人類丙烯醛 (ACR) ELISA 試劑盒 | Human Acrolein (ACR) ELISA Kit貨號 AE23507HU
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人類丙烯醛 (ACR) ELISA 試劑盒 | 貨號 AE23507HU
Human Acrolein (ACR) ELISA Kit
人類丙烯醛 (ACR) ELISA 試劑盒
Sandwich ELISA | 貨號AE23507HU。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Abebio 是一家領先的生物技術公司，專門生產高質量的生物產品，如蛋白質、抗體和 ELISA 試劑盒。Abebio 生產數以千計的 ELISA 試劑盒，用於檢測各種靶標，例如細胞因子(cytokines)、生長因子(growth factors)、趨化因子(chemokines)、激素(hormones)、病毒抗原(viral antigens)和重組蛋白(recombinant proteins)。
This assay employs a two-site sandwich ELISA to quantitate ACR in samples. An antibody specific for ACR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACR bound in the initial step. The color development is stopped and the intensity of the color is measured
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
| Species Reactivity | Human (Homo sapiens) |
| UniProt | |
| Abbreviation | ACR |
| Alternative Names | Preproacrosin|proacrosin |
| Range | Request Information |
| Sensitivity | Request Information |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Sandwich |
| Analysis Method | Quantitive |
| Assay Duration | 1-4.5h |
| Sample Volume | 1-200 μL |
| Detection Wavelengt | 450 nm |
人類丙烯醛 (ACR) ELISA 試劑盒 (酶聯免疫吸附試驗法) 套組內容
| Reagents | Quantity | Reagents | Quantity |
| Assay plate (96 Wells) | 1 | Instruction manual | 1 |
| Standard (lyophilized) | 2 | Sample Diluent | 1 x 20 mL |
| Biotin-Conjugate (concentrate 100 x) | 1 x 120 μL | Biotin-Conjugate Diluent | 1 x 20 mL |
| Streptavidin-HRP (concentrate 100 x) | 1 x 120 μL | Streptavidin-HRP Diluent | 1 x 20 mL |
| Wash Buffer (concentrate 25 x) | 1 x 20 mL | Substrate Solution | 1 x 12 mL |
| Stop Solution | 1 x 10 mL | Adhesive Films | 4 |
| Sample Type | Number | Recovery range (%) | Average(%) |
| Serum | 10 | 90-101 | 96 |
| EDTA plasma | 10 | 89-97 | 93 |
| Heparin plasma | 10 | 91-99 | 95 |
樣品採集和儲存
血清：使用血清分離管 (SST)，讓樣品在室溫下凝結 2 小時或在 2 – 8°C 下過夜，然後以 1000 × g 離心 15 分鐘。立即取出血清並進行檢測，或將樣品等分並儲存在 ≤ -20°C。避免重複凍融循環。血漿：使用 EDTA 或肝素作為抗凝劑收集血漿。收集後 30 分鐘內，在 2 – 8°C 下以 1000 × g 離心 15 分鐘。立即測定或分裝樣品並在 ≤ -20°C 下儲存。避免重複凍融循環。其他生物體液：以 1000 × g 離心樣品 20 分鐘。立即去除微粒並進行分析，或將樣品分裝在 -20°C 或 -80°C 下儲存。避免重複凍融循環。