O.D600菌液密度測量 | Biochrom WPA LED Cell Density meter CO8000 (貨號80-3000-45)
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Biochrom WPA CO8000攜帶式分光光度計 (貨號80-3000-45)
Biochrom WPA CO8000是一台LED攜帶式分光光度計；專用於測量懸浮之細胞密度，可應用於實驗室常規之菌液定量與細菌生長密度台之測量，如大腸桿菌(E.coli)、酵母(yeast)、細胞(cells) 和其他細胞培養物 (other cell cultures)。儀器簡單操作又可攜帶，手持式儀器具有一體式電池設計，可以攜帶至實驗預測量之位置，如孵化櫃、通風櫥或安全櫃中。
請注意，光束從前向後穿過cell chamber；確保單元插入正確對齊。
The Beer-Lambert Law and OD600
The Lambert-Beer-Law, also known as Beer’s Law, empirically relates the absorption of light to the properties of the sample. This law states that there is a logarithmic relationship between the transmission of light through a specific sample (T = I/Io with I = outgoing light and Io = incoming light), the molar extinction coefficient for a specific compound (ε), the concentration of the absorbing species in the material (c) and the distance the light travels (d).
OD estimations have gotten synonyms with estimations of bacterial concentration (C) or number (N), as per the Beer-Lambert law. However measurements of OD are actually measurements of turbidity, we can therefore apply the Beer-Lambert law, with certain contemplations, just for microbial colonies of low densities.
Biochrom WPA CO8000具有各種比色皿( cuvettes ) 和試管 (test tubes) ；包括10mm、12和16mm直徑的試管 (*需選配daptor set for 10 and 12mm tubes, 貨號80-3000-57 A)，可以與Erlenmeyer側臂燒瓶一起使用。儀器操作不需預熱時間，只需將其打開並準備好即可使用，與昂貴的分光光度計具相媲美的結果。可以測量培養物的細胞密度並將結果存儲在記憶中 。結果以吸光度單位（OD）呈現。完成所有測量後，可以在易於閱讀的屏幕上查看最多99個樣品結果，或者可以將儀器連接到PC或打印機並下載結果。(*需選配Spreadsheet interface software, 貨號80-2112-23。*使用Serial cable, 貨號80-3001-00 ) Optional accessories shown marked *Fixed with drain hole. Accepts 10mm pathlength semi micro and macro cuvettes or 14-17mm round tubes.
| Ordering Information | Order Code |
| Biochrom WPA CO8000 Cell Density Meter | 80-3000-45 |
| Spreadsheet interface software (requires serial cable) | 80-2112-23 |
| Serial cable | 80-3001-00 |
| Adaptor set for 10 and 12mm tubes | 80-3000-57 |
LED光源設計: 這種低維護儀器使用帶有光纖且無過濾器的無限壽命LED。當連接到主電源時，可充電電池會自動充電，電池充滿電大約12小時（過夜）一次充電可以正常使用1個月（在正常情況下）; 。它易於用乙醇( ethanol)清洗或用甲醛 (formaldehyde) 或環氧乙烷 (ethylene oxide0) 滅菌。
產品特點
- 單光束LED 細胞密度 (cell density meter) 於600nm測量
- 小巧便攜帶，可充電電池 ( rechargeable battery)
- LED光源壽命長 (Long lifetime) ，幾乎不需要維護
- 易於使用、清潔、消毒
- 最多可存儲99個結果，以便稍後下載
| Specification | Data |
|---|---|
| Type | Visible |
| 波長範圍 (Wavelength Range) | 600nm |
| 波長精準度 (Wavelength Accuracy (+/-)) | n/a |
| 波長再現性 ( Wavelength Reproducibility (+/-)) | n/a |
| 光源 (Lamp Source) | LED |
| 光學系統 (Beam) | Single Beam |
| 光源系統 (Optical System) | LED |
| 波寬 (Bandwidth) | 40nm |
| Detector | Diode |
| 測光範圍 (Photometric Range) | -0.3 to 1.99A |
| 測光線性度 (Photometric Accuracy) | +/- 0.02A at 1A |
| 測光再現性 (Photometric Reproducibility) | 0.02A at 1A in 10mm cuvette |
| 迷散光 (Stray Light) | <1%T |
| Measurement Type | Cell Density |
| Cell Compatibility | Cuvette 10mm |
| Other Sample Containers (選配) | Test tube * (10, 12, 16mm) |
| Cell Holder | Single Cell |
| Accessory Options | Rechargeable battery included |
| Method Storage | None |
| Compliance | CE |
| 資料輸出 (Data Output) (選配) | Print*, Store*, Export to PC* |
| Display | LCD |
| Data Interface | Serial |
| Languages | English, Manuals in French, German, Italian and Spanish |
| Power Requirement | 110-220V, 50/60Hz, 20VA |
| Weight | 0.6 Kg (1.2lb) |
| Dimensions (WxDxH) | 15 x 18 x 6cm (5.9 x 7.1 x 2.4 inches) |
Technical Specification shown below. Optional accessories shown marked *
WPA菌液密度測量儀器 | 安全核酸染劑商品 | Agarose | 微生物培養基 | 微生物抗生素 | 質體DNA萃取套組 | CMOS相機 |Mupid 2 DNA水平電泳槽| 桌上型滅菌釜
儀器操作方式 (簡易說明下戴)
光源由前至後, 請放置好Cuvette正確位置進行測量
1.按ON / OFF按鈕打開儀器。
2.將reference sample放入比色皿中。
3.按(Reference)鍵。螢幕顯示0.00。
4.取出reference sample，並用比色皿替換為樣品溶液。
5.按T(Measurement) 測量鍵。顯示屏將顯示樣品測量O.D (以吸光度單位表示)。
選擇memory鍵察看記錄; 若需清除記錄按二次reset鍵
The following table indicates the absolute minimum volume necessary for the correct function of the unit. The use of disposable plastic cuvettes is recommended.
下表列出了設備正常運作所需的絕對最小量。建議使用一次性塑膠比色皿(disposable plastic cuvettes)。
