環氧合酶2(COX-2)ELISA試劑盒| Human Cyclooxygenase-2 (COX-2) ELISA Kit貨號KTE61066

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COX-1抑制劑篩選K548 | COX活性測定試劑盒 | COX-2抑制劑篩選試劑盒 | 環氧合酶2(COX-2)ELISA試劑盒 | PTGS2 / COX-2(人類)ELISA試劑
環氧合酶2(COX-2)ELISA試劑盒| Human Cyclooxygenase-2 (COX-2) ELISA Kit貨號KTE61066
產品描述:環氧合酶2(COX-2)ELISA試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的人環氧合酶2(COX-2)。對人環氧合酶2(COX-2)特異的抗體已預先包被在微孔板上。將標準品和样品吸取到孔中,並且存在的任何纖維蛋白原都被固定的抗體結合。除去所有未結合的物質後,將HRP-人環氧合酶2(COX-2)檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的人環氧合酶2(COX-2)的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 1.25 ng/mL-20 ng/mL, 最低靈敏度0.1 ng/mL。可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum
- Product name
Human Cyclooxygenase-2 (COX-2) ELISA Kit
- Reactivity
Human
- Applications
ELISA
- Application notes
Human Cyclooxygenase-2 (COX-2) ELISA Kit employs a two-site sandwich ELISA to quantitate COX-2 in samples. An antibody specific for COX-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COX-2 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human COX-2 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of COX-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
- Detection method
Colorimetric
- Sample type
Cell culture supernatants, Other biological fluids, Plasma, Serum
- Assay type
Sandwich ELISA
- Calibration range
1.25 ng/mL-20 ng/mL
- Limit of detection
0.1 ng/mL
使用注意事項
- 請勿混用不同試劑盒中的組分,或在試劑盒到期日期之後使用試劑。
- 打開之前,讓所有試劑至少加室溫30分鐘。
- 用試劑預清洗移液器吸頭,為每個樣品,標準液和試劑使用新鮮的移液器吸頭,以免污染。
- 未使用的孔必須在提供的密封袋中於4°C乾燥。
- 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
- 建議所有樣品和標準品一式兩份或一式三份進行測定。
