豬偽狂犬病gE病毒IgG抗體（PRV-gE-Ab-IgG）ELISA試劑盒 | 貨號AE64453PI
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豬偽狂犬病gE病毒IgG抗體（PRV-gE-Ab-IgG）ELISA試劑盒 | 貨號AE64453PI
Pig Pseudorabies gE virus IgG antibody (PRV-gE-Ab-IgG) ELISA Kit
豬偽狂犬病gE病毒IgG抗體（PRV-gE-Ab-IgG）ELISA試劑盒 Competitive Inhibition |貨號AE64453PI。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Abebio 是一家領先的生物技術公司，專門生產高質量的生物產品，如蛋白質、抗體和 ELISA 試劑盒。 Abebio 生產數以千計的 ELISA 試劑盒，用於檢測各種靶標，例如細胞因子(cytokines)、生長因子(growth factors)、趨化因子(chemokines)、激素(hormones)、病毒抗原(viral antigens)和重組蛋白(recombinant proteins)。
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
| Species Reactivity | Pig (Sus scrofa; Porcine) |
| UniProt | |
| Abbreviation | PRV-gE-Ab-IgG |
| Alternative Names | N/A |
| Range | Request Information |
| Sensitivity | Request Information |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Sandwich |
| Analysis Method | Quantitive |
| Assay Duration | 1-4.5h |
| Sample Volume | 1-200 μL |
| Detection Wavelengt | 450 nm |
This assay has high sensitivity and excellent specificity for detection of Pig PRV-Ab-IgG. No significant cross-reactivity or interference between Pig PRV-Ab-IgG and analogues was observed.
檢測試劑盒 (酶聯免疫吸附試驗法)套組內容
| Reagents | Quantity | Reagents | Quantity |
| Assay plate (96 Wells) | 1 | Instruction manual | 1 |
| Standard (lyophilized) | 2 | Sample Diluent | 1 x 20 mL |
| Biotin-Conjugate (concentrate 100 x) | 1 x 120 μL | Biotin-Conjugate Diluent | 1 x 20 mL |
| Streptavidin-HRP (concentrate 100 x) | 1 x 120 μL | Streptavidin-HRP Diluent | 1 x 20 mL |
| Wash Buffer (concentrate 25 x) | 1 x 20 mL | Substrate Solution | 1 x 12 mL |
| Stop Solution | 1 x 10 mL | Adhesive Films | 4 |
Matrices listed below were spiked with certain level of recombinant Pig PRV-gE-Ab-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Pig PRV-gE-Ab-IgG in samples.
| Sample Type | Number | Recovery range (%) | Average(%) |
| Serum | 10 | 90-101 | 96 |
| EDTA plasma | 10 | 89-97 | 93 |
| Heparin plasma | 10 | 91-99 | 95 |
樣品採集和儲存
血清：使用血清分離管 (SST)，讓樣品在室溫下凝結 2 小時或在 2 – 8°C 下過夜，然後以 1000 × g 離心 15 分鐘。立即取出血清並進行檢測，或將樣品等分並儲存在 ≤ -20°C。避免重複凍融循環。血漿：使用 EDTA 或肝素作為抗凝劑收集血漿。收集後 30 分鐘內，在 2 – 8°C 下以 1000 × g 離心 15 分鐘。立即測定或分裝樣品並在 ≤ -20°C 下儲存。避免重複凍融循環。其他生物體液：以 1000 × g 離心樣品 20 分鐘。立即去除微粒並進行分析，或將樣品分裝在 -20°C 或 -80°C 下儲存。避免重複凍融循環。
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pig PRV-gE-Ab-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample Type | 1：2 | 1：4 | 1：8 | 1：16 |
| Serum | 78-89% | 81-99% | 92-103% | 95-105% |
| EDTA plasma | 91-101% | 90-98% | 93-101% | 91-98% |
| Heparin plasma | 92-103% | 93-102% | 92-99% | 91-101% |