豬經典豬瘟病毒抗體 (CSFV-Ab) ELISA 試劑盒 | 貨號AE64456PI
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豬經典豬瘟病毒抗體 (CSFV-Ab) ELISA 試劑盒 | 貨號AE64456PI
Pig Classical Swine Fever Virus Anitibody (CSFV-Ab) ELISA Kit
經典豬瘟(lassical swine fever, CSF)是一種豬的急性、強烈的傳染性疾病，對養豬業造成重大損害。腦脊液可引起豬全身器官和組織出現彌散性出血，小血管(small vascular)和毛細血管內皮細胞(capillary endothelial cells)變性壞死(necrosis)。豬瘟病毒（CSFV）屬於瘟病毒屬黃病毒科(Pestivirus, family Flaviviridae)，基因組全長12.3Kb，包括一個大的ORF，編碼11個結構和非結構蛋白(nonstructural proteins)。
豬經典豬瘟病毒抗體 (CSFV-Ab) ELISA 試劑盒 Competitive Inhibition|貨號AE64456PI。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Abebio 是一家領先的生物技術公司，專門生產高質量的生物產品，如蛋白質、抗體和 ELISA 試劑盒。 Abebio 生產數以千計的 ELISA 試劑盒，用於檢測各種靶標，例如細胞因子(cytokines)、生長因子(growth factors)、趨化因子(chemokines)、激素(hormones)、病毒抗原(viral antigens)和重組蛋白(recombinant proteins)。
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
| Species Reactivity | Pig (Sus scrofa; Porcine) |
| UniProt | N/A |
| Abbreviation | CSFV-Ab |
| Alternative Names | N/A |
| Range | Request Information |
| Sensitivity | Request Information |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Competitive ELISA |
| Analysis Method | Qualitative |
| Assay Duration | 1-3h |
| Sample Volume | 1-200 μL |
| Detection Wavelengt | 450 nm |
檢測試劑盒 (酶聯免疫吸附試驗法)套組內容
| Reagents | Quantity | Reagents | Quantity |
| Assay plate (96 Wells) | 1 | Instruction manual | 1 |
| Standard (lyophilized) | 2 | Sample Diluent | 1 x 20 mL |
| Biotin-Conjugate (concentrate 100 x) | 1 x 120 μL | Biotin-Conjugate Diluent | 1 x 20 mL |
| Streptavidin-HRP (concentrate 100 x) | 1 x 120 μL | Streptavidin-HRP Diluent | 1 x 20 mL |
| Wash Buffer (concentrate 25 x) | 1 x 20 mL | Substrate Solution | 1 x 12 mL |
| Stop Solution | 1 x 10 mL | Adhesive Films | 4 |
Recovery
Matrices listed below were spiked with certain level of recombinant Classical SwineFever Virus Anitibody and the recovery rates were calculated by comparing the measured value to the expected amount of Classical SwineFever Virus Anitibody in samples.
| Sample Type | Number | Recovery range (%) | Average(%) |
| Serum | 10 | 90-101 | 96 |
| EDTA plasma | 10 | 89-97 | 93 |
| Heparin plasma | 10 | 91-99 | 95 |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Classical SwineFever Virus Anitibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample Type | 1：2 | 1：4 | 1：8 | 1：16 |
| Serum | 78-89% | 81-99% | 92-103% | 95-105% |
| EDTA plasma | 91-101% | 90-98% | 93-101% | 91-98% |
| Heparin plasma | 92-103% | 93-102% | 92-99% | 91-101% |
樣品採集和儲存
血清：使用血清分離管 (SST)，讓樣品在室溫下凝結 2 小時或在 2 – 8°C 下過夜，然後以 1000 × g 離心 15 分鐘。立即取出血清並進行檢測，或將樣品等分並儲存在 ≤ -20°C。避免重複凍融循環。血漿：使用 EDTA 或肝素作為抗凝劑收集血漿。收集後 30 分鐘內，在 2 – 8°C 下以 1000 × g 離心 15 分鐘。立即測定或分裝樣品並在 ≤ -20°C 下儲存。避免重複凍融循環。其他生物體液：以 1000 × g 離心樣品 20 分鐘。立即去除微粒並進行分析，或將樣品分裝在 -20°C 或 -80°C 下儲存。避免重複凍融循環。
