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三酸甘油酯定量分析 | Triglyceride Quantification貨號K622-100 (Abcam ab65336)

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三酸甘油酯分析操作手冊

特點

三酸甘油酯呈色螢光分析套組Triglyceride Quantification Colorimetric/Fluorometric Kit (Abcam ab65336) 

三酸甘油酯呈色螢光分析套組 貨號K622-100 (100個分析)

Biovision提供一系列脂質分析套組;其中三酸甘油酯呈色螢光分析套組 (Triglyceride Quantification Kit, K622),一組產品可選擇呈色Absorbance (570 nm)或螢光Fluorescence (Ex/Em 535/587 nm) 兩種型態偵測Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm);可用於細胞培養液、尿液、血漿、或液體型式的來源。操作時間大約60分鐘。靈敏度2-10000µM (2 pmol-10nmol )。可測量甘油單酯(monoglycerides) 及甘油二酯( diglycerides)型式。甘油三酯(Triglyceride, TG)是植物油、animal fat動物脂肪,LDL密度脂蛋白和VLDL極低密度脂蛋白的主要成分,作為 transporters of fatty acids脂肪酸轉運體以及作為能源起著重要作用。 TG被分解成 脂肪酸(fatty acids)和甘油 (glycerol),然後兩者都可以作為 energy producing能量產生和 metabolic pathways.代謝途徑的substrates底物。 甘油三酯定量試劑 (Triglyceride Quantification Kit , K622) 盒提供了一種靈敏,簡便的測定方法,可用於測量各種樣品中的甘油三酯濃度。 在測定中,TG轉化為游離脂肪酸( free fatty acids) 和甘油(glycerol)。然後將甘油氧化以產生與探針反應產生顏色(λ= 570nm處的分光光度法)和螢光(Ex / Em = 535 / 583nm)的產物。 該試劑盒可以檢測各種樣品中2 pmol-10nmol(或2-10000μM範圍)的甘油三酯。

Triglyceride Quantification Colorimetric/Fluorometric Kit | BioVision Inc.

同質商品7

 

相關試劑 甘油三酯(TG)螢光檢測 | 前脂肪細胞分離試劑盒 | 甘油三酯(TG)螢光檢測-384well | 三酸甘油酯呈色/螢光分析 | 肝脂肪變性分析試劑盒

Cat # +Size K622-100 (Abcam ab65336)
Size 100 assays
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.
Features & Benefits • Simple procedure; takes ~1 hour
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of triglyceride in various biological samples
Kit Components • Triglyceride Assay Buffer
• TriglycerideProbe (in DMSO, anhydrous)
• Lipase
• TriglycerideEnzyme Mix (lyophilized)
• Triglyceride Standard (1 mM)

 

相關商品 乙醯乙酸呈色分析 | 3T3-L1分化試劑盒 | 脂肪形成呈色/螢光測定 | Adiponectin ELISA試劑盒 | 膽固醇排出螢光分析套組  | 長鏈游離脂肪酸分析套組 | 「Irisin」ELISA套組  | 三酸甘油酯呈色螢光分析

三酸甘油酯呈色螢光分析套組 | K622-100內容物

  • Triglyceride Assay Buffer
    • TriglycerideProbe (in DMSO, anhydrous)
    • Lipase
    • TriglycerideEnzyme Mix (lyophilized)
    • Triglyceride Standard (1 mM)
Benjamin S. Glicksberg, Integrative analysis of loss-of-function variants in clinical and genomic data reveals novel genes associated with cardiovascular traits. BMC Med Genomics, July 2019; 31345219.
Hui Cao, Functional role of SGK3 in PI3K/Pten driven liver tumor development. BMC Cancer, April 2019;  30975125.
Sarah H. Merkling, Peroxisome-associated Sgroppino links fat metabolism with survival after RNA virus infection in Drosophila. Sci Rep., Feb 2019;  30765784.
Qi Cao, Quantification of Hepatic Lipid Using 7.0T Proton Magnetic Resonance Spectroscopy and Computed Tomography in Mild Alcoholic Steatotic Mice. J Liver, Dec 2018;  30906674.
Emmelie Cansby, Targeted Delivery of Stk25 Antisense Oligonucleotides to Hepatocytes Protects Mice Against Nonalcoholic Fatty Liver Disease. Cell Mol Gastroenterol Hepatol, Dec 2018; 30576769.

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