COX-2抑制劑篩選試劑盒| COX-2 Inhibitor Screening Kit (Fluorometric) 貨號K547 (ab283401)
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COX-1抑制劑篩選K548 | COX活性測定試劑 | COX-2抑制劑篩選試劑 | 環氧合酶2（COX-2）ELISA試劑 | PTGS2 / COX-2（人類）ELISA試劑
COX-2抑制劑篩選試劑盒| 貨號K547 (ab283401)
COX-2 Inhibitor Screening Kit (Fluorometric)
環氧合酶（Cyclooxygenase , COX），也稱為前列腺素-內過氧化物合酶（prostaglandin-endoperoxide synthase , PTGS，EC 1.14.99.1），是一種酶，負責形成稱為前列腺素(prostanoids) 的重要生物介質，包括前列腺素(prostaglandins)，前列腺素(prostacyclin)和血栓烷(thromboxane)。 COX是從花生四烯酸(arachidonic acid)到前列腺素(prostanoids)的生物合成途徑中的中心酶。有兩種已知的同功酶(isoenzymes)：COX-1和COX-2。 COX-1在許多組織中組成性表達，並且是胃粘膜(gastric mucosa)和腎臟(kidney)中的主要形式。大多數細胞在正常條件下均不表達COX-2，但在炎症過程中發現其水平升高。非甾體抗炎藥（non-steroidal anti-inflammatory drugs, NSAID）對COX的藥理抑制作用可以緩解炎症和疼痛症狀。BioVision的COX-2抑制劑篩選試劑盒提供了一種快速，簡單，靈敏和可靠的測試方法，適用於高通量篩選COX-2抑制劑。該測定法基於對前列腺素G2（由COX酶產生的中間產物）的螢光檢測。
Inhibition of COX2 Activity with Celecoxib. IC50 of Celecoxib was determined to be 0.45 μM.
| Cat # +Size | K547-100 (ab283401) |
|---|---|
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em = 535/587 nm) |
| Applications | Screening/studying/characterizing COX-2 inhibitors. |
| Features & Benefits | • Simple method to screen COX-2 inhibitors • High-throughput • Includes Inhibitor Control, Celecoxib |
| Kit Components | • COX Assay Buffer • COX Probe (in DMSO) • COX Cofactor (in DMSO) • Arachidonic Acid • NaOH • COX-2, human recombinant • Celecoxib, COX-2 inhibitor (in DMSO) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
穀胱甘肽過氧化物酶活性測定 | FRAP檢測 | 總膠原蛋白分析 | Dopamine多巴胺檢測 | 三酸甘油酯分析 | Cholesterol Efflux膽固醇排出螢光分析 | 脂質過氧化（MDA）測定 | 支鏈氨基酸 BCAA檢測
| Cat # +Size | K547-100 |
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em = 535/587 nm) |
| Applications | Screening/studying/characterizing COX-2 inhibitors. |
| Features & Benefits | • Simple method to screen COX-2 inhibitors • High-throughput • Includes Inhibitor Control, Celecoxib |
| Kit Components | • COX Assay Buffer • COX Probe (in DMSO) • COX Cofactor (in DMSO) • Arachidonic Acid • NaOH • COX-2, human recombinant • Celecoxib, COX-2 inhibitor (in DMSO) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
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