逆轉錄酶 RScript Reverse Transcriptase (250 Reactions) | Bio-Helix 貨號RT001-0250
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逆轉錄酶RScript Reverse Transcriptase / 250 Reactions| Bio-Helix 貨號RT001-0250
RScript Reverse Transcriptase / 250 Reactions
產品描述:
Overcome the most challenging RNA structures over a wide temperature range.
Bio-Helix RScript 逆轉錄酶(Bio-Helix RScript Reverse Transcriptase) – 專為研究和診斷應用而創新設計，滿足所有的 cDNA 合成需求，並克服寬溫度範圍內最具挑戰性的二級 RNA 結構( secondary RNA structures)。新一代工程重組 M-MLV 逆轉錄酶( M-MLV reverse transcriptase)具有更高的熱穩定性(thermostability)、持續合成能力(processivity)、穩健性(robustness)、最佳 cDNA 產量、用於降低 RNase H 活性的專有位點突變以及延長的半衰期，是世界上最通用的逆轉錄酶( versatile reverse transcriptase )不僅簡單地滿足常規 cDNA 合成要求，而且即使是手頭最具挑戰性的 RNA 樣品也能實現卓越的性能。
Figure 1. Stability of RScript reverse transcriptase:
The stability RT-qPCR data shows the performance of RScript reverse transcriptase is maintained without any significant alteration in performance at 37 °C after a period of 3 weeks (21 days), demonstrating its high resistance to temperature and time. The percentage of RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.
A: Bio-Helix RScript reverse transcriptase #RT001-0050/ RT001-0250
B: Promega Improm II #A3802
C: Thermo SuperScript III #18080093
D: SuperScript IV #18090010
Figure 2. Enhancement of the thermostability:
Reverse transcription of a 1 kb fragment ARHGAP29 RNA using RScript reverse transcriptase or competitor reverse transcriptases were used to carry this experiment. Regardless of the variations in temperatures (42 -60 °C) RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 ℃ compared to other brands. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R.
Figure 3. Elongation of RNA templates up to 11 kb in length :
RScript reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines – 5637 (HTB-9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R.
Figure 4. Performance of One-Step RT-qPCR with RScript reverse transcriptase :
To evaluate the RT performance for the COVID-19 fragments, the N1 gene with 100 copies per reaction was tested in the one-step RT-qPCR. Experiments proved to be feasible and effective (mean Ct : 34.88).
* COVID-19 fragments were originally obtained from Twist Synthetic SARS-CoV-2 RNA Controls (# EPI_ISL_1662307).
Red: Bio-HeliX RScript reverse transcriptase #RT001-0050
Green: Improm-II #A3802
Blue: Superscript IV #18090010
Figure 5. Performing RT-qPCR with RScript reverse transcriptase :
At a temperature of 55 °C, RScript reverse transcriptase exceeds performance demonstrating better efficiency and higher cDNA yields. RScript reverse transcriptase shows a stable and superior performance at 55 ℃ compared to other brands. Therefore RScript reverse transcriptase does not encounter significant higher Ct values compared to Improm II and SuperScript IV due to their low amounts of input cDNA.
*Promega Improm-II #A3802 is a registered trademark of Promega, Thermo Superscript-III #18080093 and Superscript-IV #18090010 are registered trademarks of Thermo Scientific. The trademark holders are not affiliated with Bio-Helix Co., Ltd. and do not endorse these products.
產品特徵:
- Excellent reverse transcriptase for challenging COVID-19 RNA detection.
- Enzyme activity stable even when stored at 37°C over 21 days.
- Enhanced thermostability from 42°C to 60°C.
| RK001-0050 cDNA合成KIT |
RScript™ cDNA Synthesis Kit (新一代工程重組 M-MLV 逆轉錄酶(M-MLV reverse transcriptase)) RScript cDNA 合成試劑盒(200 U/μl) |
50 rxns |
| RT001-0050 RT逆轉錄酶 |
RScript™ Reverse Transcriptase 10,000U (新一代工程重組 M-MLV 逆轉錄酶(M-MLV reverse transcriptase)) 逆轉錄酶 RScript Reverse Transcriptase |
50 rxns 10,000U/50μl |
| RT001-0250 RT逆轉錄酶 |
RScript™ Reverse Transcriptase 50,000U (新一代工程重組 M-MLV 逆轉錄酶(M-MLV reverse transcriptase)) 逆轉錄酶RScript Reverse Transcriptase |
250 rxns 50,000U/250μl |
| RI001-0125 RNase Inhibitor |
RIBOAssure™ RNase Inhibitor 5,000U (抑制真核酶如 RNase A、RNase B 和 RNase C 所表現出的核糖核酸酶 (RNase) 活性) RNase 抑制劑 |
125 rxns 5,000U/125μl |
| QPR01-0100 (Probe) |
PanProbes™ One-Step RT-qPCR Kit (Probe) (新一代工程重組 M-MLV 逆轉錄酶(M-MLV reverse transcriptase)) 強大的 RNase 抑制劑混合物(RNase inhibitors mix) 增強的抗體介導的熱啟動 DNA 聚合酶 (針對基於探針的real-time PCR 進行了優化) 一步驟 RT-qPCR 試劑盒 |
100 reactions (20 μl vol) |
| QPD01-0100 (Probe) |
PanProbes™ Universal qPCR MasterMix (針對基於probe- PCR (probe-based real-time PCR )進行了優化) 增強的抗體介導的熱啟動 DNA 聚合酶 PanProbes™ Universal qPCR預混液 |
100 reactions (20 μl vol) |
| QSR01-0100 (SYBR® Green) |
PanGreen™ One-Step RT-qPCR Kit (SYBR® Green) (新一代工程重組 M-MLV 逆轉錄酶(M-MLV reverse transcriptase)) 強大的 RNase 抑制劑混合物(RNase inhibitors mix) 增強的抗體介導的熱啟動 DNA 聚合酶 Universal SYBR® Green Master Mix 2 倍濃縮的即用型混合液 Master Mix 一個步驟RT-qPCR 試劑盒PanGreen |
100 reactions (20 μl vol) |
| QSD01-0100 SYBR® Green) |
PanGreen™ Universal SYBR® Green Mastermix (SYBR® Green) (2 倍濃縮的即用型 Master Mix 混合液) 增強的抗體介導的熱啟動 DNA 聚合酶 即用型 qPCR 混合液PanGreen |
100 reactions (20 μl vol) |
| PDR03-0100 | Total RNA Isolation Reagent (Reagent Based) for the tissue, cultured animal and bacterial cells, blood, and serum. Total RNA 分離試劑 (Reagent Based） |
100 rxns |
PCR混合液 | 100bp-10kb DNA Ladder | 瓊脂膠體粉Agarose | DNA核酸染劑 | 藍光觀察箱(含白光) | Mupid-2 水平電泳槽 |
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| CAT# | NAME | Size |
| BP001CU | BluPAD, LED Transilluminator, with mini Darkroom (CE/ETL, Patent No: M543442) |
Set |
| PMI09-0500 | IRIS9 Plus Prestained Protein Ladder (9 pre-stained bands, 15-170 kDa) |
500 μl |
| PMI11-0500 | IRIS11 Prestained Protein Ladder (11 pre-stained bands, 3-260 kDa) |
500 μl |
| PMB01-0500 | Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa) |
500 μl |
| PMB10-0500 | Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa) |
500 μl |
| PMB11-0500 | Blu11 Prestained Protein Ladder (11 pre-stained bands, 10-180 kDa) |
500 μl |
| PMB12-0500 | Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa) |
500 μl |
| PMB13-0500 | Blu13 Prestained Protein Ladder (13 pre-stained bands, 5-245 kDa) |
500 μl |
| PMU12-0500 | UNveil Unstained Protein Ladder, Broad Range (12 unstained bands, 10-200 kDa) |
500 μl |
| CCH321-B100ML | UltraScence Pico Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH345-B100ML | UltraScence Pico Ultra Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH365-B100ML | UltraScence Femto Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH375-B100ML | UltraScence Femto Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| PS002-B500ML | COOMASSIEnano Protein Staining Solution, 500ml | 500 ml |
| PS003-B500ML | Ponceau S Protein Staining Solution | 500 ml |
| BS001-B500ML | OneStep Blocker Western Blocking Solution and Signal Enhancer |
500 ml |
| TM001-B200ML | TMB ELISA Substrate | 100 ml (50ml+50ml) |
| DM001-R500 | BH 100bp DNA Ladder RTU (11 bands, 100-1,500bps) |
500 μl |
| DM003-R500 | BH 100bp DNA Ladder H3 RTU (12 bands, 100-3,000bps) |
500 μl |
| DM012-R500 | BH 50bp DNA Ladder RTU (17 bands, 50-1,500bps) |
500 μl |
| DM015-R500 | BH 1Kb Plus DNA Ladder RTU (13 bands, 100-10,000bps) |
500 μl |
| DMF12-0100 | OmniMARK 100 DNA Ladder RTU (12 bands, 100-3,000bps, fluorescent dye) |
600 μl |
| DMF13-0100 | OmniMARK 1K DNA Ladder RTU (13 bands, 100-10,000bps, fluorescent dye) |
600 μl |
| LD011-1000 | Prime Juice Preloading Fluorescent Stain | 1 ml |
| LD003-0500 | Novel Green Plus (20,000X) DNA Staining Reagent | 500 μl |
| AGT002-0500 | Agarose Tablets, 110 pcs | 0.5g x 110 |
| MB755-0100 | Agarose Powder 100g -Molecular Biology Grade | 100 g |
| MB755-0500 | Agarose Powder 500g -Molecular Biology Grade | 500 g |
| MB101-0500 | Taq DNA Polymerase | 500U/100μl |
| DP001-0100 | nanoTaq™ Hot-Start DNA Polymerase | 100 rxns 500U/100μl |
| DN001-0250 | 100 mM dNTP Set | 4 x 250μl |
| DN0010 | 10 mM dNTP Mix | 1 ml |
| MB200-P100 | 2X PCR SuperMix | 100 rxns 2 x 1.25mL |
| MBA01-0100 | OmniPCR Supermix w Fluorescent dye | 100 rxns (2.5ml) |
| RK001-0050 | RScript™ cDNA Synthesis Kit | 50 rxns |
| RT001-0050 | RScript™ Reverse Transcriptase 10,000U | 50 rxns 10,000U/50μl |
| RT001-0250 | RScript™ Reverse Transcriptase 50,000U | 250 rxns 50,000U/250μl |
| RI001-0125 | RIBOAssure™ RNase Inhibitor 5,000U | 125 rxns 5,000U/125μl |
| QPR01-0100 | PanProbes™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QPD01-0100 | PanProbes™ Universal qPCR MasterMix | 100 reactions (20 μl vol) |
| QSR01-0100 | PanGreen™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QSD01-0100 | PanGreen™ Universal SYBR® Green Mastermix | 100 reactions (20 μl vol) |
| PDP01-0100 | Plasmid miniPREP Kit | 100 rxns |
| PDP02-0020 | Plasmid midiPREP Kit | 20 rxns |
| PDP03-0010 | Plasmid maxiPREP Kit | 10 rxns |
| PDC01-0100 | PCR Clean-Up & Gel Extraction Kit | 100 rxns |
| PDC02-0100 | Genomic DNA isolation dual kit | 100 rxns |
| PDC05-0100 | Dual Genomic DNA Isolation Kit (Plant) | 100 rxns |
| PDC06-0100 | Dual Genomic DNA Isolation Kit(Tissue) | 100 rxns |
| PDC09-0100 | Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
| PDC10-0100 | Genomic DNA Isolation Kit (Plant) | 100 rxns |
| PDC11-0100 | Genomic DNA Isolation Kit (Tissue) | 100 rxns |
| PDC12-0100 | Genomic DNA Isolation Kit (Paraffin-embedded tissue) | 100 rxns |
| PDM01-0100 | Mbead Buffy Coat Genomic DNA Kit | 100 rxns |
| PDM02-0100 | Mbead Tissue Genomic DNA Kit | 100 rxns |
| PDM03-0100 | Mbead Bacteria Genomic DNA Kit | 100 rxns |
| PDM05-0100 | Mbead Plant Genomic DNA Kit | 100 rxns |
| PDM06-0100 | Mbead Blood/Cell Genomic DNA Kit | 100 rxns |
| PDR01-0100 | Genomic DNA Isolation Reagent | 100 rxns |
| PDR02-0100 | Plant Genomic DNA Isolation Reagent | 100 rxns |
| PDR05-0100 | Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue) | 100 rxns |
| PDC04-0100 | Total RNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
| PDC07-0100 | Total RNA Isolation Kit (Plant) | 100 rxns |
| PDC08-0100 | Total RNA Isolation Kit(Tissue) | 100 rxns |
| PDR03-0100 | Total RNA Isolation Reagent | 100 rxns |
| PDR04-0100 | Plant Total RNA Isolation Reagent | 100 rxns |
| PDC03-0100 | Virus Nucleic Acid Isolation Kit | 100 rxns |
| PDM04-0100 | Mbead Virus Genomic Nucleic Acid Kit | 100 rxns |
| QP019M-0050 | COVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa) | 50 rxns |
| QP019T-0100 | COVID-19 RT-qPCR Detection Kit Plus (TFDA) | 100 rxns |
| QP019-0100 | SARS-CoV-2/COVID-19 RT-qPCR Detection Kit (RUO) | 100 rxns |