Orcein 染色試劑盒（用於乙型肝炎和彈性纖維）試劑盒 Orcein Stain Kit 貨號HBK-1/HBK-2

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原廠連結：	https://www.scytek.com
相關下載：	Orcein-染色試劑盒操作手冊

特點

Orcein 染色試劑盒（用於乙型肝炎和彈性纖維）試劑盒 Orcein Stain Kit 貨號HBK-1/HBK-2

Orcein Stain 可用於組織學程序，以可視化乙型肝炎表面抗原 (HBsAg)、彈性纖維和銅相關蛋白。HBsAg 在細胞的細胞質區域表現為不規則形狀的聚集體。該試劑可用於福爾馬林固定、石蠟包埋的切片。

HBsAg：深紅色/棕色
彈性纖維：深紅色/棕色
銅 Assoc。蛋白質：深紅色/棕色
背景：淺紅色/紫色

HBK-1 Kit Contents:
Potassium Permanganate Sol. (5%), 30ml, Item #: PPG030
Sulfuric Acid Solution (3%), 30ml, Item #: SAQ030
Oxalic Acid Solution (2%), 125ml, Item #: OQB125
Orcein Solution, 125ml, Item #: OAA125
Differentiating Solution, 125ml, Item #: DSA125

HBK-2 Kit Contents:
Potassium Permanganate Sol. (5%), 30ml, Item #: PPG030
Sulfuric Acid Solution (3%), 30ml, Item #: SAQ030
Oxalic Acid Solution (2%), 30m, Item #: OQB030
Orcein Solution, 30ml x2, Item #: OAA030
Differentiating Solution, 30ml, Item #: DSA030

Orcein Stain Kit 操作手冊下載

Procedure：

Procedure: Prepare Oxidizing Immediately Prior to Beginning Procedure:

1. Deparaffinize sections if necessary and hydrate to distilled water.

2. Incubate slide in freshly prepared Oxidizing Solution for 10 minutes.

3. Rinse slide briefly in running tap water followed by 1 dip in distilled water.

4. Incubate slide in Oxalic Acid Solution (2%) for 10 minutes or until clear. Note: Section should be colorless following this step.

5. Rinse slide for 1 minute in running tap water followed by 2 dips in distilled water.

6. Incubate slide in coplin jar containing Orcein solution for 4-8 hours (2 hours is sufficient for elastin). Note: Ensure tissue is fully immersed in staining jar. Close lid to prevent evaporation.

7. Rinse slide in Alcohol, Reagent (70%).

8. Differentiate in Differentiating Solution for 10-60 seconds.

9. Dip slide in Alcohol, Reagent (70%) and check slide microscopically for proper differentiation. Note: Repeat step 8 if necessary.

10. Dehydrate quickly in 3 changes of absolute alcohol.

11. Clear, and mount in synthetic resin. Note: If darker staining is preferred: 1) Incubation time in Orcein solution may be increased. and/or 2) Differentiation may be omitted by replacing steps 7-9 with a simple rinse with deionized water.

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