AbFluor™ 488-Phalloidin F-actin肌動蛋白染色| Ex/Em = 490/515 nm貨號BMD0082
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AbFluor™ 488-Phalloidin (Ex/Em = 490/515 nm)
F-actin肌動蛋白染色套組 (AbFluor™ 488-Phalloidin) | 貨號BMD0082
F-actin肌動蛋白是一種大約42 kDa的蛋白質，幾乎在所有真核細胞中都有發現，它也是最高度保守的蛋白質之一。肌動蛋白的單體為球形分子，稱為球形肌動蛋白G-actin，它的多聚體稱為纖維形肌動蛋白F-actin。這兩者對於細胞分裂過程中細胞的移動和收縮等重要的細胞功能都是必不可少的。AbFluor™ 488-Phalloidin選擇性地結合F-actins, 它比氟西林-鄰苯二甲a)的抗性要高得多。Phalloidin衍生物 是標記、識別和定量甲醛固定(formaldehyde-fixed )和滲透組織部分( permeabilized tissue sections)中的F-actin的方便探針,可在細胞培養物中染色F-actin或cell-free experiments at nanomolar levels。
鄰苯二甲苯甲類毒素(Phalloidin )
Phalloidin 為一種帶有毒性的胜肽，具有與 F-actin 專一性結合的特性。屬於一類稱為phallo毒素(phallotoxins)的毒素,這些毒素與死亡帽蘑菇(阿馬尼塔phalloides)分離。它是一種二環肽(bicyclic peptide ),特別與F-actin結合。因此,使用螢光染料標記的鄰苯二甲二素素,可以非常方便地研究F-actin的分佈。在噬菌素內部,半胱氨酸和色氨酸之間有一個不尋常的硫醚橋,可以形成一個內環結構。當pH升高時,硫醚被切合,鄰苯二甲酸酯失去對作用素的親和力。標定有螢光染料的 Phalloidin 試劑產品，能夠協助研究人員精確掌握細胞內 F-actin 的分布情形、了解 F-actin 表現量變化。
產品介紹: AbFluor™ F-actin 488-Phalloidin染色試劑盒(Gree Fluorescence, 綠色螢光)使用綠色螢光(Ex/Em = 490/515 nm)鄰苯二甲素偶聯,選擇性地結合F-actins,其光穩定性遠遠高於其他氟素-噬菌素偶聯(fluorescein-phalloidin conjugates)。Phalloidin衍生物(fluorescein-phalloidin conjugates.)是標記、識別和定量甲醛固定和滲透組織部分中的F-actin探針,可在細胞培養物中染色F-actin或cell-free experiments at nanomolar levels。
Phalloidin分子式為C35H48N8O11S，分子量788.87，CAS#17466-45-4。Phalloidin屬於一類稱為鬼筆毒素(phallotoxins)的毒素，它是從死帽蘑菇 (Amanita phalloides) 中分離出來的。它是一種雙環肽(Amanita phalloides)，可特異性結合 F-肌動蛋白(F-actin )。因此，使用螢光染料標記的鬼筆環肽可以非常方便地研究 F-肌動蛋白(F-actin )的分佈。在鬼筆環肽內部，半胱氨酸和色氨酸之間有一個不尋常的硫醚橋(thioether bridge )，可以形成內環結構( inner ring structure)。當 pH 升高時，硫醚(thioether )被裂解，phalloidin失去對肌動蛋白的親和力。 AbFluor™ 594-phalloidin選擇性結合 F-肌動蛋白，它的光穩定性比fluorescein-phalloidin conjugates高得多。Phalloidin derivatives衍生物是方便的探針，用於標記、鑑定和定量甲醛固定(formaldehyde-fixed )和透化組織切片(permeabilized tissue sections)中的 F-肌動蛋白，可在納摩爾水平(nanomolar levels)對細胞培養或無細胞實驗中的 F-肌動蛋白進行染色。
- FormulationLyophilized powder
- Features & Benefits
- Optimized staining protocol for labeling, identifying and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
- Proprietary AbFluor™ 488-Phalloidin (Green Fluorescence) (Ex/Em = 490/515 nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.
- Usage notesPhalloidin is toxic, it should be handled with care.
- Storage instructionsStable for at least 12 months if store at -20°C. Protect this product from light.
- ShippingGel pack with blue ice.
| 產品貨號 | 產品名稱 | 規格 |
| 貨號BMD0082 | F-actin肌動蛋白染色套組 (AbFluor™ 488-Phalloidin) |
500個反應 綠色螢光(Ex/Em = 490/515 nm) |
| 貨號BMD00083 | F-actin肌動蛋白染色套組 (AbFluor™ 555-Phalloidin) |
500個反應 橙色螢光(Ex/Em = 555/565 nm) |
| 貨號BMD00084 | F-actin肌動蛋白染色套組 (AbFluor™ 594-Phalloidin) |
500個反應 紅色螢光 (Red Fluorescence) (Ex/Em = 593/614 nm) |
| 貨號KTC4008 | TraKine™ F-actin肌動蛋白染色套組 (green fluorescent ) |
300個反應 綠色螢光(Ex/Em = 490/515 nm) |
| 貨號KTC4008 | TraKine™ F-actin肌動蛋白染色套組 (green fluorescent ) |
1000個反應 綠色螢光(Ex/Em = 490/515 nm) |
| 貨號KTC4009 | TraKine™F-actin肌動蛋白染色套組 |
300個反應 橙色螢光(Ex/Em = 593/614nm) |
| 貨號KTC4009 | TraKine™F-actin肌動蛋白染色套組 |
1000個反應 橙色螢光(Ex/Em = 593/614nm) |
部分實驗結果圖片： 下圖為使用AbFluor™ 488標記的鬼筆環肽對A594細胞進行染色，藍色為DAPI染核
| 貨號 | 產品名稱 | 胞器染色位置 | 螢光波段 | |
| 貨號KTC4003 | 粒線體染色 | 粒線體(mitochondria) | Ex / Em = 490/523 nm | 增殖(proliferating)和非增殖細胞(non-proliferating cells) |
| 貨號KTC4100 | TraKine™ Pro*活細胞微管蛋白染色 | 微管蛋白(Tubulin) | Ex/Em = 500/520 nm | 活細胞或固定之細胞 |
| 貨號KTC4001 | 細胞膜染色 | 細胞膜 (plasma membrane) | Ex/Em = 484/501 nm | 活細胞、固定懸浮、貼附細胞 |
| 貨號KTC4210 | TraKine™ Pro* 活細胞Lysosome染色 | 溶酶體Lysosome | deep red fluorescent dye , Ex/Em:650/665nm | 染色活細胞和固定細胞 |
| 貨號BMD00063 | DAPI Staining Solution | 細胞核 dsDNA | Ex/Em: 358/461 nm (with DNA) | 染色活細胞和固定細胞 |
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