豬經典豬瘟病毒抗體 (CSFV-Ab) ELISA 試劑盒 | 貨號AE64505PI
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豬經典豬瘟病毒抗體 (CSFV-Ab) ELISA 試劑盒 | 貨號AE64505PI
Pig Classical Swine Fever Virus Anitibody (CSFV-Ab) ELISA Kit
豬流行性腹瀉病毒（porcine epidemic diarrhea virus , PED 病毒或 PEDV）是一種冠狀病毒(coronavirus)，它會感染豬小腸(small intestine)內壁的細胞，導致豬流行性腹瀉(diarrhoea)，這是一種嚴重腹瀉(diarrhea)和脫水(dehydration)的情況。年長的豬大多在感染後生病並體重減輕(lose weight)，而新生仔豬(newborn piglets)通常在感染病毒後五天內死亡。 PEDV 不會傳播給人類，也不會污染人類的食物供應。由於 PEDV 具有高度傳染性，導致仔豬發病率(morbidity)和死亡率(mortality)顯著增加，因此造成了巨大的經濟負擔。接種疫苗的豬群的發病率和死亡率低於未接種疫苗的豬群，這表明出現了一種新的 PEDV 野毒株，目前基於 CV777 毒株的疫苗對這種毒株具有部分保護作用。消費者可能會以豬肉產品價格上漲的形式感受到病毒性疾病的影響。
豬經典豬瘟病毒抗體 (CSFV-Ab) ELISA 試劑盒 Competitive Inhibition |貨號AE64505PI。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Abebio 是一家領先的生物技術公司，專門生產高質量的生物產品，如蛋白質、抗體和 ELISA 試劑盒。 Abebio 生產數以千計的 ELISA 試劑盒，用於檢測各種靶標，例如細胞因子(cytokines)、生長因子(growth factors)、趨化因子(chemokines)、激素(hormones)、病毒抗原(viral antigens)和重組蛋白(recombinant proteins)。
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
| Species Reactivity | Pig (Sus scrofa; Porcine) | ||
| UniProt | N/A | ||
| Abbreviation | CSFV-Ab | ||
| Alternative Names | N/A | ||
| Range | Request Information | ||
| Sensitivity | Request Information | ||
| Sample Type | Serum, Plasma, Other biological fluids | ||
| Detection Method | Competitive ELISA | ||
| Analysis Method | Qualitative | ||
| Assay Duration | 1-3h | ||
| Sample Volume | 1-200 μL | ||
| Detection Wavelengt | 450 nm | ||
| Species Reactivity | Pig (Sus scrofa; Porcine) | ||
| UniProt | |||
| Abbreviation | Foot and Mouth Disease Virus O type Antibody | ||
| Alternative Names | N/A | ||
| Range | Request Information | ||
| Sensitivity | Request Information | ||
| Sample Type | Serum, Plasma, Other biological fluids | ||
| Detection Method | Competitive ELISA | ||
| Analysis Method | Qualitative | ||
| Assay Duration | 1-3h | ||
| Sample Volume | 1-200 μL | ||
| Detection Wavelengt | 450 nm | ||
This assay has high sensitivity and excellent specificity for detection of PEDV-Ab. No significant cross-reactivity or interference between PEDV-Ab and analogues was observed..
檢測試劑盒 (酶聯免疫吸附試驗法)套組內容
| Reagents | Quantity | Reagents | Quantity |
| Assay plate (96 Wells) | 1 | Instruction manual | 1 |
| Standard (lyophilized) | 2 | Sample Diluent | 1 x 20 mL |
| Biotin-Conjugate (concentrate 100 x) | 1 x 120 μL | Biotin-Conjugate Diluent | 1 x 20 mL |
| Streptavidin-HRP (concentrate 100 x) | 1 x 120 μL | Streptavidin-HRP Diluent | 1 x 20 mL |
| Wash Buffer (concentrate 25 x) | 1 x 20 mL | Substrate Solution | 1 x 12 mL |
| Stop Solution | 1 x 10 mL | Adhesive Films | 4 |
樣品採集和儲存
血清：使用血清分離管 (SST)，讓樣品在室溫下凝結 2 小時或在 2 – 8°C 下過夜，然後以 1000 × g 離心 15 分鐘。立即取出血清並進行檢測，或將樣品等分並儲存在 ≤ -20°C。避免重複凍融循環。血漿：使用 EDTA 或肝素作為抗凝劑收集血漿。收集後 30 分鐘內，在 2 – 8°C 下以 1000 × g 離心 15 分鐘。立即測定或分裝樣品並在 ≤ -20°C 下儲存。避免重複凍融循環。其他生物體液：以 1000 × g 離心樣品 20 分鐘。立即去除微粒並進行分析，或將樣品分裝在 -20°C 或 -80°C 下儲存。避免重複凍融循環。
