DNA核酸染劑 | Novel Green Plus (20000X) BIO-HELIX貨號LD003-0500
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DNA核酸染劑 | Novel Green Plus (20000X) BIO-HELIX貨號LD003-0500
Novel Green Plus 是下一代 DNA 結合染料,可確保高靈敏度和質量。與溴化乙錠 (Ethidium bromide, EtBr) 相比,Novel Green Plus 在紫外透照下是一種反應靈敏的 DNA 染色試劑,是檢測瓊脂糖凝膠(agarose gel)上 dsDNA 最靈敏的染料之一。染料是根據與 PCR 相關的特性設計的,例如染料的 PCR 抑制、穩定性和螢光光譜。Novel Green Plus 為用戶帶來更可靠和更安全的體驗,因為染色的凝膠可以用藍光透照儀可視化,從而避免了皮膚/眼睛紫外線損傷的風險,並減少了 DNA 修飾引起的副作用。
Novel Green Plus is a next-generation DNA-binding dye which ensures high sensitivity and quality. Compared with Ethidium bromide (EtBr), Novel Green Plus is a much responsive DNA staining reagent under the UV transillumination and is one of the most sensitive stains for detecting dsDNA on the agarose gel. The dye is designed based on the properties relevant to PCR, such as PCR inhibition, stability and fluorescence spectra of the dye. The Novel Green Plus brings a more reliable and safer experience for the user, since the stained gel can be visualized with the blue-light transilluminator, thus avoiding the risk of skin/eye damage as well as reducing the side effects of DNA modification caused by the UV light.
Note: Nucleic Acid Stain / Fluorescent Dye / Safe Dye / Post-Staining
PCR混合液 | 100bp-10kb DNA Ladder | 瓊脂膠體粉Agarose | DNA核酸染劑 | 藍光觀察箱(含白光) | Mupid-2 水平電泳槽 |
電泳後 DNA 染色
1. 在瓊脂糖凝膠上進行電泳。
Novel Green Plus 與 TAE(40mM Tris-乙酸鹽、1mM EDTA、pH 8)、TBE(89 mM Tris 鹼、89 mM 硼酸、1mM EDTA、pH 8)和 TE(20mM Tris 鹼、1mM EDTA、 pH 8) 緩衝液。
2. 以 1:20,000 的比例稀釋 Novel Green Plus 儲備試劑。儲備染料可以在 TE、TAE 或 TBE 緩衝液中稀釋。
如果染色液用水稀釋,應在 24 小時內使用。
緩衝溶液可以增加這種螢光染色染料的穩定性。
3. 用染色液覆蓋凝膠,室溫孵育 10-30 分鐘。
使用塑料容器。不要使用玻璃容器,因為它會吸附染色液中的大部分染料。用鋁箔覆蓋染色容器或將其置於黑暗中,以保護染色容器免受光照。在室溫下輕輕攪動凝膠。
染色時間會隨著凝膠的厚度和瓊脂糖的百分比而變化。無需脫色。
染色液可避光低溫保存一周或更長時間。
4. 用紫外線或藍光透照儀給凝膠拍照。
每次使用後/之前用去離子水和軟布清潔透照器的表面非常重要。否則,熒光染料可能會積聚在玻璃表面並導致高熒光背景。攝像機和 CCD 相機的光譜響應與黑白打印膠片不同,因此它可能不會表現出相同程度的靈敏度。
預電泳 DNA 染色 / 凝膠內 DNA 染色
1. 使用您的標準方案製備熔化的瓊脂糖凝膠溶液。
2. 用熔化的凝膠溶液稀釋 Novel Green Plus 20,000X,並在倒入凝膠容器之前充分混合。冷卻熔化的瓊脂糖凝膠,直到它可以用手處理。使用 Novel Green Plus Gel Stain 的澆注凝膠會呈現輕微的黃色外觀,這與染料強度有關。澆鑄的凝膠在 4℃ 避光條件下可穩定保存 3 天。三天后,敏感度會一天天下降。
3. 在瓊脂糖凝膠上進行電泳(避光)。
推薦電壓為 4–10 V/cm(陽極和陰極之間的距離)。電泳時避免使用高壓。高電壓會導致過熱並對染料產生不利影響。
在電泳過程中,染色染料流向陽極,因此由於染色染料較少,分子量較小的 DNA 條帶可能強度較弱。
4. 想像一下帶有紫外線或藍光透照儀的凝膠。
Novel Green Plus 在 497 nm 處被激發,但也在 248 nm 處顯示出二次激發峰(圖 1a)。與 DNA 結合後,Novel Green Plus 的螢光發射以 524 nm 為中心(圖 1b)。這些光譜特性使這種熒螢光染料能夠與各種凝膠讀數設備兼容,包括紫外落射和透射照明器、氬激光和汞弧燈激發凝膠掃描儀。
打開之前,必須將小瓶完全加熱至室溫,以確保二甲基亞砜 (DMSO) 完全解凍並且溶液均勻。為避免染色劑丟失,將解凍後的染色劑在微量離心機中短暫離心,使 DMSO 溶液沉積在小瓶底部。為方便起見,可將染色劑分成較小的等分試樣並冷凍。較小的等分試樣會更快解凍。
乾浴加熱水浴槽 | Finnpipette F3 微量分注器 | 試管振盪器 Vortex Mixer | Genereach迷你型微量離心機 | ELISA儀器
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CAT# | NAME | Size |
BP001CU | BluPAD, LED Transilluminator, with mini Darkroom (CE/ETL, Patent No: M543442) |
Set |
PMI09-0500 | IRIS9 Plus Prestained Protein Ladder (9 pre-stained bands, 15-170 kDa) |
500 μl |
PMI11-0500 | IRIS11 Prestained Protein Ladder (11 pre-stained bands, 3-260 kDa) |
500 μl |
PMB01-0500 | Blu10 Plus Prestained Protein Ladder (10 pre-stained bands, 6.5-270 kDa) |
500 μl |
PMB10-0500 | Blu10 Prestained Protein Ladder (10 pre-stained bands, 11-180 kDa) |
500 μl |
PMB11-0500 | Blu11 Prestained Protein Ladder (11 pre-stained bands, 10-180 kDa) |
500 μl |
PMB12-0500 | Blu12 Prestained Protein Ladder (12 pre-stained bands, 11-245 kDa) |
500 μl |
PMB13-0500 | Blu13 Prestained Protein Ladder (13 pre-stained bands, 5-245 kDa) |
500 μl |
PMU12-0500 | UNveil Unstained Protein Ladder, Broad Range (12 unstained bands, 10-200 kDa) |
500 μl |
CCH321-B100ML | UltraScence Pico Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
CCH345-B100ML | UltraScence Pico Ultra Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
CCH365-B100ML | UltraScence Femto Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
CCH375-B100ML | UltraScence Femto Plus Western Substrate (Patent No: US10,711,185) |
100 ml (50ml+50ml) |
PS002-B500ML | COOMASSIEnano Protein Staining Solution, 500ml | 500 ml |
PS003-B500ML | Ponceau S Protein Staining Solution | 500 ml |
BS001-B500ML | OneStep Blocker Western Blocking Solution and Signal Enhancer |
500 ml |
TM001-B200ML | TMB ELISA Substrate | 100 ml (50ml+50ml) |
DM001-R500 | BH 100bp DNA Ladder RTU (11 bands, 100-1,500bps) |
500 μl |
DM003-R500 | BH 100bp DNA Ladder H3 RTU (12 bands, 100-3,000bps) |
500 μl |
DM012-R500 | BH 50bp DNA Ladder RTU (17 bands, 50-1,500bps) |
500 μl |
DM015-R500 | BH 1Kb Plus DNA Ladder RTU (13 bands, 100-10,000bps) |
500 μl |
DMF12-0100 | OmniMARK 100 DNA Ladder RTU (12 bands, 100-3,000bps, fluorescent dye) |
600 μl |
DMF13-0100 | OmniMARK 1K DNA Ladder RTU (13 bands, 100-10,000bps, fluorescent dye) |
600 μl |
LD011-1000 | Prime Juice Preloading Fluorescent Stain | 1 ml |
LD003-0500 | Novel Green Plus (20,000X) DNA Staining Reagent | 500 μl |
AGT002-0500 | Agarose Tablets, 110 pcs | 0.5g x 110 |
MB755-0100 | Agarose Powder 100g -Molecular Biology Grade | 100 g |
MB755-0500 | Agarose Powder 500g -Molecular Biology Grade | 500 g |
MB101-0500 | Taq DNA Polymerase | 500U/100μl |
DP001-0100 | nanoTaq™ Hot-Start DNA Polymerase | 100 rxns 500U/100μl |
DN001-0250 | 100 mM dNTP Set | 4 x 250μl |
DN0010 | 10 mM dNTP Mix | 1 ml |
MB200-P100 | 2X PCR SuperMix | 100 rxns 2 x 1.25mL |
MBA01-0100 | OmniPCR Supermix w Fluorescent dye | 100 rxns (2.5ml) |
RK001-0050 | RScript™ cDNA Synthesis Kit | 50 rxns |
RT001-0050 | RScript™ Reverse Transcriptase 10,000U | 50 rxns 10,000U/50μl |
RT001-0250 | RScript™ Reverse Transcriptase 50,000U | 250 rxns 50,000U/250μl |
RI001-0125 | RIBOAssure™ RNase Inhibitor 5,000U | 125 rxns 5,000U/125μl |
QPR01-0100 | PanProbes™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
QPD01-0100 | PanProbes™ Universal qPCR MasterMix | 100 reactions (20 μl vol) |
QSR01-0100 | PanGreen™ One-Step RT-qPCR Kit | 100 reactions (20 μl vol) |
QSD01-0100 | PanGreen™ Universal SYBR® Green Mastermix | 100 reactions (20 μl vol) |
PDP01-0100 | Plasmid miniPREP Kit | 100 rxns |
PDP02-0020 | Plasmid midiPREP Kit | 20 rxns |
PDP03-0010 | Plasmid maxiPREP Kit | 10 rxns |
PDC01-0100 | PCR Clean-Up & Gel Extraction Kit | 100 rxns |
PDC02-0100 | Genomic DNA isolation dual kit | 100 rxns |
PDC05-0100 | Dual Genomic DNA Isolation Kit (Plant) | 100 rxns |
PDC06-0100 | Dual Genomic DNA Isolation Kit(Tissue) | 100 rxns |
PDC09-0100 | Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
PDC10-0100 | Genomic DNA Isolation Kit (Plant) | 100 rxns |
PDC11-0100 | Genomic DNA Isolation Kit (Tissue) | 100 rxns |
PDC12-0100 | Genomic DNA Isolation Kit (Paraffin-embedded tissue) | 100 rxns |
PDM01-0100 | Mbead Buffy Coat Genomic DNA Kit | 100 rxns |
PDM02-0100 | Mbead Tissue Genomic DNA Kit | 100 rxns |
PDM03-0100 | Mbead Bacteria Genomic DNA Kit | 100 rxns |
PDM05-0100 | Mbead Plant Genomic DNA Kit | 100 rxns |
PDM06-0100 | Mbead Blood/Cell Genomic DNA Kit | 100 rxns |
PDR01-0100 | Genomic DNA Isolation Reagent | 100 rxns |
PDR02-0100 | Plant Genomic DNA Isolation Reagent | 100 rxns |
PDR05-0100 | Genomic DNA Isolation Reagent Kit(Blood/Cultured Cell/Tissue) | 100 rxns |
PDC04-0100 | Total RNA Isolation Kit (Blood/Cultured Cell/Fungus) | 100 rxns |
PDC07-0100 | Total RNA Isolation Kit (Plant) | 100 rxns |
PDC08-0100 | Total RNA Isolation Kit(Tissue) | 100 rxns |
PDR03-0100 | Total RNA Isolation Reagent | 100 rxns |
PDR04-0100 | Plant Total RNA Isolation Reagent | 100 rxns |
PDC03-0100 | Virus Nucleic Acid Isolation Kit | 100 rxns |
PDM04-0100 | Mbead Virus Genomic Nucleic Acid Kit | 100 rxns |
QP019M-0050 | COVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa) | 50 rxns |
QP019T-0100 | COVID-19 RT-qPCR Detection Kit Plus (TFDA) | 100 rxns |
QP019-0100 | SARS-CoV-2/COVID-19 RT-qPCR Detection Kit (RUO) | 100 rxns |