Total RNA 分離試劑 (Reagent Based）|Bio-Helix 貨號PDR03-0100
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Total RNA 分離試劑 (Reagent Based）|Bio-Helix 貨號PDR03-0100
Total RNA Isolation Reagent (Reagent Based) for the tissue, cultured animal and bacterial cells, blood, and serum.
產品描述:
total RNA 分離試劑盒提供了一種高效的 3 步法，可從組織、培養的動物和細菌細胞、血液和血清中分離total RNA。這種獨特的試劑系統確保了從無限大小的樣品中獲得高產量和高質量的total RNA。如果需要更大量的樣品，試劑體積可以按比例縮放，使該試劑不僅非常易於使用，而且用途廣泛。無需RNA苯酚提取，整個過程可在60分鐘內完成。總 RNA 可用於 可用於 RT-PCR、Northern 印跡、cDNA 合成和Mapping。
Total RNA 分離試劑 (Reagent Based）產品特點
• Fast procedure delivering high-quality total RNA.
• Ready-to-use RNA for high performance in any downstream application.
• Consistent RNA yield from the starting material with a small amount.
• Provide sufficient reagents and 3 steps to treat the samples.
• Sample: Tissue, cultured animal and bacterial cells, blood, and serum.
Total RNA 分離試劑 (Reagent Based）產品應用
Molecular weight and size of DNA
Purity of DNA required
Downstream DNA applications
Time flexibility
Ease of DNA extraction technique or method
Expense reduction
Total RNA 分離試劑 (Reagent Based）操作方法 (以原廠操作手冊為優先)
Total RNA Isolation Protocol Sample Preparation
Tissue
1. Cut off 50 mg of the fresh tissue.
2. Grind the sample in the liquid nitrogen to a fine powder using a mortar and pestle.
Cultured Animal/Bacterial Cells
1. Transfer the cultured animal cells (up to 5 x 106 ) or bacterial culture (up to 1 x 109) to a 1.5 ml microcentrifuge tube.
2. Centrifuge at 14~16,000 x g for 1 minute and pour off the majority of the supernatant (If more than 1.5 ml of bacterial culture is used, repeat this step). Use the remaining supernatant to re-suspend the pellet.
Fresh Blood/Frozen Blood
1. Collect blood in the EDTA-NA2 treated collection tubes (or other anticoagulant mixtures).
2. Transfer up to 300 μl of the blood to a 1.5 ml microcentrifuge tube. If the blood sample is more than 300 μl (up to 1 ml), add the sample to a sterile 15 ml centrifuge tube.
Step 1 Lysis
Tissue
1. Add 500 μl of the GR Buffer 1 and 8 μl of the ß-mercaptoethanol to the sample in the mortar and grind the sample until it is completely dissolved.
2. Transfer the dissolved sample to a 1.5 ml microcentrifuge tube.
Cultured Animal and Bacterial Cells/Fresh Blood/Frozen Blood
Add 500 μl of the GR Buffer 1 and 8 μl of the ß-mercaptoethanol to the sample and mix completely
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| QSR01-0100 (SYBR® Green) |
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| QSD01-0100 SYBR® Green) |
PanGreen™ Universal SYBR® Green Mastermix (SYBR® Green) (2 倍濃縮的即用型 Master Mix 混合液) 增強的抗體介導的熱啟動 DNA 聚合酶 即用型 qPCR 混合液PanGreen |
100 reactions (20 μl vol) |
| PDR03-0100 | Total RNA Isolation Reagent (Reagent Based) for the tissue, cultured animal and bacterial cells, blood, and serum. Total RNA 分離試劑 (Reagent Based） |
100 rxns |
PCR混合液 | 100bp-10kb DNA Ladder | 瓊脂膠體粉Agarose | DNA核酸染劑 | 藍光觀察箱(含白光) | Mupid-2 水平電泳槽 |
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bio-helix.com
Bio-Helix成立於2007年，專注於為全球生命科學研究人員提供最好的生物試劑。包括 DNA 標記物、蛋白質標記物、預製蛋白質凝膠、核酸純化、PCR試劑等。與世界各地實驗室的科學家們一起努力，不斷提高質量，開始贏得客戶的信任和評價。品牌遍布四十多個國家，已成為生命科學實驗室值得信賴和歡迎的試劑品牌。Bio-Helix個願景，就是有一天我們的壽命可以延長；所有疾病的早期發現和隨後的適當治療都可以提高生活質量。中國古人認為，良醫治未病，治未病。在 Bio-Helix 中，創建了一個新品牌 LifeDirex 來實現這一願景。 LifeDirex 將專注於為診斷工具提供最好和最具創新性的試劑，以促進盡可能早地發現疾病。Bio-Helix使命是提供最優質的產品並開發新技術，幫助研究人員找到尋求科學成就的解決方案。此外，Bio-Helix認為現在是進入體外診斷設備（IVDD）市場的最佳時機。我們的目標是成為研究實驗室和IVDD的最佳解決方案提供商。
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