SARS-CoV-2 S1蛋白-ACE2結合抑制劑篩選試劑盒 | 貨號K2050-100

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原廠連結：	https://www.biovision.com
相關下載：	SARS-CoV-2 S1蛋白-ACE2結合抑制劑篩選試劑盒操作手冊

特點

產品描述: 嚴重急性呼吸系統綜合症冠狀病毒2（Severe Acute Respiratory Syndrome Coronavirus 2, SARS-CoV-2）也稱為2019新型冠狀病毒（coronavirus , 2019-nCoV）或人冠狀病毒2019（HCoV-19或hCoV-19），是導致2019年冠狀病毒病（COVID）的原因-19）大流行。它是一種RNA病毒(RNA virus)，可引起人類嚴重的呼吸道疾病(severe respiratory diseases)。 SARS-CoV-2冠狀病毒包含四個主要結構蛋白(structural proteins)，即刺突（Spike , S），膜（Membrane , M），Envelope（E）和核殼蛋白（Envelope , N）。穗蛋白(Envelope)位於病毒體的外殼上並介導病毒進入，因此在誘導中和抗體(neutralizing antibodies)和保護性免疫(protective immunity)中起重要作用。 S蛋白由S1和S2亞基(subunits)組成。 S1亞基(S1 subunit)包含一個可以與宿主受體特異性結合的受體結合域，即血管緊張素轉化酶2（Angiotensin Converting Enzyme 2, ACE2），它可以促進病毒進入靶細胞，包括呼吸道(respiratory)或腸道上皮細胞(intestinal epithelial cells)，內皮細胞(endothelial cells)，肺泡單核細胞(alveolar monocytes)或巨噬細胞(macrophages)。受體識別(receptor recognition)步驟是病毒感染性(viral infectivity)，發病機理(pathogenesis)和宿主範圍(host range)的重要決定因素。因此，針對S1蛋白和ACE2相互作用的干預策略為包括小分子(small molecules)和治療性抗體(therapeutic antibodies)在內的疫苗接種(vaccination)或抗病毒策略提出了重要的目標。BioVision的SARS-CoV-2 S1蛋白-ACE2結合抑制劑篩選試劑盒(SARS-CoV-2 S1 Protein-ACE2 Binding Inhibitor Screening Kit)可用於篩選S1蛋白與人ACE2結合的潛在抑制劑。在該測定中，使用鏈黴親和素-HRP檢測S1蛋白與生物素化的人ACE2的結合。隨後，添加TMB底物以可視化HRP酶促反應，從而生成藍色產物，一旦添加終止溶液，該產物將變為黃色。黃色的密度與S1蛋白與人ACE2的結合成正比。然而，在有效抑制劑的存在下，S1蛋白與人ACE2的結合被抑制，從而防止了顏色的產生。該測定試劑盒適用於96孔形式，可為高通量篩選S1蛋白與人ACE2結合的潛在抑製劑提供可靠的測試方法。此產品需由儀器進行定量 ELISA儀器設備請按此 (405、450、492、630 nm) 。

Cat # +Size	K2050-100
Size	100 assays
Kit Summary	BioVision’s SARS-CoV-2 S1 Protein-ACE2 Binding Inhibitor Screening Kit can be used to screen for potential inhibitors of S1 protein binding to human ACE2. In this assay, the binding of S1 protein to biotinylated human ACE2 is detected using Streptavidin-HRP. Subsequently, a TMB substrate is added to visualize the HRP enzymatic reaction thereby generating a blue colored product that changes to yellow once the stop solution is added. The density of the yellow color is proportional to the binding of S1 protein to Human ACE2. However, in the presence of potent inhibitor(s), the binding of S1 protein to Human ACE2 is suppressed thereby preventing the color generation. The assay kit is adapted to a 96-well format and provides a reliable test for high throughput screening of potential inhibitors of S1 protein binding to Human ACE2.
Detection Method	Absorbance at 450 nm
Species Reactivity	Human
Applications	• Screening or characterizing inhibitors of SARS-CoV-2, S1 protein binding to human ACE2
Features & Benefits	• Simple and easy to use • Provides a reliable test for high throughput screening of potential inhibitors of S1 protein binding to Human ACE2 • The assay kit is adapted to a 96-well format
Kit Components	• S1 Protein coated Microplate • Biotinylated Human ACE2 • Streptavidin-HRP • SARS-CoV-2 Inhibitor • TMB Substrate • Stop Solution • Wash Buffer (10X) • Assay Diluent • Plate Sealers
Storage Conditions	-20ºC
Shipping Conditions	Gel Pack

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