瓜氨酸(Citrulline)分析試劑盒 | Citrulline Fluorometric Assay Kit 貨號K2002-100

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原廠連結：	https://www.biovision.com
相關下載：	瓜氨酸(Citrulline)分析試劑盒操作手冊

特點

瓜氨酸化(Citrullination)是肽精氨酸精氨酸去亞氨酶（peptidylarginine deiminases , PAD）將氨基酸精氨酸(amino acid arginine)轉化為靶蛋白中的瓜氨酸(Citrulline)。瓜氨酸(Citrulline)不是20種標準氨基酸之一，而是翻譯後修飾(post-translational modification)的結果。蛋白質的瓜氨酸化對於維持正常的生理功能非常重要，包括通過組蛋白修飾(histone modification)，免疫反應(immune responses)等調節基因。然而，瓜氨酸化(Citrullination)度增加與免疫病理狀況和神經系統疾病, 如類風濕性關節炎(rheumatoid arthritis)，多發性硬化症(multiple sclerosis)和阿爾茨海默氏病(Alzheimer’s disease）有關。 BioVision自豪地提供全面的檢測試劑盒，蛋白質和酶，抗體和生化試劑組合，以促進瓜氨酸化研究(citrullination research)。

for measuring total Citrulline concentrations

產品介紹: L-瓜氨酸(L-Citrulline)是一種非蛋白半必需氨基酸(non-proteogenic, semi-essential amino acid)。瓜氨酸(Citrulline)通過鳥氨酸氨基甲基轉移酶(ornithine carbamoyltransferase)或作為一氧化氮合酶（Nitric oxide synthase ,NOS）活性的副產物形成。 NOS反應中產生的瓜氨酸(Citrulline)可通過尿素循環中的兩種酶精氨琥珀酸合成酶（argininosuccinate synthetase , ASS）和精氨琥珀酸裂合酶（argininosuccinate lyase , ASL）再循環為精氨酸。已證明在具有ASS或ASL基因突變的患者中瓜氨酸(Citrulline)濃度升高。西瓜(Watermelon)是瓜氨酸(Citrulline)的最豐富來源之一。此外，由於瓜氨酸(Citrulline)參與一氧化氮（NO）的合成，有助於血管舒張，因此已被宣傳為運動營養補充劑(sports nutrition supplement)。 BioVision的瓜氨酸(Citrulline)分析試劑盒提供了一種快速，特異性和簡便的方法來測量各種樣品中瓜氨酸的總濃度。在這種酶促測定中，瓜氨酸轉化為一系列中間體，進一步與探針反應產生穩定的螢光信號（Ex / Em = 535/587 nm）。該套件簡單，易於執行，靈敏並且具有高通量適應性。它可以檢測出生物樣品中低至2 µM的瓜氨酸。

Cat # +Size	K2002-100
Size	100 assays
Kit Summary	L-Citrulline is a non-proteogenic, semi-essential amino acid. Citrulline is formed either by ornithine carbamoyltransferase or as a byproduct of the Nitric oxide synthase (NOS) activity. Citrulline generated in the NOS reaction can be recycled to arginine by the two enzymes argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL) acting in the Urea Cycle. Citrulline concentrations have been shown to be elevated in patients with mutated ASS or ASL genes. Watermelon is one of the richest sources of Citrulline. Additionally, Citrulline has been advertised as sports nutrition supplement due to its involvement in Nitric Oxide (NO) synthesis, which helps with vasodilation. BioVision’s Citrulline Assay Kit provides a rapid, specific, and easy method for the measurement of total Citrulline concentrations in a wide variety of samples. In this enzymatic assay, Citrulline is converted into a series of intermediates, which further reacts with a probe producing a stable fluorescent signal (Ex/Em = 535/587 nm). The kit is simple, easy to perform, sensitive and is highthroughput adaptable. It can detect as low as 2 µM Citrulline in biological samples.
Detection Method	Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity	All
Applications	• Analysis of Urea cycle and NO cycle • Measurement of Citrulline in beverages and biological samples.
Features & Benefits	• Easy method for the measurement of total Citrulline concentrations in a wide variety of samples. • Specific • Rapid
Kit Components	Citrulline Developer Mix Citrulline Enzyme Mix Citrulline Cofactor Mix Citrulline Converter Mix Citrulline Buffer Supplement Citrulline Assay Buffer Citrulline Standard Citrulline Probe
Storage Conditions	-20°C
Shipping Conditions	Gel Pack
USAGE	For Research Use Only! Not For Use in Humans.


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