Ki-67單株抗體 | 貨號ABM40064

細胞增殖(Cell proliferation)可用於評估正常的細胞健康狀況(normal cell health)，測量對毒性損傷(toxic insult)的反應，或用作幾種癌症的預後(prognostic)和診斷工具(diagnostic tool)。可用的標記通常著重於DNA水平(DNA levels)或合成(synthesis)，細胞代謝(cellular metabolism)或增殖特異性蛋白(proliferation-specific proteins)。

產品描述： Ki-67是一種由人MKI67基因編碼的蛋白質。該蛋白與細胞的增殖密切相關。MKI67編碼核蛋白(nuclear protein)（增殖Ki-67的標記）與細胞增殖(cellular proliferation)相關。Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

Fig.1. IHC-P staining of mouse kidney tissue, diluted at 1:200.

Fig.2. Immunofluorescence analysis of human breast cancer tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of mouse testis tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunofluorescence analysis of rat brain tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.5. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, Ki 67 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Ki 67 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

• Product name

Ki 67 Monoclonal Antibody

• Immunogen

Synthetic Peptide

• Host

Mouse

• Reactivity

Human, Mouse, Rat

• Applications

IF, IHC-P

• Application notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:200).

• Clonality

Monoclonal

• Isotype

Mouse IgG1

• Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

• Formulation

Liquid solution

• Concentration

1 mg/ml

• Storage buffer

PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

• Storage instructions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

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貨號	產品名稱	胞器染色位置	螢光波段
貨號KTC4003	TraKine™粒線體染色	粒線體(mitochondria)	Ex / Em = 490/523 nm	增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	微管蛋白(Tubulin)	Ex/Em = 500/520 nm	活細胞或固定之細胞
貨號KTC4001	Abbkine 細胞膜染色	細胞膜 (plasma membrane)	Ex/Em = 484/501 nm	活細胞、固定懸浮、貼附細胞
貨號BMD00063	DAPI Staining Solution	細胞核 dsDNA	Ex/Em: 358/461 nm (with DNA)	染色活細胞和固定細胞
貨號KTC4210	活細胞Lysosome染色	Lysosome	deep red fluorescent dye , Ex/Em:650/665nm	染色活細胞和固定細胞