BCAA支鏈氨基酸（Leu / Ile / Val）比色分析| Branched Chain Amino Acid Colorimetric Assay Kit貨號K564-100

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支鏈氨基酸（Leu / Ile / Val）比色分析試劑盒| 貨號K564-100

Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit

BCAA支鏈氨基酸 (branched-chain amino acids, BCAA) 或BCAA’s，是指具有非線性脂肪族側鏈的氨基酸 (non-linear aliphatic side-chains) ，即亮氨酸 (leucine)，異亮氨酸(isoleucine)和纈氨酸(valine)。BCAA中文為支鏈胺基酸（Branched-Chain Amino Acid）是人體中3種重要的必須胺基酸 (essential amino acids)，包括纈氨酸(valine)、亮氨酸( leucine)及異亮氨酸(isoleucine)（特徵為側鏈有分支結構）這三種必需氨基酸人體骨骼肌(skeletal muscle)的1/3左右組成。目前BCAA支鏈氨基酸被用於臨床上幫助燒傷受害者(burn victims)的康復(recovery)，以及為運動員 (athletes) 補充運動所需力量。 BCAA支鏈氨基酸，主要是Leu，可以刺激胰島素分泌(insulin)。支鏈氨基酸也牽涉( implicated) 到其他各種生理效應( physiological effects)。 BioVision的BCAA分析試劑盒提供了一種簡單方便的方法，來測量各種生物樣品中的BCAA。該試劑盒利用酵素測定BCAA氧化脫氨 (oxidatively deaminated)，產生NADH，減少探針，於450nm波長產生有色產物（λmax= 450nm）。 BioVision的BCAA試劑盒可測量每個樣品0到10 nmol範圍內的BCAA，檢測限為〜0.2 nmol（樣品中〜10μM BCAA）。支鏈氨基酸存在於每種約0.1-0.4mM的血清（〜0.125-1.5mM)

Cat # +Size	K564-100
Size	100 assays
Detection Method	Absorbance (450 nm)
Species Reactivity	Mammalian
Applications	BioVision’s BCAA kit measures BCAA’s in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample).
Features & Benefits	• Simple procedure; takes less than ~40 minutes • Fast and convenient
Kit Components	• BCAA Assay Buffer • BCAA Enzyme Mix • WST Substrate Mix • Leu Standard (1 µmol)

引用文獻

Chun-Fang Hsueh, The Effect of Branched-Chain Amino Acids, Citrulline, and Arginine on High-Intensity Interval Performance in Young Swimmers. Nutrients, Dec 2018; 30558143.

A. Wilson Aruni et al., VimA-Dependent Modulation of Acetyl Coenzyme A Levels and Lipid A Biosynthesis Can Alter Virulence in Porphyromonas gingivalis, Infect. Immun., Feb 2012; 80: 550 – 564.

Sample Preparation:

Tissue (20 mg) or cells (2 x 10⁶) can be homogenized with 100 µl Assay buffer. Centrifuge at 15,000g for 10 minutes to remove cell debris and other insoluble materials. Add samples to sample wells in a 96-well plate and bring the volume to 50 µl/well with Assay Buffer. We suggest testing several doses of your sample to make sure the readings are within the standard curve range. Typical volume for serum samples should be in the range of 1 – 20 µl.

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