PanProbes™ Universal qPCR 預混液 MasterMix | Bio-Helix 貨號QPD01-0100
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PanProbes™ Universal qPCR預混液MasterMix| Bio-Helix 貨號QPD01-0100
PanProbes™ Universal qPCR MasterMix
產品描述:
PanProbesTM 通用 qPCR 預混液(Universal qPCR Master Mix)是一種 2 倍濃縮( 2x concentrated)的即用型預混液(ready-to-use master mix)，針對基於probe- PCR (probe-based real-time PCR )進行了優化，並與real-time PCR systems（不依賴 ROX 和依賴 ROX）兼容。它包含抗體介導的熱啟動 Taq DNA聚合酶( antibody-mediated hot-start Taq DNA polymerase)、dNTP、MgCl2、增強劑(enhancers)、穩定劑(stabilizers )和成功進行 PCR 反應的必需品。
* 增強的抗體介導的熱啟動 DNA 聚合酶。
* 針對基於探針的實時 PCR 進行了優化。
* 與大多數 QPCR 系統兼容。
Reaction Setup -Universal qPCR Master Mix
1. Thaw PanProbes™ Universal qPCR Master Mix and the rest of frozen reaction components to a temperature of 4°C. In order to entirely collect solutions, combine thoroughly and centrifuge briefly, then store at 4°C and avoid from light.
2. Prepare (on ice or at room temperature) enough assay Master Mix for all reactions by adding all necessary components, except the DNA template, according to the recommendations in Table 1 (below).
3. Combine the assay Master Mix thoroughly to ensure consistency and equally dispense the solution into each qPCR tube or into the wells of a qPCR plate. Employ good pipetting practice to ensure assay precision and accuracy.
4. Add DNA samples (and DNase-free H2O if needed) to the PCR tubes or wells containing assay Master Mix (Table 1), seal the tubes or wells with flat caps or optically transparent film. Note: to ensure thorough mixing of reaction components, vortex for approximately 30 seconds (or more).
5. Spin the tubes or plate to remove any air bubbles and collect the reaction mixture in the vessel bottom.
6. Setup the thermal cycling protocol on a real-time PCR instrument according to Table 2. Note: optimization may be needed for better performance.
7. Load the PCR tubes or plate into the real-time PCR instrument and commence the run.
8. Perform data analysis according to the instrument-specific instructions.
⚫ Process in the thermal cycler for 35~45 cycles as follows:
Figure 1. Performance of PanProbes™ Universal qPCR MasterMix
The SARS-CoV-2 nucleocapsid (N1) gene with serial diluted DNA template concentrations are prepared and conducted by qPCR. Based on the detected N1 probe fluorescence values, the result shows the Ct values corresponding to the different concentration.
| RK001-0050 cDNA合成KIT |
RScript™ cDNA Synthesis Kit (新一代工程重組 M-MLV 逆轉錄酶(M-MLV reverse transcriptase)) RScript cDNA 合成試劑盒(200 U/μl) |
50 rxns |
| RT001-0050 RT逆轉錄酶 |
RScript™ Reverse Transcriptase 10,000U (新一代工程重組 M-MLV 逆轉錄酶(M-MLV reverse transcriptase)) 逆轉錄酶 RScript Reverse Transcriptase |
50 rxns 10,000U/50μl |
| RT001-0250 RT逆轉錄酶 |
RScript™ Reverse Transcriptase 50,000U (新一代工程重組 M-MLV 逆轉錄酶(M-MLV reverse transcriptase)) 逆轉錄酶RScript Reverse Transcriptase |
250 rxns 50,000U/250μl |
| RI001-0125 RNase Inhibitor |
RIBOAssure™ RNase Inhibitor 5,000U (抑制真核酶如 RNase A、RNase B 和 RNase C 所表現出的核糖核酸酶 (RNase) 活性) RNase 抑制劑 |
125 rxns 5,000U/125μl |
| QPR01-0100 (Probe) |
PanProbes™ One-Step RT-qPCR Kit (Probe) (新一代工程重組 M-MLV 逆轉錄酶(M-MLV reverse transcriptase)) 強大的 RNase 抑制劑混合物(RNase inhibitors mix) 增強的抗體介導的熱啟動 DNA 聚合酶 (針對基於探針的real-time PCR 進行了優化) 一步驟 RT-qPCR 試劑盒 |
100 reactions (20 μl vol) |
| QPD01-0100 (Probe) |
PanProbes™ Universal qPCR MasterMix (針對基於probe- PCR (probe-based real-time PCR )進行了優化) 增強的抗體介導的熱啟動 DNA 聚合酶 PanProbes™ Universal qPCR預混液 |
100 reactions (20 μl vol) |
| QSR01-0100 (SYBR® Green) |
PanGreen™ One-Step RT-qPCR Kit (SYBR® Green) (新一代工程重組 M-MLV 逆轉錄酶(M-MLV reverse transcriptase)) 強大的 RNase 抑制劑混合物(RNase inhibitors mix) 增強的抗體介導的熱啟動 DNA 聚合酶 Universal SYBR® Green Master Mix 2 倍濃縮的即用型混合液 Master Mix 一個步驟RT-qPCR 試劑盒PanGreen |
100 reactions (20 μl vol) |
| QSD01-0100 SYBR® Green) |
PanGreen™ Universal SYBR® Green Mastermix (SYBR® Green) (2 倍濃縮的即用型 Master Mix 混合液) 增強的抗體介導的熱啟動 DNA 聚合酶 即用型 qPCR 混合液PanGreen |
100 reactions (20 μl vol) |
| PDR03-0100 | Total RNA Isolation Reagent (Reagent Based) for the tissue, cultured animal and bacterial cells, blood, and serum. Total RNA 分離試劑 (Reagent Based） |
100 rxns |
PCR混合液 | 100bp-10kb DNA Ladder | 瓊脂膠體粉Agarose | DNA核酸染劑 | 藍光觀察箱(含白光) | Mupid-2 水平電泳槽 |
bio-helix.com
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| CAT# | NAME | Size |
| BP001CU | BluPAD, LED Transilluminator, with mini Darkroom (CE/ETL, Patent No: M543442) |
Set |
| PMI09-0500 | IRIS9 Plus Prestained Protein Ladder (9 pre-stained bands, 15-170 kDa) |
500 μl |
| PMI11-0500 | IRIS11 Prestained Protein Ladder (11 pre-stained bands, 3-260 kDa) |
500 μl |
| PMB01-0500 | Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa) |
500 μl |
| PMB10-0500 | Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa) |
500 μl |
| PMB11-0500 | Blu11 Prestained Protein Ladder (11 pre-stained bands, 10-180 kDa) |
500 μl |
| PMB12-0500 | Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa) |
500 μl |
| PMB13-0500 | Blu13 Prestained Protein Ladder (13 pre-stained bands, 5-245 kDa) |
500 μl |
| PMU12-0500 | UNveil Unstained Protein Ladder, Broad Range (12 unstained bands, 10-200 kDa) |
500 μl |
| CCH321-B100ML | UltraScence Pico Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH345-B100ML | UltraScence Pico Ultra Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH365-B100ML | UltraScence Femto Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| CCH375-B100ML | UltraScence Femto Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
| PS002-B500ML | COOMASSIEnano Protein Staining Solution, 500ml | 500 ml |
| PS003-B500ML | Ponceau S Protein Staining Solution | 500 ml |
| BS001-B500ML | OneStep Blocker Western Blocking Solution and Signal Enhancer |
500 ml |
| TM001-B200ML | TMB ELISA Substrate | 100 ml (50ml+50ml) |
| DM001-R500 | BH 100bp DNA Ladder RTU (11 bands, 100-1,500bps) |
500 μl |
| DM003-R500 | BH 100bp DNA Ladder H3 RTU (12 bands, 100-3,000bps) |
500 μl |
| DM012-R500 | BH 50bp DNA Ladder RTU (17 bands, 50-1,500bps) |
500 μl |
| DM015-R500 | BH 1Kb Plus DNA Ladder RTU (13 bands, 100-10,000bps) |
500 μl |
| DMF12-0100 | OmniMARK 100 DNA Ladder RTU (12 bands, 100-3,000bps, fluorescent dye) |
600 μl |
| DMF13-0100 | OmniMARK 1K DNA Ladder RTU (13 bands, 100-10,000bps, fluorescent dye) |
600 μl |
| LD011-1000 | Prime Juice Preloading Fluorescent Stain | 1 ml |
| LD003-0500 | Novel Green Plus (20,000X) DNA Staining Reagent | 500 μl |
| AGT002-0500 | Agarose Tablets, 110 pcs | 0.5g x 110 |
| MB755-0100 | Agarose Powder 100g -Molecular Biology Grade | 100 g |
| MB755-0500 | Agarose Powder 500g -Molecular Biology Grade | 500 g |
| MB101-0500 | Taq DNA Polymerase | 500U/100μl |
| DP001-0100 | nanoTaq™ Hot-Start DNA Polymerase | 100 rxns 500U/100μl |
| DN001-0250 | 100 mM dNTP Set | 4 x 250μl |
| DN0010 | 10 mM dNTP Mix | 1 ml |
| MB200-P100 | 2X PCR SuperMix | 100 rxns 2 x 1.25mL |
| MBA01-0100 | OmniPCR Supermix w Fluorescent dye | 100 rxns (2.5ml) |
| RK001-0050 | RScript™ cDNA Synthesis Kit | 50 rxns |
| RT001-0050 | RScript™ Reverse Transcriptase 10,000U | 50 rxns 10,000U/50μl |
| RT001-0250 | RScript™ Reverse Transcriptase 50,000U | 250 rxns 50,000U/250μl |
| RI001-0125 | RIBOAssure™ RNase Inhibitor 5,000U | 125 rxns 5,000U/125μl |
| QPR01-0100 | PanProbes™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QPD01-0100 | PanProbes™ Universal qPCR MasterMix | 100 reactions (20 μl vol) |
| QSR01-0100 | PanGreen™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
| QSD01-0100 | PanGreen™ Universal SYBR® Green Mastermix | 100 reactions (20 μl vol) |
| PDP01-0100 | Plasmid miniPREP Kit | 100 rxns |
| PDP02-0020 | Plasmid midiPREP Kit | 20 rxns |
| PDP03-0010 | Plasmid maxiPREP Kit | 10 rxns |
| PDC01-0100 | PCR Clean-Up & Gel Extraction Kit | 100 rxns |
| PDC02-0100 | Genomic DNA isolation dual kit | 100 rxns |
| PDC05-0100 | Dual Genomic DNA Isolation Kit (Plant) | 100 rxns |
| PDC06-0100 | Dual Genomic DNA Isolation Kit(Tissue) | 100 rxns |
| PDC09-0100 | Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
| PDC10-0100 | Genomic DNA Isolation Kit (Plant) | 100 rxns |
| PDC11-0100 | Genomic DNA Isolation Kit (Tissue) | 100 rxns |
| PDC12-0100 | Genomic DNA Isolation Kit (Paraffin-embedded tissue) | 100 rxns |
| PDM01-0100 | Mbead Buffy Coat Genomic DNA Kit | 100 rxns |
| PDM02-0100 | Mbead Tissue Genomic DNA Kit | 100 rxns |
| PDM03-0100 | Mbead Bacteria Genomic DNA Kit | 100 rxns |
| PDM05-0100 | Mbead Plant Genomic DNA Kit | 100 rxns |
| PDM06-0100 | Mbead Blood/Cell Genomic DNA Kit | 100 rxns |
| PDR01-0100 | Genomic DNA Isolation Reagent | 100 rxns |
| PDR02-0100 | Plant Genomic DNA Isolation Reagent | 100 rxns |
| PDR05-0100 | Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue) | 100 rxns |
| PDC04-0100 | Total RNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
| PDC07-0100 | Total RNA Isolation Kit (Plant) | 100 rxns |
| PDC08-0100 | Total RNA Isolation Kit(Tissue) | 100 rxns |
| PDR03-0100 | Total RNA Isolation Reagent | 100 rxns |
| PDR04-0100 | Plant Total RNA Isolation Reagent | 100 rxns |
| PDC03-0100 | Virus Nucleic Acid Isolation Kit | 100 rxns |
| PDM04-0100 | Mbead Virus Genomic Nucleic Acid Kit | 100 rxns |
| QP019M-0050 | COVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa) | 50 rxns |
| QP019T-0100 | COVID-19 RT-qPCR Detection Kit Plus (TFDA) | 100 rxns |
| QP019-0100 | SARS-CoV-2/COVID-19 RT-qPCR Detection Kit (RUO) | 100 rxns |