Human CellExp™ PDGFRb|CD140b人類重組蛋白 貨號7493-50

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Human CellExp™ PDGFRb/CD140b人類重組蛋白 貨號7493-50
Human CellExp™ PDGFRb/CD140b

產品介紹: 人血小板衍生的生長因子受體β多肽(Human platelet-derived growth factor receptor , PDGFRB),也稱為J03278,M21616,CD140B,JTK12,PDGF-R-beta和PDGFR,是與PDGFB特異性結合併具有酪氨酸蛋白激酶活性(tyrosine-protein kinase activity的)受體。血小板衍生生長因子(PDGF)家族成員的細胞表面酪氨酸激酶受體PDGFR / PDGF系統包括兩個受體(PDGFRA和PDGFRB)和四個配體(A,B,C和D),受體PDGFRA和PDGFRB它們在序列上相關,並且都是受體酪氨酸激酶(RTK)的III類亞型的成員;其他III類RTK是CSF1R,KIT和FLT3; PDGF的結合誘導受體同型和異二聚化以及信號轉導。 β受體在各種細胞類型中受到獨立調節,可溶性可溶性PDGFRB以高親和力結合PDGF,是有效的PDGF拮抗劑,這些配體形成同二聚體或異二聚體(PDGF-AA,-AB,-BB,-CC,-DD)。四個PDGF在其monomeric forms處於非活動狀態PDGF與蛋白酪氨酸激酶受體PDGF受體α和-β結合。這兩種受體同工型在結合PDGF二聚體時會二聚化,從而導致三種可能的受體組合,即-αα,-ββ和-αβ.PDGF- CC專門與PDGFR-αα和-αβ相互作用,但不與–ββ相互作用,因此類似於PDGF-AB,PDGF-DD以高親和力與PDGFR-ββ結合,而與PDGFR-αβ的結合程度較低,被認為是PDGFR -ββ特異性PDGF-AA僅與PDGFR-αα結合,而PDGF-BB是唯一可以高親和力結合所有三種受體組合的PDGF。
| Alternate Name | PDGFRB, PDGFR-B, CD140B, CD-140B, JTK12, JTK-12, PDGF-R-beta, PDGFR, PDGFR1, PDGFR-1, PDGFR-beta, PDGFR-β, PDGF-R-β |
| Gene Symbol | PDGFRB |
| Gene ID | 5159 |
| Accession # | P09619 |
| Source | HEK293 cells |
| Appearance | Lyophilized |
| Physical Form Description | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
| Molecular Weight | This protein contains C-terminal polyhistidine tag and has a calculated MW of 57.1 kDa. As a result of glycosylation, DTT-reduced protein migrates as 85-95 kDa polypeptide in SDS-PAGE. |
| Purity by SDS-PAGE | ≥95% |
| Endotoxin Level | <1 EU/μg by LAL method |
| Biological Activity | Measured by its ability to inhibit the biological activity of PDGFBB using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 3-8 μg/ml in the presence of 4 ng/ml rhPDGFBB. |
| Reconstitution Instructions | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
穀胱甘肽過氧化物酶活性測定 | FRAP檢測 | 總膠原蛋白分析 | Dopamine多巴胺檢測 | 三酸甘油酯分析 | Cholesterol Efflux膽固醇排出螢光分析 | 脂質過氧化(MDA)測定 | 支鏈氨基酸 BCAA檢測
| 貨號9210 | 重組蛋白酶K (分子等級RNase/DNase free) | 100 mg, 500 mg, 1 g, 10 g, 50 g | 純度 99% (Native PAGE and SDS PAGE assay) |
| 貨號9211-5 | 重組蛋白酶K (分子等級RNase/DNase free) | 5 ml, 25 ml, 100 m (20 mg/ml Solution) | 純度 99% (Native PAGE and SDS PAGE assay) |
| 貨號9247 | 重組蛋白酶K (NGS等級) | 100 mg, 500 mg, 1 g, 10 g |
重組酶在Pichia pastoris中表達, 並進行廣泛的純化以獲得最高的 優質的產品。額外的純化步驟導致 溶解度顯著增加(2.5倍),增加 比活性和減少的DNA含量
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| 貨號9250 | 重組蛋白酶K (標準等級) | 5 ml, 25 ml, 100 m (20 mg/ml Solution) | 純度>90% |
| 貨號9251 | 重組蛋白酶K (標準等級) | 100 mg, 500 mg ,1 g ,10 g | 純度>90% |
