37 (IL1F7) | E. coli | 100 µg | Z102087 |
| Recombinant Mouse IL25 | E. coli | 100 µg | Z200257 |
TNF-a Growth Factors and Cytokines | 293細胞表達Growth Factors貨號Z103077
TNF, TNFA, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a
產品介紹: 巨噬細胞，單核細胞，嗜中性粒細胞，T細胞，細胞脂多醣刺激後的自然殺傷細胞分泌腫瘤壞死因子。 刺激的外周嗜中性粒細胞，還有未刺激的細胞，還有許多轉化的細胞系，星形膠質細胞，小膠質細胞，平滑肌細胞和成纖維細胞也分泌TNF。 人乳也含有這個因素。 表達CD4分泌TNF-α的細胞，而CD8（+）細胞分泌很少或不分泌TNF-α。 TNF-α的合成由包括干擾素，IL-2，GM-CSF，SP，緩激肽，免疫複合物，環加氧酶抑製劑和PAF（血小板活化因子）在內的許多不同刺激物誘導。 已顯示在人293細胞中表達的重組人TNF主要是非共價連接的同三聚物，分子量為51kDa。
| Gene Symbol | TNF |
| Gene ID | 7124 |
| Accession No. | P01375 |
| Source | HEK293 |
| Appearance | Lyophilized Powder |
| Molecular Weight | 17 kDa, non-disulfide bonded homotrimer, glycosylated |
| Endotoxin Level | <1 EU/µg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Bioactivity | The EC(50) as determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D was found to be ≤ 1 ng/mL. |
| Formulation | 1x PBS |
| Reconstitution | A quick spin of the vial followed by reconstitution in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
BMP2 Growth Factors and Cytokines | 293細胞表達Growth Factors貨號Z102667
產品介紹: BMPs是起始骨形成之前誘導間充質細胞分化為軟骨細胞和成骨細胞的蛋白質。 它們促進骨折位置附近的軟骨形成細胞和骨形成細胞的分化，但也在異位的位置。 作為有效的骨誘導性細胞因子，BMP-2能夠與骨傳導載體（如膠原蛋白和合成羥基磷灰石）相關聯地誘導骨和軟骨形成。 BMP-2野生型通過兩個不同的結合表位與其細胞受體結合。 大表位1負責與BMPR-IA受體的高親和力結合，較小的表位2提供與受體BMPR-II的低親和力結合。 在人293細胞中表達的BMP-2提供了真實的糖基化，其有助於細胞生長培養基和其它應用中的穩定性。 由人293細胞表達的BMP-2是分子質量為28kDa的二硫鍵連接的同型二聚體糖蛋白。
| Gene Symbol | BMP2 |
| Gene ID | 650 |
| Accession No. | P12643 |
| Source | HEK293 |
| Appearance | Lyophilized Powder |
| Molecular Weight | 28 kDa, disulfide linked homodimeric |
| Endotoxin Level | <1 EU/µg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Bioactivity | The EC(50) as determined by the dose dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line) was found to be ≤ 60 ng/mL |
| Formulation | 2x PBS + 6% Ethanol |
| Reconstitution | A quick spin of the vial followed by reconstitution in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
| Product | Source | Size | Cat. No. |
| Recombinant Human BMP2 | HEK293 | 10 µg | Z102665 |
| Recombinant Human BMP2 | HEK293 | 100 µg | Z102667 |
| Recombinant Human BMP2 | E. coli | 10 µg | Z100085 |
| Recombinant Human BMP2 | E. coli | 1.0 mg | Z100089 |
重組人Human Noggin (NOG) (HEK293) | 貨號Z101345
產品介紹: Noggin是分泌的同型二聚體糖蛋白，其結合TGF-β家族（BMP）的配體並通過抑制它們進入信號受體來調節其活性。 成熟的人Noggin蛋白質含有N-末端酸性區域，中央鹼性肝素結合區段和C末端半胱氨酸結結構。 Noggin結合具有可變親和力的不同BMPs，在骨骼發育過程中拮抗特異性BMPs。 在成體中樞神經系統和外周組織如肺，骨骼肌和皮膚的限定區域中表達Noggin。 在人293細胞中表達的重組人Noggin是分子量為65kDa的糖基化二硫鍵連接的同二聚體。
| Gene Symbol | NOG |
| Gene ID | 9241 |
| Accession No. | Q13253 |
| Source | HEK293 |
| Appearance | Lyophilized Powder |
| Molecular Weight | 65 kDa, homodimer, glycosylated |
| Endotoxin Level | <1 EU/µg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Bioactivity | The EC(50) as determined by the dose-dependent inhibition of rh-BMP-4 induced alkaline phosphate production by ATDC5 cells was found to be ≤ 25 ng/mL. |
| Formulation | 1x PBS |
| Product | Source | Size | Cat. No. |
| Recombinant Human Noggin (NOG) | HEK293 | 10 µg | Z102995 |
| Recombinant Human Noggin (NOG) | HEK293 | 100 µg | Z102997 |
| Recombinant Human Noggin (NOG) | E. coli | 20 µg | Z100925 |
| Recombinant Human Noggin (NOG) | CHO cells | 50 µg | Z101355 |
| Recombinant Mouse Noggin (NOG) | E. coli | 1.0 mg | Z200239 |
| Recombinant Human Noggin (NOG) | E. coli | 1.0 mg | Z100929 |
重組人白血病抑制因子（LIF） (CHO Cell) | 貨號Z101345
LIF、分化刺激因子、D因子、黑素瘤來源的LPL抑制劑、MLPLI、INN = Emfilermin，HILDA、重組人白血病抑制因子（LIF）
產品介紹: LIF是以可溶性和基質結合形式存在的多功能分泌型糖蛋白。 它顯示生物活性，從骨髓白血病細胞分化為巨噬細胞譜系，對骨代謝，炎症，神經發育，胚胎髮生和植入維持的影響。 現在清楚的是，LIF在結構和作用機制上都與細胞因子白細胞介素IL-6家族有關，細胞因子也包括IL-11，睫狀神經營養因子，制瘤素M和心肌營養因子1.這些細胞因子的作用介導 通過特定的細胞表面受體由獨特的鍊和共享的信號轉導子單元gp130組成。
| Gene Symbol | LIF |
| Gene ID | 3976 |
| Accession No. | P15018 |
| Source | CHO cells |
| Appearance | Lyophilized Powder |
| Molecular Weight | 20.0 kDa |
| Endotoxin Level | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Purity | >95% as determined by SDS-PAGE |
| Bioactivity | The ED(50) was determined by the dose-dependent differentiation of M1 myeloid leukemic cells was found to be in the range of 0.01 ng/mL. |
| Formulation | Recombinant Leukemia Inhibitory Factor was lyophilized from a 0.2 μm filtered PBS pH 4.5 |
| Product | Source | Size | Cat. No. |
| Recombinant Human THPO | HEK293 | 10 µg | Z103085 |
| Recombinant Human THPO | HEK293 | 100 µg | Z103087 |
| Recombinant Human LIF | E. coli | 10 µg | Z100605 |
| Recombinant Mouse LIF | E. coli | 10 µg | Z200195 |
| Recombinant Human SERPINF1 | E. coli | 10 µg | Z100765 |
| Recombinant Human MIF | E. coli | 25 µg | Z100915 |
| Recombinant Mouse LIF | CHO cells | 100 µg | Z200485 |
| Recombinant Human LIF | CHO cells | 50 µg | Z101345 |
| Recombinant Human THPO | E. coli | 10 µg | Z101515 |
| Recombinant Mouse THPO | E. coli | 10 µg | Z200535 |
| Recombinant Mouse LIF | E. coli | 1.0 mg | Z200199 |
| Recombinant Human MIF | E. coli | 1.0 mg | Z100919 |
| Recombinant Human LIF | E. coli | 1.0 mg | Z100609 |
| Recombinant Human SERPINF1 | E. coli | 1.0 mg | Z100769 |
| Recombinant Human THPO | E. coli | 1.0 mg | Z101519 |
| Recombinant Mouse THPO | E. coli | 1.0 mg | Z200539 |
| Recombinant Human LIF | E. coli | 100 µg | Z100607 |
| Recombinant Human MIF | E. coli | 100 µg | Z100917 |
| Recombinant Human SERPINF1 | E. coli | 500 µg | Z100767 |
| Recombinant Mouse LIF | E. coli | 100 µg | Z200197 |
| Recombinant Porcine LIF | E. coli | 10 µg | Z400015 |
| Recombinant Porcine LIF | E. coli | 1.0 mg | Z400019 |
| Recombinant Porcine LIF | E. coli | 100 µg | Z400017 |
幹細胞因子（KITLG）生長因子和細胞因子 (CHO Cell) | 貨號Z200505
產品介紹: 幹細胞因子（KITLG）生長因子和細胞因子，同義字SCF, Kit ligand, c-Kit ligand, Hematopoietic growth factor KL, Mast cell growth factor, MGF, Steel factor。SCF是由成纖維細胞和其他細胞類型合成的基質細胞來源的細胞因子。SCF促進多能幹細胞水平的細胞增殖和早期分化。已經提出SCF對於各種造血譜系的最佳生產是必不可少的，主要是因為其與其它細胞因子共同刺激時能夠預防細胞凋亡。 稱為SCFR（CD117）的SCF受體是指定為KIT的致癌基因。 SCF的生物活性通過集落刺激因子GM-CSF和G-CSF以及IL-7，Epo等一些生長和分化因子相互協同。 結合IL-7 SCF刺激前B細胞的增殖。 SCF也是細胞的有效化學引誘物（參見：趨化性），例如表達試劑盒受體的肥大細胞。 這些細胞中對SCF的一個反應是細胞骨架的肌動蛋白絲的特徵重排。
| Gene Symbol | KITLG |
| Gene ID | 17311 |
| Accession No. | P20826 |
| Source | CHO cells |
| Appearance | Lyophilized Powder |
| Molecular Weight | 18.0 kDa |
| Endotoxin Level | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Purity | >95% as determined by SDS-PAGE |
| Bioactivity | The ED(50) was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells to be < 2.0 ng/mL, corresponding to a specific activity of > 5.0 x 10^5 units/mg. |
| Formulation | Recombinant Stem cell factor was lyophilized from a 0.2 μm filtered solution in 20mM Tris, 5% trehalose, pH 7.5. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Product | Source | Size | Cat. No. |
| Recombinant Human Stem Cell Factor (KITLG) | HEK293 | 10 µg | Z103035 |
| Recombinant Human Stem Cell Factor (KITLG) | HEK293 | 100 µg | Z103037 |
| Recombinant Human Stem Cell Factor (KITLG) | E. coli | 10 µg | Z100815 |
| Recombinant Mouse Stem Cell Factor (KITLG) | CHO cells | 100 µg | Z200505 |
| Recombinant Human Stem Cell Factor (KITLG) | CHO cells | 25 µg | Z101375 |
| Recombinant Mouse Stem Cell Factor (KITLG) | E. coli | 10 µg | Z200595 |
| Recombinant Human Stem Cell Factor (KITLG) | E. coli | 1.0 mg | Z100819 |
| Recombinant Mouse Stem Cell Factor (KITLG) | E. coli | 1.0 mg | Z200599 |
| Recombinant Human Stem Cell Factor (KITLG) | E. coli | 100 µg | Z100817 |
| Recombinant Mouse Stem Cell Factor (KITLG) | E. coli | 100 µg | Z200597 |