Anti-GAPDH單株抗體| Anti-GAPDH Mouse Monoclonal Antibody (2B5) Abbkine貨號ABL1020
| 代理廠牌： |  |
| 原廠連結： | |
| 相關下載： |
Anti-GAPDH單株抗體| Anti-GAPDH Mouse Monoclonal Antibody (2B5) Abbkine貨號ABL1020
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
GAPDH單株抗體可用做loading control antibody。GAPDH (甘油醛 3-磷酸脫氫酶, Glyceraldehyde 3-phosphate dehydrogenase, 縮寫為 GAPDH 或 G3PDH）是一種 37kDa 的酶，可催化糖酵解的第六步，從而分解葡萄糖(glucose)以獲取能量(energy)和碳分子(carbon molecules)。除了這種長期建立的代謝功能(metabolic function)外，GAPDH 最近還參與了幾種非代謝過程(non-metabolic processes)，包括轉錄激活( transcription activation)、細胞凋亡 ER 到高爾基體小泡穿梭(Golgi vesicle shuttling)的啟動，以及快速軸突(fast axonal)或軸漿運輸(axoplasmic transport)。
產品介紹: GAPDH單株抗體，免疫原Synthetic Peptide。可應用於IF, IHC-P, WB實驗。反應物種 (Chicken, Dog, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Yeast)；最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting dilutions are as follows: WB(1:10000-1:100000), IHC-P (1:400), IF (1:400)。使用特異性免疫原通過親和層析從小鼠腹水中親和純化抗體。保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol。
Fig.1. Western blot analysis of Hela (1), rat brain (2), rabbit muscle(3), sheep muscle(4), and mouse brain (5), diluted at 1:10000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Fig.3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Fig.5. Immunofluorescence analysis of human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.6. Immunofluorescence analysis of mouse liver tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.7 Western blot analysis of Pig muscle tissue, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).
| Product name | Anti-GAPDH Mouse Monoclonal Antibody (2B5) |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Reactivity | Chicken, Dog, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Yeast |
| Applications | IF, IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB(1:10000-1:100000), IHC-P (1:400), IF (1:400). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. |
| Gene ID | 2597 |
| Alternative | GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH |
| Accession | P04406 |
| Observed Band(KD) | 37 |
預染protein marker | 快速型蛋白質染色劑 | 活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 | Abbkine 細胞膜染色 | 活細胞Lysosome染色
Abbkine提供一系列高度特異性單克隆和多克隆抗體，tag antibody抗體系列商品如 His tag、GST tag、S tag、HA tag、DDDDK tag、Myc tag、MBP tag和 mCherry tag等單/多株Tag抗體。另具生物素(Biotin)、HRP、AbFluor™ 染料、瓊脂糖(agarose)和磁珠偶聯物(magnetic beads)標誌。
| Cat.No | Name | Size1 |
| KTD104-EN | Universal IP/Co-IP Toolkit (Magnetic Beads) | 20T |
| KTD105-EN | Universal IP/Co-IP Toolkit (Agarose) | 20T |
| A25012 | IPKine™ HRP, Goat Anti-Mouse IgG LCS | 100 μL |
| A25022 | IPKine™ HRP, Mouse Anti-Rabbit IgG LCS | 100 μL |
| A25112 | IPKine™ HRP, Goat Anti-Mouse IgG HCS | 100 μL |
| A25122 | IPKine™ HRP, Mouse Anti-Rabbit IgG HCS | 100 μL |
| A25222 | IPKine™ HRP, Goat Anti-Rabbit IgG HCS | 100 μL |
| KTI2014 | IPKine™ Anti-DDDDK Magnetic IP Kit | 20T |
| KTI2024 | IPKine™ Anti-GFP Magnetic IP Kit | 20T |
| KTI2034 | IPKine™ Anti-GST Magnetic IP Kit | 20T |
| KTI2044 | IPKine™ Anti-HA Magnetic IP Kit | 20T |
| KTI2064 | IPKine™ Anti-Myc Magnetic IP Kit | 20T |
| ABT2013 | Agarose Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 1 mL |
| ABT2014 | Magnetic Beads Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 1 mL |
| ABT2024 | Magnetic Beads Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 1 mL |
| ABT2023 | Agarose Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 1 mL |
| ABT2043 | Agarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 1 mL |
| ABT2044 | Magnetic Beads Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 1 mL |
| ABT2053 | Agarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 1 mL |
| ABT2054 | Magnetic Beads Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 1 mL |
| ABT2063 | Agarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 1 mL |
| ABT2064 | Magnetic Beads Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 1 mL |
| ABT2173 | Agarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 1 mL |
| ABT2174 | Magnetic Beads Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 1 mL |
| ABT2010 | Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 50 μL |
| ABT2011 | Anti-DDDDK Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2015 | HRP Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 50 μL |
| ABT2020 | Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 50 μL |
| ABT2025 | HRP Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 50 μL |
| ABT2021 | Anti-GFP Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2030 | Anti-GST Tag Mouse Monoclonal Antibody (2A8) | 50 μL |
| ABT2035 | HRP Conjugated Anti-GST Tag Mouse Monoclonal Antibody (2A8) | 50 μL |
| ABT2040 | Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 50 μL |
| ABT2041 | Anti-HA Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2045 | HRP Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 50 μL |
| ABT2050 | Anti-His Tag Mouse Monoclonal Antibody (5C3) | 50 μL |
| ABT2051 | Anti-His Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2055 | HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 50 μL |
| ABT2060 | Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 50 μL |
| ABT2061 | Anti-Myc Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2065 | HRP Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 50 μL |
| ABT2170 | Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 50 μL |
| ABT2070 | Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) | 50 μL |
| ABT2080 | Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) | 50 μL |
| ABT2090 | Anti-E2 Tag Mouse Monoclonal Antibody (12T4) | 50 μL |
| ABT2100 | Anti-HSV Tag Mouse Monoclonal Antibody (16T2) | 50 μL |
| ABT2110 | Anti-KT3 Tag Mouse Monoclonal Antibody (14D8) | 50 μL |
| ABT2120 | Anti-RFP Tag Mouse Monoclonal Antibody (9D1) | 50 μL |
| ABT2130 | Anti-S Tag Mouse Monoclonal Antibody (9T10) | 50 μL |
| ABT2150 | Anti-T7 Tag Mouse Monoclonal Antibody (6D4) | 50 μL |
| ABT2160 | Anti-Trx Tag Mouse Monoclonal Antibody (14D4) | 50 μL |
| ABT2180 | Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2) | 50 μL |
| ABT2190 | Anti-CBP Tag Monoclonal Antibody (12H5) | 50 μL |
| ABT2200 | Anti-TAP Tag Monoclonal Antibody (4H2) | 50 μL |
| ABT2210 | Anti-Avi-Tag Monoclonal Antibody (5G11) | 50 μL |
| ABT2220 | Anti-SRT-Tag Monoclonal Antibody (11G3) | 50 μL |
| ABT2230 | Anti-Strep-Tag II Monoclonal Antibody (8C12) | 50 μL |
| ABT2240 | Anti-mStrawberry Monoclonal Antibody (4C9) | 50 μL |
| ABT2250 | Anti-EYFP-Tag Monoclonal Antibody (10T3) | 50 μL |
| ABT2260 | Anti-mOrange Monoclonal Antibody (9A10) | 50 μL |
| ABT2270 | Anti-AmCyan Monoclonal Antibody (8T2) | 50 μL |
| ABT2280 | Anti-ECFP-Tag Monoclonal Antibody (6B11) | 50 μL |
| ABT2290 | Anti-EBFP Monoclonal Antibody (8B5) | 50 μL |
| ABT2300 | Anti-Nano-Tag9 Monoclonal Antibody (11T3) | 50 μL |